Draft Genome Sequence of Bacillus licheniformis S127, Isolated from a Sheep Udder Clinical Infection

Ostrov, Ievgenia; Sela, Noa; Freed, Mor; Khateb, Nihaya; Kott-Gutkowski, Miriam; Inbar, Dana; Shemesh, Moshe

doi:10.1128/genomea.00971-15

Cited by 9 publications

(10 citation statements)

References 12 publications

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“…The most common Bacillus species found in dairy associated environment are B. licheniformis , B. cereus , B . subtilis , B. mycoides and B. megaterium ( Andersson et al, 1995 ; Bartoszewicz et al, 2008 ; De Jonghe et al, 2008 ; Ledenbach and Marshall, 2009 ; Ostrov et al, 2015 ). It was previously shown that the majority of isolates from raw milk from organic and conventional dairy farms belonged to the genus Bacillus and showed at least 97% 16S rRNA gene sequence similarity with type strains of B. licheniformis , B. pumilus , B. circulans , B. subtilis and B. cereus ( Coorevits et al, 2008 ).…”

Section: Introductionmentioning

confidence: 99%

“…Strains belonging to the B. subtilis and B. cereus groups were shown to be strongly proteolytic ( Lücking et al, 2013 ). Interestingly, Bacillus strains including B. cereus ( Wijman et al, 2007 ), B. licheniformis ( Hoong et al, 2012 ; Ostrov et al, 2015 ) and B. subtilis ( Bridier et al, 2011 ) are able to form submerged surface-associated biofilm.…”

Section: Introductionmentioning

confidence: 99%

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Development of a Method to Determine the Effectiveness of Cleaning Agents in Removal of Biofilm Derived Spores in Milking System

et al. 2016

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Microbial damages caused by biofilm forming bacteria in the dairy industry are a fundamental threat to safety and quality of dairy products. In order to ensure the optimal level of equipment hygiene in the dairy industry, it is necessary to determine the biofilm removal efficiency of cleaning agents used for cleaning-in-place (CIP) procedures. However, currently there is no standard method available for evaluating and comparing cleaning agents for use in CIP procedures in the dairy industry under realistic conditions. The present study aims to establish a CIP model system to evaluate the effectiveness of cleaning agents in removal of biofilm derived spores from the surfaces of stainless steel which is the predominant substrate in milking equipment on dairy farms. The system is based on Bacillus subtilis spores surrounded with exopolymeric substances produced by bacteria during biofilm formation. The spores applied on sampling plates were mounted on T-junctions protruding 1.5–11-times the milk pipe diameter from the main loop to resemble different levels of cleaning difficulty. The cleaning tests were conducted using commercial alkaline detergents and caustic soda at conditions which are relevant to actual farm environment. The spores removal effect was evaluated by comparing the number of viable spores (attached to sampling plates) before and after cleaning. Evaluation of the cleaning and disinfecting effect of cleaning agents toward biofilm derived spores was further performed, which indicates whether spores elimination effect of an agent is due to killing the spores or removing them from the surfaces of dairy equipment. Moreover, it was established that the presence of extracellular matrix is an important factor responsible for high level of cleaning difficulty characteristic for surface attached spores. In overall, the results of this study suggest that the developed model system simulates actual farm conditions for quantitative evaluation of the effectiveness of cleaning and disinfecting agents and their cleaning and disinfecting effect on removal of biofilm derived spores.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of a Method to Determine the Effectiveness of Cleaning Agents in Removal of Biofilm Derived Spores in Milking System

et al. 2016

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“…The following bacterial strains were used in this study: (i) dairy-associated isolates, such as B. paralicheniformis S127 [24,25], B. licheniformis MS310, B. subtilis MS302, B. paralicheniformis MS303 [24]; (ii) non-dairy isolate B. subtilis NCIB3610 (descendant of B. subtilis Marburg); (iii) poly-γ-glutamic acid (PGA)-overproducing mutant derivatives of B. subtilis 3610, B. subtilis YC295 (Δ ywcC ) and B. subtilis YY54 (Δ pgdS ) (a gift of Y. Chai [18]). B. licheniformis MS310, B. subtilis MS302 and B. paralicheniformis MS303 whole-genome shotgun projects are deposited at DDBJ/EMBL/GenBank, under accession numbers MIPQ00000000, MIZD00000000, MIZE00000000 respectively.…”

Section: Methodsmentioning

confidence: 99%

Robust Biofilm-Forming Bacillus Isolates from the Dairy Environment Demonstrate an Enhanced Resistance to Cleaning-in-Place Procedures

