Draft genome of the blister beetle, Epicauta chinensis

Tian, Xing; Su, Xinxin; Li, Chenjing; Zhou, Yifei; Li, Shuying; Guo, Juanjuan; Fan, Qiqi; Lu, Shiqi; Zhang, Yalin

doi:10.1016/j.ijbiomac.2021.11.006

Cited by 6 publications

(1 citation statement)

References 84 publications

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“…However, only Hycleus cichorii Linnaeus and Hycleus phaleratus Pallas are listed in the Pharmacopoeia of the People's Republic of China and commonly used in artificial culture [20,[23][24][25]. Although four draft reference genomes of blister beetles have been published [15,26,27], only the gene sets of H. cichorii and H. phaleratus are currently available in public databases. This observation, along with the availability of correlational studies in these organisms [20,[23][24][25], prompted us to select H. cichorii and H. phaleratus as models for investigating CTD biosynthesis genes.…”

Section: Introductionmentioning

confidence: 99%

Investigation of sex expression profiles and the cantharidin biosynthesis genes in two blister beetles

Wu,

Li,

et al. 2023

PLoS ONE

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Cantharidin (CTD) is a well-established defensive toxin synthesized by blister beetles, displaying both therapeutic potential and toxicity. Among these beetles, Hycleus cichorii and Hycleus phaleratus are the two most commercially significant species due to their capacity to produce CTD in males. In this investigation, we conducted a gene expression profiling analysis of male and female individuals of these two species, utilizing the Illumina Hiseq4000 platform. We identified 7,983 expressed genes, including 2,823 differentially expressed genes (DEGs) shared by both male and female blister beetles. Nineteen genes related to CTD biosynthesis in the terpenoid backbone biosynthesis pathway were identified, including hydroxymethylglutaryl-CoA reductase (HMGR; EC:1.1.1.34), which demonstrated a significant correlation with CTD content. Furthermore, hydroxymethylglutaryl-CoA synthase (HMGS; EC:2.3.3.10) and isopentenyl-diphosphate Delta-isomerase (IDI; EC:5.3.3.2) were also found to be significantly up-regulated in males. Comparative analysis revealed that NADP+-dependent farnesol dehydrogenase (FOHSDR; EC:1.1.1.216) and farnesyl diphosphate synthase (FDPS; EC:2.5.1.1) had the highest copy number in these beetles, significantly higher than the copy number of the other four non-Meloidae insects. The analysis of the protein-protein interaction network of genes related to CTD biosynthesis revealed that the acetyl-CoA C-acetyltransferase (ACAT; EC:2.3.1.9) gene was the central gene, exhibiting greater expression in male blister beetles than in females. This study offers novel insights into the mechanisms of CTD biosynthesis in blister beetles and enhances our comprehensions of the association between particular genes and CTD content.

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Section: Introductionmentioning

confidence: 99%

Investigation of sex expression profiles and the cantharidin biosynthesis genes in two blister beetles

Wu,

Li,

et al. 2023

PLoS ONE

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The complete mitochondrial genome of Epicauta sibirica Pallas, 1773 (Coleoptera: Meloidae)

Yang,

Zhang,

Jin

et al. 2024

Mitochondrial DNA Part B

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Epicauta sibirica is both an important pest and an important economic insect. The mitogenome is 15,717 bp in length and consists of 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and a control region. The nucleotide composition of E. sibirica is 37.0% of A, 18.3% of C, 11.3% of G, and 33.4% of T. The genus Epicauta was detected as a monophyletic group and more closely related to the genus Lytta than other genera within Meloidae. This study supplemented additional genetic data for future research on the phylogeny and evolution of Meloidae species.

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The Detoxification Enzymatic Responses of Plutella xylostella (Lepidoptera: Plutellidae) to Cantharidin

Sun

Wang

Wei

et al. 2022

Journal of Economic Entomology

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Plutella xylostella (L.) (Lepidoptera: Plutellidae) is one of the most destructive pests of Brassicaceae vegetables. Cantharidin is an insect-derived defensive toxin, which has been reported to have toxicity to a variety of pests and especially lepidopteran pests. Although the toxicity of cantharidin on P. xylostella has been demonstrated, there is little information available on the specific detoxification response of P. xylostella against cantharidin. This study investigates the enzymatic response (including serine/threonine phosphatases [PSPs], carboxylesterases [CarEs], glutathione-S-transferases [GSTs], and cytochrome P450 monooxygenases [P450]) in P. xylostella to the sublethal and low lethal concentrations of cantharidin (LC10 and LC25). Results showed that the inhibitory activity of PSPs was increased and then decreased in vivo, while PSPs activity could be almost completely inhibited in vitro. Interestingly, the activities of detoxification enzymes (GST, CarE, and P450) in P. xylostella displayed a trend of decreasing and then increasing after exposure to the two concentrations of cantharidin. Notably, the increase in P450 enzyme activity was the most significant. The increasing trend of detoxification enzyme activity was congruent with the recovery trend of PSPs activity. This study contributes to our understanding of the detoxification mechanism of cantharidin in P. xylostella and helps in the further development of biogenic agents.

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Draft genome of the blister beetle, Epicauta chinensis

Cited by 6 publications

References 84 publications

Investigation of sex expression profiles and the cantharidin biosynthesis genes in two blister beetles

Investigation of sex expression profiles and the cantharidin biosynthesis genes in two blister beetles

The complete mitochondrial genome of Epicauta sibirica Pallas, 1773 (Coleoptera: Meloidae)

The Detoxification Enzymatic Responses of Plutella xylostella (Lepidoptera: Plutellidae) to Cantharidin

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