Draft Genome Sequence of Mycobacterium bovis 04-303, a Highly Virulent Strain from Argentina

Nishibe, Christiane; Castelão, Ana Beatriz Canevari; Costa, Ricardo Dalla; Pinto, Beatriz Jeronimo; Varuzza, