Downregulation of miR‑146a inhibits osteoporosis in the jaws of ovariectomized rats by regulating the Wnt/β‑catenin signaling pathway

Liu, Hua; Yue, Xianhu; Zhang, Gang

doi:10.3892/ijmm.2020.4839

Cited by 14 publications

(11 citation statements)

References 27 publications

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“…In this study, we used standard bilateral OVX rats, the most frequently used hormone-deficient osteoporosis animal model [ 26 ], to mimic osteoporosis observed in menopausal women, and then a hydrogel containing VPA implanted into the femoral condyle defect under the state of osteoporosis to simulate the state of bone defect in the human body. Interestingly, a similar phenomenon that the femoral condyle defect in OVX rats provide a poor bone regeneration with osteoporotic bone was also reported in our previous studies [ 27 , 28 ].…”

Section: Discussionmentioning

confidence: 99%

Hydrogel contained valproic acid accelerates bone-defect repair via activating Notch signaling pathway in ovariectomized rats

Tao

et al. 2021

J Mater Sci: Mater Med

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The purpose was to observe whether valproic acid (VPA) has a positive effect on bone-defect repair via activating the Notch signaling pathway in an OVX rat model. The MC3T3-E1 cells were cocultured with VPA and induced to osteogenesis, and the osteogenic activity was observed by alkaline phosphatase (ALP) staining, Alizarin Red (RES) staining and Western blotting (WB). Then the hydrogel-containing VPA was implanted into the femoral epiphysis bone-defect model of ovariectomized (OVX) rats for 12 weeks. Micro-CT, biomechanical testing, histology, immunofluorescence, RT-qPCR, and WB analysis were used to observe the therapeutic effect and explore the possible mechanism. ALP and ARS staining and WB results show that the cell mineralization, osteogenic activity, and protein expression of ALP, OPN, RUNX-2, OC, Notch 1, HES1, HEY1, and JAG1 of VPA group is significantly higher than the control group. Micro-CT, biomechanical testing, histology, immunofluorescence, and RT-qPCR evaluation show that group VPA presented the stronger effect on bone strength, bone regeneration, bone mineralization, higher expression of VEGFA, BMP-2, ALP, OPN, RUNX-2, OC, Notch 1, HES1, HEY1, and JAG1 of VPA when compared with OVX group. Our current study demonstrated that local treatment with VPA could stimulate repair of femoral condyle defects, and these effects may be achieved by activating Notch signaling pathway and acceleration of blood vessel and bone formation.

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Section: Discussionmentioning

confidence: 99%

Hydrogel contained valproic acid accelerates bone-defect repair via activating Notch signaling pathway in ovariectomized rats

Tao

et al. 2021

J Mater Sci: Mater Med

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“…Sp are the main indicators to evaluate the spatial morphological structure of trabeculae, and SMI is a parameter describing the proportion of plate layer structure and rod structure of the trabecular [17] . The performance of bone pathological including the N. Ob, BA/TA decreased, while the N.Oc, Oc.S/BS increased [18] . Our results showed that significantly higher BMD, BV/TV, Tb.…”

Section: Discussionmentioning

confidence: 93%

IGF-1 c.258 A > G synonymous mutation ameliorates senile osteoporosis

Hao,

Wang,

Dai

et al. 2024

Preprint

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Senile osteoporosis SOP is a multifactorial, age-related progressive phenomenon that leads to an increased susceptibility to fragility fractures. IGF-1 is an important regulator of bone reconstruction and metabolism. Nevertheless, we unexpectedly found IGF-1 c.258A>G synonymous mutation did not alter bone growth at sexual maturation under expression change of IGF-1. Considering the RNA-seq results that c.258A>G involved in cellular senescence, we suspected c.258A>G may participate in SOP. Therefore, the effect of IGF-1 c.258A>G on SOP was firstly detected by in vivo assays, the changes of BMD, bone formation and resorption index as well as the expression of related genes in Gal-induced SOP mice with two genotypes indicated it resisted SOP. Then, the in vitro study of H2O2-induced primary osteoblasts and MC3T3-E1 cells confirmed the IGF-1 c.258A>G ameliorates SOP by promoting the proliferation, migration, differentiation and mineralization. At last, co-culture system of two genotypes osteoblast and osteoclast further verified the mutation prevents SOP by increasing the bone formation capacity. Collectively, this study illuminated the role of IGF-1 c.258A>G in ameliorating SOP, which may be identified as an adjuvant therapy target for SOP. To our knowledge, this is one of the few studies that have been done in animals on the function of synonymous mutations.

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“…In addition, glabrene therapy completely reversed the OVX‐induced drop in the expression of the proteins Lrp5, β‐catenin, Runx2, and Osx. The results of the current study suggested that glabrene could be an alternative treatment option for the prevention and treatment of postmenopausal osteoporosis by triggering the Wnt/β‐catenin signaling pathway (Liu et al, 2021).…”

Section: Other Diseasesmentioning

confidence: 84%

A review on phytoestrogens: Current status and future direction

Patra

Gorai

Pal

et al. 2023

Phytotherapy Research

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Phytoestrogens are plant secondary metabolite that is structurally and functionally similar to mammalian estrogens, which have been shown to have various health benefits in humans. Isoflavones, coumestans, and lignans are the three major bioactive classes of phytoestrogens. It has a complicated mechanism of action involving an interaction with the nuclear estrogen receptor isoforms ERα and ERβ, with estrogen agonist and estrogen antagonist effects. Depending on their concentration and bioavailability in various plant sources, phytoestrogens can act as estrogen agonist or antagonists. Menopausal vasomotor symptoms, breast cancer, cardiovascular disease, prostate cancer, menopausal symptoms, and osteoporosis/bone health have all been studied using phytoestrogens as an additional standard hormone supplemental remedy. The botanical sources, techniques of identification, classification, side effects, clinical implications, pharmacological and therapeutic effects of their proposed mode of action, safety issues, and future directions for phytoestrogens have all been highlighted in this review.

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Downregulation of miR‑146a inhibits osteoporosis in the jaws of ovariectomized rats by regulating the Wnt/β‑catenin signaling pathway

Cited by 14 publications

References 27 publications

Hydrogel contained valproic acid accelerates bone-defect repair via activating Notch signaling pathway in ovariectomized rats

Hydrogel contained valproic acid accelerates bone-defect repair via activating Notch signaling pathway in ovariectomized rats

IGF-1 c.258 A > G synonymous mutation ameliorates senile osteoporosis

A review on phytoestrogens: Current status and future direction

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Downregulation of miR‑146a inhibits osteoporosis in the jaws of ovariectomized rats by regulating the Wnt/β‑catenin signaling pathway

Cited by 14 publications

References 27 publications

Hydrogel contained valproic acid accelerates bone-defect repair via activating Notch signaling pathway in ovariectomized rats

Hydrogel contained valproic acid accelerates bone-defect repair via activating Notch signaling pathway in ovariectomized rats

IGF-1 c.258 A &gt; G synonymous mutation ameliorates senile osteoporosis

A review on phytoestrogens: Current status and future direction

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IGF-1 c.258 A > G synonymous mutation ameliorates senile osteoporosis