Downregulation of OIP5-AS1 affects proNGF-induced pancreatic cancer metastasis by inhibiting p75NTR levels

Li, Ang; Feng, Lei; Niu, Xiaoya; Zeng, Qihui; Li, Bei; You, Zhen

doi:10.18632/aging.202847

Cited by 9 publications

(7 citation statements)

References 51 publications

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“…Notably, lncRNA OIP5-AS1 exerts similar suppressing effects on targeting NGFR-regulated p75NTR 3 0 UTR mRNA, 21 these results remind us to investigate which areas of Oct4 mRNA were bound by OIP5-AS1. We then performed luciferase reporter combined with RNA-RNA interaction in vitro analysis, and revealed that lncRNA OIP5-AS1 directly bound to Oct4 mRNA 3 0 UTR.…”

Section: Discussionmentioning

confidence: 74%

“…Therefore, we concluded that lncRNA OIP5‐AS1 could act as an mRNA partner for Oct4 mRNA, a critical stemness regulator, and thus increase Oct4 mRNA stability and expression, this effect of OIP5‐AS1 on Oct4 mRNA is similar to that of miRNAs on transcripts. Notably, lncRNA OIP5‐AS1 exerts similar suppressing effects on targeting NGFR‐regulated p75NTR 3′UTR mRNA, 21 these results remind us to investigate which areas of Oct4 mRNA were bound by OIP5‐AS1. We then performed luciferase reporter combined with RNA–RNA interaction in vitro analysis, and revealed that lncRNA OIP5‐AS1 directly bound to Oct4 mRNA 3′UTR.…”

Section: Discussionmentioning

confidence: 74%

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Long noncoding RNA OIP5‐AS1 promotes the stemness of lung cancer cells through enhancing Oct4 mRNA stability

Mao

2022

Environmental Toxicology

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Long noncoding RNA (lncRNA) OIP5-AS1 was shown to facilitate drug resistance and metastasis in several tumors. As cancer stem cells (CSCs) have been elucidated as the origin of drug resistance and tumor progression, we speculate that lncRNA OIP5-AS1 holds critical roles in the CSC-like traits of lung cancer. Here, lncRNA OIP5-AS1 was found to be highly expressed in lung cancer cell spheres. Following experiments showed that OIP-AS1 knockdown reduced the CSC-like traits of lung cancer spheres, while overexpression of OIP-AS1 conferred the CSC-like traits in lung cancer cells by performing sphere-formation analysis, detecting stemness marker expression, and ALDH activity. Mechanistic studies revealed that lncRNA OIP5-AS1 could increase Oct4 expression by directly interacting with Oct4 mRNA and enhancing Oct4 mRNA stability. Finally, we found that the knockdown of Oct4 could rescue the promoting effects of OIP5-AS1 overexpression on the CSC-like traits of lung cancer. These results demonstrate that lncRNA OIP5-AS1 can confer lung cancer CSC-like traits by directly interacting with Oct4 mRNA and thus increasing Oct4 mRNA stability and expression.

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Section: Discussionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 74%

Long noncoding RNA OIP5‐AS1 promotes the stemness of lung cancer cells through enhancing Oct4 mRNA stability

Mao

2022

Environmental Toxicology

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“…Long noncoding RNAs have been accepted as promising biomarkers and targets in the prognosis and treatment of cancer patients [22,23]. Currently, most of the studies focused on screening lncRNA signature with certain traits, such as m6A methylation-regulated lncRNA and pyroptosis-related lncRNA.…”

Section: Discussionmentioning

confidence: 99%

Immune landscape of a prognostic immunogenic cell death (ICD)-related long noncoding RNAs (lncRNAs) signature in colon adenocarcinoma (COAD)

