Draft Genome Sequence of
            <i>Pseudoalteromonas</i>
            sp. Strain R3, a Red-Pigmented
            <scp>l</scp>
            -Amino Acid Oxidase-Producing Bacterium

Yu, Zhiliang; Zhao, Minyan; Qiu, Juanping

doi:10.1128/genomea.01339-15

Cited by 2 publications

(1 citation statement)

References 10 publications

(9 reference statements)

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“…All the primers (Table 1) were designed according to the genome sequence of Pseudoalteromonas sp. R3 (GenBank access number LJDF00000000) (Yu et al 2015b). The PCR was run on CFX Connect Real-Time System (Bio-Rad, CA, USA) using amplification system consisting of an initial 10 min denaturation at 95°C, followed by 40 cycles of 15 s denaturation at 95°C and 30 s annealing/elongation at 60°C.…”

Section: Measurement Of Relative Expression Level Of Gene Using Quantmentioning

confidence: 99%

Reactive oxygen species-scavenging system is involved in l-amino acid oxidase accumulation in Pseudoalteromonas sp. B3

Yang

Wang

et al. 2017

3 Biotech

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To date, the mechanisms underlying the flavoprotein l-amino acid oxidase (LAAO) accumulation in cells remain unclear. In this study, using LAAO-producer spp. as model organisms, we found that the cell biomass is negatively associated with LAAO accumulation, whereas the LAAO accumulation is positively associated with the reactive oxygen species (ROS)-scavenging capability. The expression levels of ROS-scavenging-associated genes were up-regulated with LAAO accumulation in cells, which is presumably due to the requirement for the removal of LAAO-induced ROS. Exogenous HO exposure experiment supported that the ROS-scavenging system is associated with LAAO accumulation in . All these observations indicate that ROS-scavenging capacity determines LAAO accumulation in bacterial cells. Our results shed a light on understanding the mechanism underlying controlling and adapting to LAAO accumulation in. Besides, our findings are critical to the improvement of heterologous expression of active LAAO in the future.

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Section: Measurement Of Relative Expression Level Of Gene Using Quantmentioning

confidence: 99%

Reactive oxygen species-scavenging system is involved in l-amino acid oxidase accumulation in Pseudoalteromonas sp. B3

Yang

Wang

et al. 2017

3 Biotech

Self Cite

View full text Add to dashboard Cite

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Identification and characterization of a LuxI/R-type quorum sensing system in Pseudoalteromonas

Mao

et al. 2019

Research in Microbiology

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Draft Genome Sequence of Pseudoalteromonas sp. Strain R3, a Red-Pigmented l -Amino Acid Oxidase-Producing Bacterium

Cited by 2 publications

References 10 publications

Reactive oxygen species-scavenging system is involved in l-amino acid oxidase accumulation in Pseudoalteromonas sp. B3

Reactive oxygen species-scavenging system is involved in l-amino acid oxidase accumulation in Pseudoalteromonas sp. B3

Identification and characterization of a LuxI/R-type quorum sensing system in Pseudoalteromonas

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