Downregulation of microRNA‑3934‑5p induces apoptosis and inhibits the proliferation of neuroblastoma cells by targeting TP53INP1

Ye, Wei; Liang, Fulv; Chen, Ying; Zhang, Maolin; Feng, Dongbo; Jiang, Xinyu

doi:10.3892/etm.2019.8007

Cited by 12 publications

(14 citation statements)

References 44 publications

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“…Therefore, we focused on TP53INP1 for further exploration. This study showed that the TP53INP1 expression in OSCC is consistent with former research that it is often lost during cancer development from different organs [42][43][44] . It has been documented that TP53INP1 exerts tumor suppressor function through involvement in cell death, cell-cycle arrest and cellular migration [43][44][45].…”

Section: Discussionsupporting

confidence: 92%

WITHDRAWN: MicroRNA-155-5p Contributes to 5-Fluorouracil Resistance Through Down-Regulating TP53INP1 in Oral Squamous Cell Carcinoma

Liu¹,

Hu²,

Zhao³

et al. 2021

Preprint

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The anticancer drug 5-fluorouracil (5-FU) resistance is a major obstacle to reducing the effectiveness of cancer treatment, and its detailed mechanism has not been fully elucidated. Here, in 5-FU-resistant human oral squamous cell carcinoma (OSCC) HSC3 cells (HSC3/5-FU), the levels of 21 miRNA candidates were detected and miR-155-5p level increased strikingly in HSC3/5-FU cells compared to HSC3 cells. Compared with HSC3 cells, the HSC3/5-FU cells transfected with miR-155-5p inhibitors decreased 5-FU IC50. Ectopic expression of miR-155-5p in HSC3 and HSC4 cells increased 5-FU IC50, migration and invasion abilities. Seven miR-155-5p target candidates were discovered by miRNA prediction algorithms, and in HSC3/5-FU cells TP53INP1 showed the lowest mRNA expression level compared with HSC3 cells. Ectopic expression of miR-155-5p in HSC3 and HSC4 cells decreased TP53INP1 expression level, and luciferase reporter assay further determined the interference effect of miR-155-5p on TP53INP1 expression. The enhancement of cell viability, migration and invasion by miR-155-5p after 5-FU treatment was reversed by TP53INP1 overexpression. After treatment with 5-FU, HSC3-miR-155-5p tumor-bearing nude mice presented growing tumors, while HSC3-TP53INP1 group possessed shrinking tumors. In conclusion, these results lead to the proposal that miR-155-5p enhances 5-FU resistance by decreasing TP53INP1 expression in OSCC.

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Section: Discussionsupporting

confidence: 92%

WITHDRAWN: MicroRNA-155-5p Contributes to 5-Fluorouracil Resistance Through Down-Regulating TP53INP1 in Oral Squamous Cell Carcinoma

Liu¹,

Hu²,

Zhao³

et al. 2021

Preprint

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“…In addition, it has been reported that miRNAs regulate cell viability by regulating TP53INP1 in other diseases. For example, Ye et al ( 38 ) reported that miR-3934-5p may inhibit the cell viability of neuroblastoma by targeting TP53INP1. TP53INP1 expression was negatively regulated by miR-301a, and TP53INP1/miR-301a was involved in gastric cancer cell viability ( 39 ).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‑1323 serves as a biomarker in gestational diabetes mellitus and aggravates high glucose‑induced inhibition of trophoblast cell viability by suppressing TP53INP1

