Draft Genome Sequence of
            <i>Nitrincola</i>
            sp. Strain A-D6, an Arsenic-Resistant Gammaproteobacterium Isolated from a Salt Flat

Valdés, Natalia; Rivera-Araya, Javier; Bijman, Jonathan; Escudero, Lorena; Demergasso, Cecilia; Fernández, Sergio; Ferrer, Alonso; Chávez, Renato; Levicán, Gloria

doi:10.1128/genomea.01144-14

Cited by 9 publications

(11 citation statements)

References 15 publications

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“…Accordingly, HAAL-isolated strains display intrinsic high UV resistance (3)(4)(5)8,10,14,(16)(17)(18)(19) that render models for studying adaptive responses and mechanisms that underlie light sensing and UV resistance in environmental microbes. In fact, the availability of genomes of model polyextremophilic microbes (17,20) and several metagenomes of complex microbial communities in microbialites is providing a great opportunity to perform in-depth basic and applied research (4,6,19,(21)(22)(23)(24)(25)(26)(27)(28)(29).…”

Section: Introductionmentioning

confidence: 99%

Forged Under the Sun: Life and Art of Extremophiles from Andean Lakes

Albarracín

Gärtner

Farı́as

2015

Photochem & Photobiology

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High-altitude Andean lakes (HAAL) are a treasure chest for microbiological research in South America. Their indigenous microbial communities are exposed to extremely high UV irradiation and to multiple chemical extremes (Arsenic, high salt content, alkalinity). Microbes are found both, free-living or associated into microbial mats with different degrees of mineralization and lithification, including unique modern stromatolites located at 3570 m above sea level. Characterization of these polyextremophilic microbes began only recently, employing morphological and phylogenetic methods as well as high-throughput sequencing and proteomics approach. Aside from providing a general overview on microbial communities, special attention is given to various survival strategies; HAAL's microbes present a complex system of shared genetic and physiological mechanisms (UV-resistome) based on UV photoreceptors and stress sensors with their corresponding response regulators, UV avoidance and protection strategies, damage tolerance and UV damage repair. Molecular information will be provided for what is, so far the most studied HAAL molecule, a CPD-Class I photolyase from Acinetobacter Ver3 (Laguna Verde, 4400 m). This work further proposes some strategies that make an appeal for the preservation of HAAL, a highly fragile environment that offers promising and ample research possibilities.

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Section: Introductionmentioning

confidence: 99%

Forged Under the Sun: Life and Art of Extremophiles from Andean Lakes

Albarracín

Gärtner

Farı́as

2015

Photochem & Photobiology

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“…Among these metalloids, arsenic is a common element that can be found in the environment in different forms (commonly trivalent [As(III)] or pentavalent [As(IV)] form). Inorganic arsenic is the most abundant arsenic species in nature and is commonly found in different environments (Valdés et al 2014). It is generally found in soil as insoluble sulfo salts and sul des such as Arsenopyrite, Orpiment, Realgar, Lollingite, and Tennantite.…”

Section: Introductionmentioning

confidence: 99%

“…From the point of view of microorganisms; it is seen that some microorganisms can cope with arsenic toxicity by using different ways. Therefore, such microorganisms play an important role in the arsenic geocycle (Valdés et al 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Although arsenic is present in living systems at a rate of 2 mg/kg and is called 'essential toxins' because it is required in trace amounts for growth activities (Krumova et al 2008), this metalloid is highly toxic to living organisms at high concentrations and this toxicity is mainly based on its chemical diversity (Valdés et al 2014). Although As(V) and As(III) arsenic forms are the most common forms in natural environments, arsine (-III) and elemental arsenic (0) forms are also basically found in nature.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Arsenic Pollution and Anaerobic Arsenic-Metabolizing Bacteria of Lake Van, the World's Largest Soda Lake

