Downregulation of the ubiquitin ligase KBTBD8 prevented epithelial ovarian cancer progression

Du, Lixin; Li, Congrong; He, Qifeng; Li, Xiaohua; Yang, Le; Zou, Yuan; Yang, Zhiyun; Zhang, Dong; Xing, Xiaowei

doi:10.1186/s10020-020-00226-7

Cited by 8 publications

(5 citation statements)

References 44 publications

(50 reference statements)

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“…BBK protein that has been identified as being found in the Golgi apparatus and translocating to the forming spindle after KBTBD8 is the first entry into mitosis 51 . The findings presented here indicate that KBTBD8 is also essential for the healthy function of ovarian epithelium 52 . The MLXIP interacts with Max-like protein X (MLX) to activate transcription.…”

Section: Discussionmentioning

confidence: 54%

Development and verification of a combined diagnostic model for primary Sjögren's syndrome by integrated bioinformatics analysis and machine learning

Yang

Wang

et al. 2023

Sci Rep

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Primary Sjögren’s syndrome (pSS) is a chronic, systemic autoimmune disease mostly affecting the exocrine glands. This debilitating condition is complex and specific treatments remain unavailable. There is a need for the development of novel diagnostic models for early screening. Four gene profiling datasets were downloaded from the Gene Expression Omnibus database. The ‘limma’ software package was used to identify differentially expressed genes (DEGs). A random forest-supervised classification algorithm was used to screen disease-specific genes, and three machine learning algorithms, including artificial neural networks (ANN), random forest (RF), and support vector machines (SVM), were used to build a pSS diagnostic model. The performance of the model was measured using its area under the receiver operating characteristic curve. Immune cell infiltration was investigated using the CIBERSORT algorithm. A total of 96 DEGs were identified. By utilizing a RF classifier, a set of 14 signature genes that are pivotal in transcription regulation and disease progression in pSS were identified. Through the utilization of training and testing datasets, diagnostic models for pSS were successfully designed using ANN, RF, and SVM, resulting in AUCs of 0.972, 1.00, and 0.9742, respectively. The validation set yielded AUCs of 0.766, 0.8321, and 0.8223. It was the RF model that produced the best prediction performance out of the three models tested. As a result, an early predictive model for pSS was successfully developed with high diagnostic performance, providing a valuable resource for the screening and early diagnosis of pSS.

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Section: Discussionmentioning

confidence: 54%

Development and verification of a combined diagnostic model for primary Sjögren's syndrome by integrated bioinformatics analysis and machine learning

Yang

Wang

et al. 2023

Sci Rep

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“…Five of the genes in these novel loci are involved in cardiovascular conditions and six in cancer processes. 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 In GBMI-IGGC-GGLAD meta-analysis, two GBMI novel loci, RPL37A-LINC01280 and MIR2054;INTU , maintained the genome-wide significance level ( RPL37A-LINC01280 p = 4.16e−9; MIR2054;INTU p = 1.54e−9). While the other three GBMI novel loci, CCDC13 , MYO1B;NABP1 , and ZFP91-CNTF;GLYAT , were attenuated to subgenome-wide significance level ( CCDC13 p = 6.20e−8; MYO1B;NABP1 p = 2.77e−6; ZFP91-CNTF;GLYAT p = 1.69e−6) ( Tables S3 and S7 ).…”

Section: Resultsmentioning

confidence: 98%

Novel ancestry-specific primary open-angle glaucoma loci and shared biology with vascular mechanisms and cell proliferation

Lo Faro,

Bhattacharya,

Zhou

et al. 2024

Cell Reports Medicine

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“…Human and mice tissues were fixed in 4% paraformaldehyde, embedded in paraffin, and sectioned. The immunostaining was performed according to the methods described by Du et al [ 27 ]. Primary antibodies against SUN5 (Proteintech, Wuhan, China, 1:100) and pERK1/2 (Cell Signaling Technology, Danvers, MA, USA, 1:100) were used to detect their corresponding antigens.…”

Section: Methodsmentioning

confidence: 99%

Nuclear Membrane Protein SUN5 Is Highly Expressed and Promotes Proliferation and Migration in Colorectal Cancer by Regulating the ERK Pathway

Song

Liu

et al. 2022

Cancers

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SUN5 was first identified as a nuclear envelope protein involved in spermatocyte division. We found that SUN5 was highly expressed in some cancers, but its function and mechanism in cancer development remain unclear. In the present study, we demonstrated that SUN5 was highly expressed in colorectal cancer (CRC) tissues and cells, as indicated by bioinformatics analysis, and SUN5 promoted cell proliferation and migration in vitro. Moreover, the overexpression of SUN5 upregulated phosphorylated ERK1/2 (pERK1/2), whereas the knockdown of SUN5 yielded the opposite results. PD0325901 decreased the level of pERK1/2 to inhibit cell proliferation and migration, which was partially reversed by SUN5 overexpression, indicating that drug resistance exists in patients with high SUN5 expression. The Xenograft transplantation experiment showed that SUN5 accelerated tumor formation in vivo. Furthermore, we found that SUN5 regulated the ERK pathway via Nesprin2 mediation and promoted the nuclear translocation of pERK1/2 by interacting with Nup93. Thus, these findings indicated that highly expressed SUN5 promoted CRC proliferation and migration by regulating the ERK pathway, which may contribute to the clinical diagnosis and new treatment strategies for CRC.

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Downregulation of the ubiquitin ligase KBTBD8 prevented epithelial ovarian cancer progression

Cited by 8 publications

References 44 publications

Development and verification of a combined diagnostic model for primary Sjögren's syndrome by integrated bioinformatics analysis and machine learning

Development and verification of a combined diagnostic model for primary Sjögren's syndrome by integrated bioinformatics analysis and machine learning

Novel ancestry-specific primary open-angle glaucoma loci and shared biology with vascular mechanisms and cell proliferation

Nuclear Membrane Protein SUN5 Is Highly Expressed and Promotes Proliferation and Migration in Colorectal Cancer by Regulating the ERK Pathway

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