2021

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Gestational diabetes mellitus (GDM) leads to poor pregnancy outcomes, and microRNAs (miRNAs/miRs) have been suggested to be associated with GDM, but the pathological mechanisms remain unclear. The present study aimed to investigate the diagnostic value of miR-1323 in GDM patients and its effects on trophoblast cell viability. Additionally, the present study investigated the correlation between miR-1323 and TP53INP1 to understand the pathological mechanism of GDM progression. Reverse transcription-quantitative polymerase chain reaction was used to detect the miR-1323 expression and TP53INP1 mRNA expression. The diagnostic value of serum miR-1323 was evaluated by receiver operating characteristic analysis. HTR-8/SVneo and BeWo cells were treated with high glucose (HG) to construct cell models of GDM, and trophoblast cell viability was assessed using an MTT assay. The protein expression of TP53INP1 was detected by western blot analysis. The correlation between miR-1323 and TP53INP1 was investigated by luciferase reporter assay. The miR-1323 expression was increased in patients with GDM, which had relatively high diagnostic accuracy for GDM screening and was positively correlated with fasting blood glucose in patients GDM. HG upregulated the miR-1323 expression and inhibited trophoblast cell viability. Overexpression of miR-1323 significantly inhibited the viability of HG-induced trophoblast cells. TP53INP1, a target gene of miR-1323, was negatively correlated with miR-1323. TP53INP1 overexpression reversed the inhibitory effect of miR-1323 overexpression on the viability of HG-treated trophoblast cells. Increased levels of serum miR-1323 may be a diagnostic biomarker for GDM. Additionally, miR-1323 may inhibit trophoblast cell viability by inhibiting TP53INP1, suggesting that it may be a potential therapeutic target for GDM.

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“…At rst, M, H, et al [25]demonstrated that EGFR agonist EREG has played an important role in tamoxifen-resistant breast cancer cells by activating EGFR signaling and its downstream glycolytic genes. It was further proved that EREG was a direct target of miR-186-3p, and tamoxifen downregulation of miR-186-3p could lead to upregulation of EREG in tamoxifen-resistant breast cancer cells [26]. Research by W, Y et al [26] found that miR-3934-5p…”

Section: Discussionmentioning

confidence: 99%

“…It was further proved that EREG was a direct target of miR-186-3p, and tamoxifen downregulation of miR-186-3p could lead to upregulation of EREG in tamoxifen-resistant breast cancer cells [26]. Research by W, Y et al [26] found that miR-3934-5p…”

Section: Discussionmentioning

confidence: 99%

Development and Validation of A Seven-microRNA Signature as a Novel Prognostic Biomarker for Bladder Cancer

Lin

et al. 2020

Preprint

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Background The differential expression of miRNAs has played a significant role in bladder tumors. The aim of our study was to screen new biomarkers . Methods Through differential analysis of bladder cancer mRNA and miRNA expression data in the TCGA, differential genes and miRNAs were screened. Furthermore, Cox univariate analysis and multifactor analysis were used to establish a prognostic prediction model . The predictive ability of the prognostic model was then verified on the patient. The action mechanism of these miRNAs was analyzed.Results By the differential analysis and standardization of miRNA expression profiles. Differentially expressed miRNAs were screened, then all the patients were then randomly divided into train group and the test group. 23 miRNAs were revealed , then a Seven-miRNA signature prognostic biomarkers was constituting.Univariate cox regression and multivariate cox regression considering other clinical factors displayed that the seven-miRNA signature could serve as an independent prognostic factor.Target genes of these seven miRNAs were analyzed by KEGG signaling pathway and GO enrichment analysis. . Conclusion The prognostic model constructed by seven miRNAs has possessed certain degree of sensitivity and specificity for the prediction of the survival of bladder cancer patients, which can be used as a potential new clinical marker for bladder cancer patients.

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Downregulation of microRNA‑3934‑5p induces apoptosis and inhibits the proliferation of neuroblastoma cells by targeting TP53INP1

Cited by 12 publications

References 44 publications

WITHDRAWN: MicroRNA-155-5p Contributes to 5-Fluorouracil Resistance Through Down-Regulating TP53INP1 in Oral Squamous Cell Carcinoma

WITHDRAWN: MicroRNA-155-5p Contributes to 5-Fluorouracil Resistance Through Down-Regulating TP53INP1 in Oral Squamous Cell Carcinoma

MicroRNA‑1323 serves as a biomarker in gestational diabetes mellitus and aggravates high glucose‑induced inhibition of trophoblast cell viability by suppressing TP53INP1

Development and Validation of A Seven-microRNA Signature as a Novel Prognostic Biomarker for Bladder Cancer

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