Draft genome sequence data of Paenibacillus cisolokensis strain LC2-13A and Xylanibacillus composti strain K-13

Abstract

To discover more efficient degradation processes of lignocellulosic biomass, it is still important to analyze genomic and enzymatic data from bacteria that have strong xylanolytic ability. Here, we present the draft genome sequences of the xylanolytic bacteria Paenibacillus cisolokensis strain LC2-13A and Xylanibacillus composti strain K-13 that are closest to Paenibacillus sp. strain DA-C8, which has strong xylan degradation ability under anaerobic growth conditions. Whole-genome sequencing on the Ion GeneStudio S5 System yielded 277 contigs with total size 5,305,208 bp and G+C content 52.3 mol% for strain LC2-13A and 115 contigs with total size 4,652,266 bp and G+C content of 56.2 mol% for strain K-13. The LC2-13A genome had 5,744 protein-coding sequences (CDSs), 57 tRNAs, and 4 clustered regularly interspaced short palindromic repeats (CRISPRs), and the K-13 genome had 4,388 CDSs, 1 rRNA gene, 45 tRNAs, and 5 CRISPRs. The CDSs of LC2-13A and K-13 encoded the following carbohydrate-active enzymes: 98 and 67 glycoside hydrolases, 31 and 29 glycosyl transferases, 23 and 17 carbohydrate esterases, and 13 and 37 carbohydrate-binding modules, respectively. The whole-genome sequences of LC2-13A and K-13 have been deposited in DDBJ/ENA/GenBank under accession numbers BOVK00000000 and BOVJ00000000. The versions described in this paper are version 1.

Insulambacter thermoxylanivorax sp. nov., a thermophilic xylanolytic bacterium isolated from compost

“…cisolokensis LC2-13A T was nearly identical in its G+C content (56.6 mol%), but its genome was approximately 1.7 times (5 305 208 bp) that of strain DA-C8 T [26]. Xylanibacillus composti K13, representing the closest other genus, also has a slightly bigger genome (4 652 266 bp) with a G+C content of 52.3 mol% [26]. Average nucleotide identity (ANI) [27], digital DNA–DNA hybridization (dDDH) [28] and average amino acid sequence identity (AAI) values were calculated using the ANI calculator (www.ezbiocloud.net/tools/ani) [29], the Genome-to-Genome Distance Calculator (http://ggdc.dsmz.de/distcalc2.php) and the AAI calculator (http://enve-omics.ce.gatech.edu/aai), respectively.…”

“…The whole-genome sequence was deposited in GenBank under accession number BMAQ00000000 [25]. The closely related strain P. cisolokensis LC2-13A T was nearly identical in its G+C content (56.6 mol%), but its genome was approximately 1.7 times (5 305 208 bp) that of strain DA-C8 T [26]. Xylanibacillus composti K13, representing the closest other genus, also has a slightly bigger genome (4 652 266 bp) with a G+C content of 52.3 mol% [26].…”