DR5 is a Suitable System for Studying the Auxin Response in the Poncirus trifoliata-Xanthomonas axonopodis pv. citri Interaction

Xiao, Xuan; Li, Xiaoyan; Chen, Chunli; Guo, Wen‐Wu

doi:10.1016/j.hpj.2020.07.001

Cited by 4 publications

(1 citation statement)

References 24 publications

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“…5A). Consistently, expression of the CsGH3.1 gene was up-regulated in both iaaM#7 and iaaM#31 roots, with 6.1-fold and 1.6-fold increases, respectively, suggesting an increases in endogenous auxin levels in these transgenic lines (Xiao et al, 2020). In the iaaM + CKX transgenic roots, both iaaM and CKX genes were expressed simultaneously (Fig.…”

Section: Overexpression Of the Iaam And Ckx Genes Alter Expression Of...supporting

confidence: 63%

Root Predominant Overexpression of iaaM and CKX Genes Promotes Root Initiation and Biomass Production and Initiation in Citrus

Tang

Li²,

Zhai³

et al. 2023

Preprint

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Promoting initiation and biomass production of roots is significant for plant-based industries including using roots as bioreactors. We used two citrus genotypes, Carrizo and US-897, as model plants to test the effects of root-predominantly overexpressed the iaaM (indoleacetic acid-tryptophan monooxygenase) gene and a CKX (a cytokinin oxidase/dehydrogenase) gene. The iaaM transgenic lines exhibited markedly faster root initiation, more root numbers, and higher root biomass compared to their wild-type counterparts. The transgenic iaaM + CKXplants also exhibited similar phenotypes, albeit to a lesser extent than the iaaMplants. Molecular analysis revealed an auxin-responsive CsGH3.1 gene was up-regulated in the iaaM roots and iaaM + CKX roots, and a cytokinin-responsive gene CsARR5 gene was down-regulated in the iaaM + CKX roots. Our results demonstrate that root predominant overexpression of the iaaM or both the iaaM and CKX genes drastically enhances the initiation, growth and biomass production of roots. These results provide additional support that manipulation of auxin and cytokinin levels in roots via transgenic or gene-editing technologies may benefit production of high-value secondary metabolites using roots as bioreactors and also improve rooting of recalcitrant plant species.

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Section: Overexpression Of the Iaam And Ckx Genes Alter Expression Of...supporting

confidence: 63%

Root Predominant Overexpression of iaaM and CKX Genes Promotes Root Initiation and Biomass Production and Initiation in Citrus

Tang

Li²,

Zhai³

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

Root predominant overexpression of iaaM and CKX genes promotes root initiation and biomass production in citrus

Tang

Zhai

et al. 2023

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Promoting initiation and biomass production of roots is significant for plant-based industries including using roots as bioreactors. Two citrus genotypes, Carrizo and US-897, were used as model plants to test the effects of root-predominantly overexpressed the iaaM (indoleacetic acid-tryptophan monooxygenase) gene and a CKX (a cytokinin oxidase/dehydrogenase) gene. The iaaM transgenic lines exhibited markedly faster root initiation, more root numbers, and higher root biomass compared to their wild-type counterparts. The transgenic iaaM + CKX plants also exhibited similar phenotypes, albeit to a lesser extent than the iaaM plants. Molecular analysis revealed an auxin-responsive CsGH3.1 gene was up-regulated in the iaaM roots and iaaM + CKX roots, and a cytokinin-responsive gene CsARR5 gene was down-regulated in the iaaM + CKX roots. Our results demonstrate that root predominant overexpression of the iaaM or both the iaaM and CKX genes drastically enhances the initiation, growth and biomass production of roots. These results provide additional support that manipulation of auxin and cytokinin levels in roots via transgenic or gene-editing technologies may benefit production of high-value secondary metabolites using roots as bioreactors and also improve rooting of recalcitrant plant species.

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The role of plant growth-promoting bacteria in alleviating drought stress on pepper plants

Admassie

Woldehawariat²,

Alemu³

et al. 2022

Agricultural Water Management

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DR5 is a Suitable System for Studying the Auxin Response in the Poncirus trifoliata-Xanthomonas axonopodis pv. citri Interaction

Cited by 4 publications

References 24 publications

Root Predominant Overexpression of iaaM and CKX Genes Promotes Root Initiation and Biomass Production and Initiation in Citrus

Root Predominant Overexpression of iaaM and CKX Genes Promotes Root Initiation and Biomass Production and Initiation in Citrus

Root predominant overexpression of iaaM and CKX genes promotes root initiation and biomass production in citrus

The role of plant growth-promoting bacteria in alleviating drought stress on pepper plants

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