Draft Genome Sequence of Multidrug-Resistant Listeria innocua Strain UAM003-1A, Isolated from a Wild Black Bear (Ursus americanus)

Parsons, Cameron; Chen, Yi; Niedermeyer, Jeffrey; Hernandez, Kevin; Kathariou, Sophia

doi:10.1128/mra.01281-19

Cited by 4 publications

(3 citation statements)

References 12 publications

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“…In fact, the draft genome of Listeria innocua UAM003-1A, available from NCBI, is also related to highly abundant CC140 [14].…”

Section: Discussionmentioning

confidence: 99%

“…These findings will challenge risk management in the food chain, as L. innocua was often assessed in the past as non-hazardious [12]. Currently, L. innocua genome sequences are still limited [14,15]. Eleven L. innocua genome assemblies are currently available from the National Center for Biotechnology Information (NCBI) (https://www.ncbi.nlm.nih.gov/genome/genomes/1024?…”

Section: Introductionmentioning

confidence: 99%

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Predominance of Distinct Listeria Innocua and Listeria Monocytogenes in Recurrent Contamination Events at Dairy Processing Facilities

Kaszoni-Rückerl

Mustedanagic²,

Muri-Klinger

et al. 2020

Microorganisms

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The genus Listeria now comprises up to now 21 recognized species and six subspecies, with L. monocytogenes and L. innocua as the most prevalent sensu stricto associated species. Reports focusing on the challenges in Listeria detection and confirmation are available, especially from food-associated environmental samples. L. innocua is more prevalent in the food processing environment (FPE) than L. monocytogenes and has been shown to have a growth advantage in selective enrichment and agar media. Until now, the adaptive nature of L. innocua in FPEs has not been fully elucidated and potential persistence in the FPE has not been observed. Therefore, the aim of this study is to characterize L. innocua (n = 139) and L. monocytogenes (n = 81) isolated from FPEs and cheese products collected at five dairy processing facilities (A–E) at geno- and phenotypic levels. Biochemical profiling was conducted for all L. monocytogenes and the majority of L. innocua (n = 124) isolates and included a rhamnose positive reaction. L. monocytogenes isolates were most frequently confirmed as PCR-serogroups 1/2a, 3a (95%). Pulsed-field gel electrophoresis (PFGE)-typing, applying the restriction enzymes AscI, revealed 33 distinct Listeria PFGE profiles with a Simpson’s Index of Diversity of 0.75. Multi-locus sequence typing (MLST) resulted in 27 STs with seven new L. innocua local STs (ST1595 to ST1601). L. innocua ST1597 and ST603 and L. monocytogenes ST121 and ST14 were the most abundant genotypes in dairy processing facilities A–E over time. Either SSI-1 (ST14) or SSI-2 (ST121, all L. innocua) were present in successfully FPE-adapted strains. We identified housekeeping genes common in Listeria isolates and L. monocytogenes genetic lineage III. Wherever there are long-term contamination events of L. monocytogenes and other Listeria species, subtyping methods are helpful tools to identify niches of high risk.

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“…In fact, the draft genome of Listeria innocua UAM003-1A, available from NCBI, is also related to highly abundant CC140 [14].…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Predominance of Distinct Listeria Innocua and Listeria Monocytogenes in Recurrent Contamination Events at Dairy Processing Facilities

Kaszoni-Rückerl

Mustedanagic²,

Muri-Klinger

et al. 2020

Microorganisms

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show abstract

“…L. monocytogenes exhibits innate resistance to nalidixic acid, oxacillin, and certain third–generation cephalosporins but is not commonly resistant to antibiotics employed for treatment [ 9 , 10 , 11 ]. However, there are several reports of resistance to clinically-relevant antibiotics in L. monocytogenes and other Listeria species [ 10 , 12 , 13 , 14 ]. Thus, antimicrobial resistance (AMR) in this serious human and animal pathogen is of concern, in agreement with the global trend of growing AMR challenges in agriculture and the food chain [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Use of Bacteriophage Amended with CRISPR-Cas Systems to Combat Antimicrobial Resistance in the Bacterial Foodborne Pathogen Listeria monocytogenes

