Doxycycline reduces osteopenia in female rats

Abstract

Doxycycline, a member of the tetracycline family, is a drug used as an antibiotic (dosage of 100 mg/day) and as an anti-inflammatory drug on the dosage of 20 mg twice a day, this use has Matrix Metalloproteinases (MMP) inhibitor action. Doxycycline is a calcium chelator and therefore interferes in bone remodeling. The main objective of this study was to evaluate the action of the drug doxycycline in the control of osteopenia. Sixty three Wistars rats were divided into 9 groups with n = 7 each, as follow: the control group with doxycycline 10 mg/kg/day (C10), control with doxycycline 30 mg/kg/day (C30) and control (C), ovariectomized group with doxycycline 10 mg/kg/day (OVX10), ovariectomized with doxycycline 30 mg/kg/day (OVX30), and ovariectomized with water (OVX), sedentary group with 10 mg/kg/day (Se10), sedentary with doxycycline 30 mg/kg/day (Se30), and sedentary group with water (Se). Left femoral bone was used for bone densitometry, right femoral bone for histological analysis. The right tibia was intended for chemical quantifications, the total serum was used for cholesterol and calcium quantification. The length of the left femoral bone was measured after the densitometry analysis. Statistical analysis was performed using multivariate general linear model (ANOVA two factors with Bonferroni adjustment) and the TRAP analysis was subjected to normality test and then were subjected to nonparametric test, both with p < 0.05 significance. Statistically significant differences were found, with better results for the groups exposed to the medication (10 and 30 mg/kg/day): Se vs. Se10 and Se vs. Se30 for BMC, quantification of magnesium, amount of cancellous bone in the distal portion; OVX vs. OVX10 for BMC, BMD and calcium in serum; OVX vs. OVX10 and OVX30 for quantification in proximal and distal portion of cancellous bone; Se vs. Se30 and OVX vs. OVX30 for immunostaining for TRAP, all results with minimum of p ≤ 0.05. Doxycycline had a deleterious effect on control groups and positive action for bone organization on female rats affected by bilateral ovariectomy-induced osteopenia and sedentary lifestyle.

Oral doxycycline pharmacokinetics: Lambs in comparison with sheep

“…The milk is still present in the diet of lambs, and probably, they still had milk in their stomachs at the start of treatment because the young animals were separated from their mothers before drug administration. Although the affinity of doxycycline to bind calcium ions is much lower than the other tetracyclines (de Fig ueiredo et al, 2019), such an interaction may cause delayed absorption.…”

“…These properties of doxycycline result in the drug accumulating in fatty tissues, including the fat of the udder and ovine milk. A contribution of binding of doxycycline to calcium ions (de Fig ueiredo et al, 2019) in milk should be considered as a factor for high concentrations in milk. Excretion of the antibiotic through the milk of sheep should be taken into account when considering group treatment of sheep kept together with their offspring.…”

Combination of Doxycycline with Metronidazole Protects against Pyroptosis in Rats with Endometritis through the Modulation of TLR4/NF-κB Pathway

“…Injection of 1 mg/kg of 1 mg/mL LPS into the uterus, and intraperitoneal injection of 100 mg/kg/day doxycycline for 5 consecutive days [22]…”

“…Grouping of rats and the exact treatments given to every group are presented in Table 1 (Ref. [20][21][22]). After treatments, the remaining total 25 rats (5 rats per group) were euthanized by cervical dislocation after being anesthetized, and uterus was extracted from each rat, fixed for 24 h in 4% paraformaldehyde, dehydrated and embedded in paraffin, and cut into 8-µm-thick sections.…”

Low-level laser therapy (LLLT) improves alveolar bone healing in rats

“…Morphometric assessment, i.e., the calculation of the area and perimeter, was made using ImageJ software [23]. The TRAP and RUNX-2 analyses were made in accordance of previous studies published by the present research group [24,25].…”