Ostrov

Paz

Shemesh

2019

Foods

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One of the main strategies for maintaining the optimal hygiene level in dairy processing facilities is regular cleaning and disinfection, which is incorporated in the cleaning-in-place (CIP) regimes. However, a frail point of the CIP procedures is their variable efficiency in eliminating biofilm bacteria. In the present study, we evaluated the susceptibility of strong biofilm-forming dairy Bacillus isolates to industrial cleaning procedures using two differently designed model systems. According to our results, the dairy-associated Bacillus isolates demonstrate a higher resistance to CIP procedures, compared to the non-dairy strain of B. subtilis. Notably, the tested dairy isolates are highly persistent to different parameters of the CIP operations, including the turbulent flow of liquid (up to 1 log), as well as the cleaning and disinfecting effects of commercial detergents (up to 2.3 log). Moreover, our observations indicate an enhanced resistance of poly-γ-glutamic acid (PGA)-overproducing B. subtilis, which produces high amounts of proteinaceous extracellular matrix, to the CIP procedures (about 0.7 log, compared to the wild-type non-dairy strain of B. subtilis). We therefore suggest that the enhanced resistance to the CIP procedures by the dairy Bacillus isolates can be attributed to robust biofilm formation. In addition, this study underlines the importance of evaluating the efficiency of commercial cleaning agents in relation to strong biofilm-forming bacteria, which are relevant to industrial conditions. Consequently, we believe that the findings of this study can facilitate the assessment and refining of the industrial CIP procedures.

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“…Moreover, they are the predominant type of gram‐positive bacteria isolated from both raw and pasteurized milk, pre‐ and post‐pasteurization segments of dairy plants . The most common Bacillus species found in dairy‐associated environments are B. licheniformis , B. cereus , B. subtilis , B. thuringiensis , B. weihenstephanensis, B. mycoides, B. sporothermodurans , and B. megaterium …”

Section: Persistence Of Biofilm Formed By Bacillus Species In the Daimentioning

confidence: 99%

“…36,43,48 The most common Bacillus species found in dairy-associated environments are B. licheniformis, B. cereus, B. subtilis, B. thuringiensis, B. weihenstephanensis, B. mycoides, B. sporothermodurans, and B. megaterium. [49][50][51] The ability to adhere to surfaces and form biofilms enables Bacillus species to thrive in the dairy-associated environment, as it facilitates their dispersion and survivability. 28,35 Thus, milkderived Bacillus strains should be characterized by the formation of robust submerged biofilm during growth in milk.…”

Section: Persistence Of Biofilm Formed By Bacillus Species In the Daimentioning

confidence: 99%

Role of Bacillus species in biofilm persistence and emerging antibiofilm strategies in the dairy industry

Shemesh

Ostrov

2020

J Sci Food Agric

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Biofilm‐forming Bacillus species are often involved in persistent contamination and spoilage of dairy products. They therefore present a major microbiological challenge in the field of dairy food quality and safety. Due to their substantial physiological versatility, Bacillus species can survive in various parts of dairy manufacturing plants, leading to a high risk of product spoilage and potential dissemination of foodborne diseases. Furthermore, biofilm and heat‐resistant spore formation make these bacteria challenging to eliminate. Thus, some strategies have been employed to remove, prevent, or delay the formation of Bacillus biofilms in the dairy industry, but with limited success. Lack of understanding of the Bacillus biofilm structure and behavior in conditions relevant to dairy‐associated environments could partially account for this situation. The current paper reviews dairy‐associated biofilm formation by Bacillus species, with particular attention to the role of biofilm in Bacillus species adaptation and survival in a dairy processing environment. Relevant model systems are discussed for the development of novel antimicrobial approaches to improve the quality of dairy food. © 2020 Society of Chemical Industry

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Draft Genome Sequence of Bacillus licheniformis S127, Isolated from a Sheep Udder Clinical Infection

Cited by 9 publications

References 12 publications

Development of a Method to Determine the Effectiveness of Cleaning Agents in Removal of Biofilm Derived Spores in Milking System

Development of a Method to Determine the Effectiveness of Cleaning Agents in Removal of Biofilm Derived Spores in Milking System

Robust Biofilm-Forming Bacillus Isolates from the Dairy Environment Demonstrate an Enhanced Resistance to Cleaning-in-Place Procedures

Role of Bacillus species in biofilm persistence and emerging antibiofilm strategies in the dairy industry

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