Liu

Fang

Lü

et al. 2022

Preprint

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Background Immunogenic cell death (ICD), as a form of regulated cell death, demonstrates promising roles in immunogenic tumor microenvironment. However, the expression and roles of ICD-related long noncoding RNAs (lncRNAs) in COAD were largely unknown. Methods RNA-sequencing data and clinical data were downloaded from The Cancer Genome Atlas (TCGA) database. A list of 34 immunogenic cell death-related genes was retrieved from published literatures. ICD-related lncRNAs were retrieved using R language and Perl software. Univariate and multivariate cox analyses were used to construct an ICD-related lncRNA risk model. The performance of thissignature was validated using Kaplan-Meier curve and receiver operating curve (ROC). The immune landscape of the risk signature was revealed by analyzing the correlations with immune cells infiltration, immune function, immune checkpoint inhibitors. Correlations with RNA stemness score (RNAss) were analyzed using Spearman correlation coefficient. Correlation with m6A regulators was investigated using Wilcoxon test. Moreover, expression and survival analysis of the risk lncRNAs were revealed based on TCGA database. Results We identified 23 ICD-related lncRNAs with prognostic values to construct a risk model. The risk model demonstrated good performance in predicting survival. The area under curve (AUC) of the ROC curve at 1-, 2- and 3-year were 0.684, 0.715, and 0.747, respectively. Further univariate and multivariate analysis showed that the risk score was an independent prognosis factor. Further validation showed that there were significant differences in immune cells infiltration, immune functions, and immune checkpoint inhibitor expression between high- and low-risk groups. In addition, there was negative correlation between the risk signature and RNAss, and there was difference in the expression of m6A regulators in high- and low-risk groups. A nomogram including the risk score also demonstrating significant prognostic value. Moreover, upregulated expression of MIR4435-2HG, LINC02257, AL161729.4, AC007128.1 and AP001619.1 predicted poor overall survival in COAD. Conclusions We established an ICD-related lncRNA signature for the overall survival of COAD, which demonstrating high sensitivity and specificity. The correlations between ICD-related lncRNAs and tumor immune microenvironment still need to be further investigated.

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“…ENSG00000234456 (MAGI2-AS3) has been found to be involved in multiple cancers, including esophageal cancer (34), bladder cancer (35), breast cancer (36), and colon adenocarcinoma (37). Compared to other CC-related lncRNA biomarkers (38,39), these four lncRNAs are relatively novel and have good prognostic efficacy. Therefore, we hope that our results will be helpful in identifying potential targeting prognostic lncRNAs for m6A regulators, thus providing insights for their deeper study in CC tumorigenesis and progression.…”

Section: Discussionmentioning

confidence: 99%

N6-Methylandenosine-Related lncRNAs in Tumor Microenvironment Are Potential Prognostic Biomarkers in Colon Cancer

Zhang

Zhao²,

Li³

et al. 2021

Front. Oncol.

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BackgroundLncRNA dysregulation and the tumor microenvironment (TME) have been shown to play a vital role in the progression and prognosis of colon cancer (CC). We aim to reveal the potential molecular mechanism from the perspective of lncRNA in the TME and provide the candidate biomarkers for CC prognosis.MethodsESTIMATE analysis was used to divide the CC patients into high and low immune or stromal score groups. The expression array of lncRNA was re-annotated by Seqmap. Microenvironment-associated lncRNAs were filtered through differential analysis. The m6A-associated lncRNAs were screened by Pearson correlation analysis. Lasso Cox regression analyses were performed to construct the m6A- and tumor microenvironment-related lncRNA prognostic model (m6A-TME-LM). Survival analysis was used to assess the prognostic efficacy of candidate lncRNAs. Enrichment analyses annotated the candidate genes’ functions.ResultsWe obtained 25 common differentially expressed lncRNAs (DELs) associated with immune microenvironment and m6A-related genes for subsequent lasso analysis. Four out of these DELs were selected for the m6A-TME-LM. All the four lncRNAs were related to overall survival, and a test set testified the result. Further stratification analysis of the m6A-TME-LM retained its ability to predict OS for male and chemotherapy adjuvant patients and performed an excellent prognostic efficacy in the TNM stage III and IV subgroups. Network analysis also found the four lncRNAs mediated co-expression network was associated with tumor development.ConclusionWe constructed the m6A-TME-LM, which could provide a better prognostic prediction of CC.

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Downregulation of OIP5-AS1 affects proNGF-induced pancreatic cancer metastasis by inhibiting p75NTR levels

Cited by 9 publications

References 51 publications

Long noncoding RNA OIP5‐AS1 promotes the stemness of lung cancer cells through enhancing Oct4 mRNA stability

Long noncoding RNA OIP5‐AS1 promotes the stemness of lung cancer cells through enhancing Oct4 mRNA stability

Immune landscape of a prognostic immunogenic cell death (ICD)-related long noncoding RNAs (lncRNAs) signature in colon adenocarcinoma (COAD)

N6-Methylandenosine-Related lncRNAs in Tumor Microenvironment Are Potential Prognostic Biomarkers in Colon Cancer

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