Downregulation of Piwil3 suppresses cell proliferation, migration and invasion in gastric cancer

Jiang, Lei; Wang, Wenjun; Li, Zhanwu; Wang, Xiaozhou

doi:10.3233/cbm-170324

Cited by 23 publications

(23 citation statements)

References 16 publications

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“…Then, the IHC staining score was obtained by using the H-score system. The cytoplasmic expression of RNF180 was assessed by assigning scores to the average intensity of positive tumor cells 15 .…”

Section: Immunohistochemistrymentioning

confidence: 99%

RNF180 mediates STAT3 activity by regulating the expression of RhoC via the proteasomal pathway in gastric cancer cells

Liu

Sun

et al. 2020

Cell Death Dis

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Ring finger protein 180 (RNF180) is an important member of the E3 ubiquitin ligase family. As a tumor suppressor gene, RNF180 is significantly associated with the prognosis of patients with gastric cancer (GC) and can inhibit the proliferation, invasion, and migration of GC cells. Signal transducer and activator of transcription 3 (STAT3) are considered one of the most common oncogenes in human cancers with a key role in GC progression. In this study, we explored the molecular signaling pathways by which RNF180 could potentially regulate STAT3 through transcriptomics and proteomics experiments. Here, we found RNF180 overexpression could suppress STAT3 phosphorylation in GC cells. Ubiquitin label-free experiments showed that the ubiquitination level of Ras homolog gene family member C (RhoC) is significantly increased in GC cells transfected with an RNF180 expression vector (RNF180-GFP vector) compared with cells transfected with an empty vector (vehicle vector). We subsequently demonstrated that RNF180 could directly combine with RhoC and promote the ubiquitination and degradation of RhoC protein in GC cells. The phosphorylation level of STAT3 significantly decreased in GC cells after RhoC knockdown using small hairpin RNA (shRNA). Together, these results reveal RNF180 could inhibit GC progression by reducing the phosphorylation of STAT3 via the ubiquitination and degradation of RhoC protein in GC cells. Thus, the protein may be considered a novel therapeutic target for patients with GC.

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Section: Immunohistochemistrymentioning

confidence: 99%

RNF180 mediates STAT3 activity by regulating the expression of RhoC via the proteasomal pathway in gastric cancer cells

Liu

Sun

et al. 2020

Cell Death Dis

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“…PIWIL3 are expressed at low levels in glioma tissues and negatively associate with glioma pathological grade [38]. PIWIL3 over-expression promotes GC cell proliferation, migration, and invasion whereas its down-regulation suppresses the progression of GC via the JAK2/STAT3 signaling pathway [114]. PIWIL3 protein is also increased in more aggressive primary malignant melanoma and metastatic disease, and thus may be involved in malignant melanoma progression [115].…”

Section: Introductionmentioning

confidence: 99%

The emerging role of the piRNA/piwi complex in cancer

et al. 2019

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Piwi interacting RNAs (piRNAs) constitute novel small non-coding RNA molecules of approximately 24–31 nucleotides in length that often bind to members of the piwi protein family to play regulatory roles. Recently, emerging evidence suggests that in addition to the mammalian germline, piRNAs are also expressed in a tissue-specific manner in a variety of human tissues and modulate key signaling pathways at the transcriptional or post-transcriptional level. In addition, a growing number of studies have shown that piRNA and PIWI proteins, which are abnormally expressed in various cancers, may serve as novel biomarkers and therapeutic targets for tumor diagnostics and treatment. However, the functions of piRNAs in cancer and their underlying mechanisms remain incompletely understood. In this review, we discuss current findings regarding piRNA biogenetic processes, functions, and emerging roles in cancer, providing new insights regarding the potential applications of piRNAs and piwi proteins in cancer diagnosis and clinical treatment.

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“…The association of PIWIL2 via increasing the expression of CDK2 and cyclin A in cancer cells is reported in glioma and nonsmall lung cancer (NSCLC) cells [66]. PIWIL3 promotes the cancer proliferation, migration, and invasion through the JAK2/STAT3 signal pathway [67]. PIWIL4 can promote cancer cell division, migration, and survival of breast cancer by activating TGF-β, MAPK/ ERK, and FGF signaling pathways [68].…”

Section: Re Regulation In Normal Cells and Abnormal Reactivation And mentioning

confidence: 99%

Endogenous Retroelements in Cancer: Molecular Roles and Clinical Approach

Lee¹,

Cho²

2021

Methods in Molecular Medicine

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Retroelements have been considered as "Junk" DNA although the encyclopedia of DNA elements (ENCODE) project has demonstrated that most of the genome is functional. Since the contribution of LINE1 (L1) and human endogenous retrovirus (HERV) has been suspected to cause human cancers, their regulations and putative molecular functions have been investigated in diverse types of cancer. Their diagnostic, prognostic, and therapeutic potentials have been incessantly proposed using cancer associated or specific properties, such as hypomethylation, increased transcripts, and reverse transcriptase, as well as cancer-associated antigens. This chapter presents the current knowledge on retroelements in various aspects during tumorigenesis and their clinical usage in many cancer studies.

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Downregulation of Piwil3 suppresses cell proliferation, migration and invasion in gastric cancer

Abstract: These findings indicate that overexpression of Piwil3 promotes the proliferation, migration and invasion of GC cells partially through JAK2/STAT3 signal pathway.

Cited by 23 publications

References 16 publications

RNF180 mediates STAT3 activity by regulating the expression of RhoC via the proteasomal pathway in gastric cancer cells

RNF180 mediates STAT3 activity by regulating the expression of RhoC via the proteasomal pathway in gastric cancer cells

The emerging role of the piRNA/piwi complex in cancer

Endogenous Retroelements in Cancer: Molecular Roles and Clinical Approach

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