Omeroglu

2022

Preprint

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Arsenic is responsible for water pollution in many places in the World and presents a serious health risk for people. Lake Van is in the position of the world's largest soda lake and there are no studies on seasonal arsenic pollution and arsenic resistant bacteria. So, it was aimed to determine arsenic amount of lake water and sediment, to isolate arsenic metabolizing anaerobic bacteria and their identification and determination of arsenic metabolism. Sampling was done from 7.5 meters to represent the four seasons. Metal contents were determined by using ICP-MS. Pure cultures were obtained using the hungate technique. Growth characteristics of the strains were determined at different conditions as well as at arsenate and arsenite concentrations. Molecular studies were also carried out for various resistance genes. Our results showed that Lake Van’s total arsenic amount changes seasonally. As a result of 16S rRNA sequencing, it was determined that the isolates were the members of 8 different genera had arsC resistance gene. In conclusion; for the sustainability of water resources, it is necessary to prevent chemical and microorganism-based pollution. It is thought that the arsenic resistant bacteria obtained as a result of this study will contribute to the solution of environmental arsenic pollution problems, as they are the first data and provide the necessary basic data for the bioremediation studies of arsenic from contaminated environmental habitat. At the same time, the first data that will contribute to the creation of the seasonal arsenic map of Lake Van are obtained.

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“…The microbial diversity of this salt flat was first analyzed [7], then enrichment [8], isolation and sequencing efforts [9] as well as geochemical in situ investigations wer performed [10]. In addition, the distribution of genes for the As (III) oxidation ( aioA ), As (V) detoxifying respiration ( arrA ), As detoxification ( arsC ), and As (III) extrusion ( acr3 ) was explored in Salar de Ascotán and other natural environments in Northern Chile with arsenic concentrations spanning six orders of magnitude.…”

Section: Introductionmentioning

confidence: 99%

First draft genome sequence of a strain from the genus Fusibacter isolated from Salar de Ascotán in Northern Chile

Serrano

Escudero

Tebes-Cayo

et al. 2017

Stand in Genomic Sci

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Fusibacter sp. 3D3 (ATCC BAA-2418) is an arsenate-reducing halotolerant strain within the Firmicutes phylum, isolated from the Salar de Ascotán, a hypersaline salt flat in Northern Chile. This high-Andean closed basin is an athalassohaline environment located at the bottom of a tectonic basin surrounded by mountain range, including some active volcanoes. This landscape can be an advantageous system to explore the effect of salinity on microorganisms that mediate biogeochemical reactions. Since 2000, microbial reduction of arsenic has been evidenced in the system, and the phylogenetic analysis of the original community plus the culture enrichments has revealed the predominance of Firmicutes phylum. Here, we describe the first whole draft genome sequence of an arsenic-reducing strain belonging to the Fusibacter genus showing the highest 16S rRNA gene sequence similarity (98%) with Fusibacter sp. strain Vns02. The draft genome consists of 57 contigs with 5,111,250 bp and an average G + C content of 37.6%. Out of 4780 total genes predicted, 4700 genes code for proteins and 80 genes for RNAs. Insights from the genome sequence and some microbiological features of the strain 3D3 are available under Bioproject accession PRJDB4973 and Biosample SAMD00055724. The release of the genome sequence of this strain could contribute to the understanding of the arsenic biogeochemistry in extreme environments.Electronic supplementary materialThe online version of this article (doi:10.1186/s40793-017-0252-4) contains supplementary material, which is available to authorized users.

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Draft Genome Sequence of Nitrincola sp. Strain A-D6, an Arsenic-Resistant Gammaproteobacterium Isolated from a Salt Flat

Cited by 9 publications

References 15 publications

Forged Under the Sun: Life and Art of Extremophiles from Andean Lakes

Forged Under the Sun: Life and Art of Extremophiles from Andean Lakes

Evaluation of Arsenic Pollution and Anaerobic Arsenic-Metabolizing Bacteria of Lake Van, the World's Largest Soda Lake

First draft genome sequence of a strain from the genus Fusibacter isolated from Salar de Ascotán in Northern Chile

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