Doxorubicin-Wrapped Zinc Oxide Nanoclusters for the Therapy of Colorectal Adenocarcinoma

Abstract

Doxorubicin (DOX)-wrapped zinc oxide nanoclusters (ZnO NCs) were developed for the therapy of colorectal cancer. DOX was coated onto the agglomerates of ZnO nanoparticles using a facile coating process. DOX-ZnO NCs with a hydrodynamic size of 170 nm, narrow size distribution, and positive zeta potential were fabricated. The aggregated shape of developed DOX-ZnO NCs was observed by transmission electron microscopy (TEM) imaging. The result of Fourier-transform infrared (FT-IR) analysis suggested the interaction between DOX and ZnO in DOX-ZnO NCs. The existence of DOX in the outer surface of DOX-ZnO NCs was further identified by X-ray powder diffractometer (XRD) and X-ray photoelectron spectroscopy (XPS) analyses. Cellular uptake efficiency and antiproliferation efficacy of developed DOX-ZnO NCs were tested in Caco-2 (human colorectal adenocarcinoma) cells. The cellular accumulated amount of DOX-ZnO NCs was 3.19-fold higher than that of free DOX (p < 0.05). The DOX-ZnO NCs group also exhibited improved antiproliferation potentials, compared with the DOX and ZnO groups, in Caco-2 cells at 0.5 and 1 μg/mL DOX concentrations. All these findings imply that developed DOX-ZnO NCs can be efficient hybrid nanoformulations for the therapy of colorectal cancers.

Possible contribution of sialic acid to the enhanced tumor targeting efficiency of nanoparticles engineered with doxorubicin

“…2A). XPS data of NP samples can reveal the types and contents of atoms located in the outer layers [42][43][44][45][46] . In case of PLGA, it is composed of only C, H, and O atoms thus peaks for C 1 s and O 1 s were shown in the profile of PLGA NPs group.…”

Colorectal cancer combination therapy using drug and gene co-delivered, targeted poly(ethylene glycol)-&epsilon;-poly(caprolactone) nanocarriers

“…The hydrodynamic size, polydispersity, and zeta potential values of NCs were measured using dynamic light scattering (DLS) and laser Doppler methods by ELS-Z (Otsuka Electronics Co., Ltd., Hirakata, Japan) according to the manufacturer’s instructions. 26 To investigate the stability of NCs in serum, NC suspension was mixed with FBS (1:1, volume:volume) and analyzed by DLS method.…”

A Review: The Uses of Various Nanoparticles in Organic Synthesis

“…Zinc oxide nanoparticles (ZnO NPs) are cost effective and relatively less toxicity, significant biocompatibility reveal their remarkable biomedical applications, such as anticancer, drug delivery, antibacterial, diabetes treatment and anti-inflammation [30][31][32][33][34][35].…”