Downregulation of involucrin in psoriatic lesions following therapy with propylthiouracil, an anti‐thyroid thioureylene: immunohistochemistry and gene expression analysis

Gnanaraj, Pushpa; Dayalan, Haripriya; Elango, Tamilselvi; Malligarjunan, Hemamalini; Raghavan, Vijayashree; Rao, Ramesh

doi:10.1111/ijd.12565

Cited by 16 publications

(15 citation statements)

References 21 publications

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“…Since SIRT1 mediated effects are tissue specific, we attempted to increase SIRT1 levels and improve differentiation in psoriatic keratinocytes, by treating the cells with fructose. In the recent past our team has proved that PTU cleared lesions in psoriatic patients and improved differentiation as confirmed by modulated levels of involucrin [10] , [11] . In this study, we have focused on the role of fructose and PTU to improve differentiation in psoriatic keratinocytes and further explored the impact of these compounds on SIRT1 levels, a promoter of keratinocyte differentiation.…”

Section: Introductionmentioning

confidence: 87%

Induction of differentiation in psoriatic keratinocytes by propylthiouracil and fructose

et al. 2016

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Psoriasis is characterized by uncontrolled proliferation and poor differentiation. Sirtuin1 (SIRT1) a class III deacetylase, crucial for differentiation in normal keratinocytes, is reduced in psoriasis. Down regulated SIRT1 levels may contribute to poor differentiation in psoriasis. In addition, the levels of early differentiation factors Keratin1 (K1) and Keratin10 (K10) are depleted in psoriasis. We attempted to study a possible effect of fructose, a SIRT1 upregulator and Propylthiouracil (PTU) to augment differentiation in psoriatic keratinocytes. Keratinocytes were cultured from lesional biopsies obtained from psoriatic patients and control cells were obtained from patients undergoing abdominoplasty. Cells were treated with fructose and PTU individually. K1 and K10 transcript levels were measured to evaluate early differentiation; SIRT1 protein expression was also studied to decipher its role in the mechanism of differentiation. The K1, K10 transcript levels, SIRT1 protein and transcript levels in fructose treated psoriatic keratinocytes were improved. This suggests keratinocyte differentiation was induced by fructose through SIRT1 upregulation. Whereas PTU induced differentiation, as confirmed by improved K1, K10 transcript levels followed a non-SIRT1 mechanism. We conclude that the use of fructose and PTU may be an adjunct to the existing therapies for psoriasis.

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Section: Introductionmentioning

confidence: 87%

Induction of differentiation in psoriatic keratinocytes by propylthiouracil and fructose

et al. 2016

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“…It may possibly affect the keratin synthesis process by binding to nuclear T3 receptors found in the skin. Also, it can downregulate the level of involucrine, a protein precursor, which has an increased expression in psoriasis (23). This is another aspect suggesting that thyroid hormones may have unknown effects in psoriasis etiopathogenesis.…”

Section: Discussionmentioning

confidence: 99%

Genetic and Hormonal Determinations in a Pair of Identical Twins with Early Onset Psoriasis Vulgaris: Case Report and a Brief Review of the Literature

Roman

Orăsan

Coneac

et al. 2017

Revista Romana De Medicina De Laborator

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“… 16 - 19 Although the mechanism of action of these anti-thyroid preparations is unclear, but clearly indicated that thyroid constituents have their definite role in the pathogenesis of psoriasis. 16 - 20 , 40 For these reasons, we have tested the specificity of experimentally induced antibodies against ROS-modified epitope(s) towards thyroid antigens and their oxidized forms. Not only these, we have also tested the specificity of induced antibodies towards native and ROS modified human DNA.…”

Section: Discussionmentioning

confidence: 99%

Recognition of oxidized albumin and thyroid antigens by psoriasis autoantibodies

Al‐Shobaili

Ahmed

Rasheed

2015

SMJ

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Objectives:To investigate the role of reactive-oxygen-species (ROS) induced epitopes on human-serum-albumin (HSA) and thyroid antigens in psoriasis autoimmunity.Methods:This study was performed in the College of Medicine, Qassim University, Buraidah, Saudi Arabia between May 2014 and February 2015. The study was designed to explore the role of ROS-induced epitopes in psoriasis autoimmunity. Singlet-oxygen (or ROS)-induced epitopes on protein (ROS-epitopes-albumin) was characterized by in-vitro and in-vivo. Thyroid antigens were prepared from rabbit thyroid, and thyroglobulin was isolated from thyroid extract. Immunocross-reactions of protein-A purified anti-ROS-epitopes-HSA-immunoglobulin G (IgGs) with thyroid antigen, thyroglobulin, and their oxidized forms were determined. Binding characteristics of autoantibodies in chronic plaque psoriasis patients (n=26) against ROS-epitopes-HSA and also with native and oxidized thyroid antigens were screened, and the results were compared with age-matched controls (n=22).Results:The anti-ROS-epitopes-HSA-IgGs showed cross-reactions with thyroid antigen, thyroglobulin and with their oxidized forms. High degree of specific binding by psoriasis IgGs to ROS-epitopes-HSA, ROS-thyroid antigen and ROS-thyroglobulin was observed. Immunoglobulin G from normal-human-controls showed negligible binding with all tested antigens. Moreover, sera from psoriasis patients had higher levels of carbonyl contents compared with control sera.Conclusion:Structural alterations in albumin, thyroid antigens by ROS, generate unique neo-epitopes that might be one of the factors for the induction of autoantibodies in psoriasis.

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Downregulation of involucrin in psoriatic lesions following therapy with propylthiouracil, an anti‐thyroid thioureylene: immunohistochemistry and gene expression analysis

Cited by 16 publications

References 21 publications

Induction of differentiation in psoriatic keratinocytes by propylthiouracil and fructose

Induction of differentiation in psoriatic keratinocytes by propylthiouracil and fructose

Genetic and Hormonal Determinations in a Pair of Identical Twins with Early Onset Psoriasis Vulgaris: Case Report and a Brief Review of the Literature

Recognition of oxidized albumin and thyroid antigens by psoriasis autoantibodies

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