Downregulation of the microRNA-1/133a cluster enhances cancer cell migration and invasion in lung-squamous cell carcinoma via regulation of Coronin1C

Abstract

Lung cancer is clearly the primary cause of cancer-related deaths worldwide. Recent molecular-targeted strategy has contributed to improvement of the curative effect of adenocarcinoma of the lung. However, such current treatment has not been developed for squamous cell carcinoma (SCC) of the disease. The new genome-wide RNA analysis of lung-SCC may provide new avenues for research and the development of the disease. Our recent microRNA (miRNA) expression signatures of lung-SCC revealed that clustered miRNAs miR-1/133a were significantly reduced in cancer tissues. Here, we found that restoration of both mature miR-1 and miR-133a significantly inhibited cancer cell proliferation, migration and invasion. Coronin-1C (CORO1C) was a common target gene of the miR-1/133a cluster, as shown by the genome-wide gene expression analysis and the luciferase reporter assay. Silencing of CORO1C gene expression inhibited cancer cell proliferation, migration and invasion. Furthermore, CORO1C-regulated molecular pathways were categorized by using si-CORO1C transfectants. Further analysis of novel cancer signaling pathways modulated by the tumor-suppressive cluster miR-1/133a will provide insights into the molecular mechanisms of lung-SCC oncogenesis and metastasis.

MicroRNA-133a functions as a tumor suppressor by targeting IGF-1R in hepatocellular carcinoma

“…For example, Qiu et al [32] found that miR-133a was downregulated in gastric cancer tissues and cell lines and that overexpression of miR-133a inhibited proliferation, migration, invasion, and cell cycle progression in vitro. Mataki et al [33] reported that miR-133a was significantly downregulated in clinical lung cancer specimens and functions as tumor suppressors in lung cancer through regulation of oncogenic CORO1C. Ji et al [34] found that restoration of miR-133a expression in osteosarcoma cells significantly reduces cell proliferation, promotes cell apoptosis, and suppresses tumorigenicity via inhibiting Bcl-xl and Mcl-1.…”

Hsa-miR-1 suppresses breast cancer development by down-regulating K-ras and long non-coding RNA MALAT1

“…Depending on their specific gene or lccRNA targets, miRNAs may function as oncogenes and/or tumor suppressors [7]. MiR-1 has been reported to function as a tumor suppressor in various cancers [8][9][10]. A previous report suggests that miR-1 may be a tumor suppressor in breast cancer [11].…”

MiR-381 inhibits migration and invasion in human gastric carcinoma through downregulatedting SOX4

“…Despite of their short length, miRNAs have been confirmed to act vital important roles in various biological processes including glycometabolism (4), angiogenesis (5), cell growth (6) and movement (7).…”