2021

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Listeria monocytogenes is a bacterial foodborne pathogen and the causative agent of the disease listeriosis, which though uncommon can result in severe symptoms such as meningitis, septicemia, stillbirths, and abortions and has a high case fatality rate. This pathogen can infect humans and other animals, resulting in massive health and economic impacts in the United States and globally. Listeriosis is treated with antimicrobials, typically a combination of a beta-lactam and an aminoglycoside, and L. monocytogenes has remained largely susceptible to the drugs of choice. However, there are several reports of antimicrobial resistance (AMR) in both L. monocytogenes and other Listeria species. Given the dire health outcomes associated with listeriosis, the prospect of antimicrobial-resistant L. monocytogenes is highly problematic for human and animal health. Developing effective tools for the control and elimination of L. monocytogenes, including strains with antimicrobial resistance, is of the utmost importance to prevent further dissemination of AMR in this pathogen. One tool that has shown great promise in combating antibiotic-resistant pathogens is the use of bacteriophages (phages), which are natural bacterial predators and horizontal gene transfer agents. Although native phages can be effective at killing antibiotic-resistant pathogens, limited host ranges and evolved resistance to phages can compromise their use in the efforts to mitigate the global AMR challenge. However, recent advances can allow the use of CRISPR-Cas (clustered regularly interspaced short palindromic repeats-CRISPR-associated proteins) to selectively target pathogens and their AMR determinants. Employment of CRISPR-Cas systems for phage amendment can overcome previous limitations in using phages as biocontrol and allow for the effective control of L. monocytogenes and its AMR determinants.

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Photoactivated Carbon Dots for Inactivation of Foodborne Pathogens Listeria and Salmonella

Dong

Wang

Darby

et al. 2021

Appl Environ Microbiol

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Foodborne pathogens have long been recognized as major challenges for the food industry and repeatedly implicated in food product recalls and outbreaks of foodborne diseases. This study demonstrated the application of a recently discovered class of visible-light activated carbon-based nanoparticles, namely carbon dots (CDots), for photodynamic inactivation of foodborne pathogens. The results demonstrated that CDots were highly effective in the photo-inactivation of Listeria monocytogenes in suspensions and on stainless steel surfaces. However, it was much less effective to Salmonella cells, but treatments with higher CDots concentration and longer time were still able to inactivate Salmonella cells. Mechanistic implications of the observed different antibacterial effects on the two types of cells were discussed, and the associated generation of intracellular reactive oxygen species (ROS), the resulted lipid peroxidation, and the leakage of nucleic acid and proteins from the treated cells were analyzed, with the results collectively suggesting CDots as a class of promising photodynamic inactivation agents for foodborne pathogens. Importance: Foodborne infectious diseases have long been recognized as major challenges in public health. Contaminations of food processing facilities and equipment with foodborne pathogens occur often. There is a critical need for new tools/approaches to control the pathogens and prevent such contaminations in food processing facilities and other settings. This study reports a newly established antimicrobial nanomaterials platform, carbon dots (CDots) coupled with visible/natural light, for effectively and efficiently inactivation of representative foodborne bacterial pathogens. The study will contribute to promoting the practical application of CDots as a new class of promising nanomaterial-based photodynamic inactivation agents for foodborne pathogens.

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Draft Genome Sequence of Multidrug-Resistant Listeria innocua Strain UAM003-1A, Isolated from a Wild Black Bear (Ursus americanus)

Cited by 4 publications

References 12 publications

Predominance of Distinct Listeria Innocua and Listeria Monocytogenes in Recurrent Contamination Events at Dairy Processing Facilities

Predominance of Distinct Listeria Innocua and Listeria Monocytogenes in Recurrent Contamination Events at Dairy Processing Facilities

Use of Bacteriophage Amended with CRISPR-Cas Systems to Combat Antimicrobial Resistance in the Bacterial Foodborne Pathogen Listeria monocytogenes

Photoactivated Carbon Dots for Inactivation of Foodborne Pathogens Listeria and Salmonella

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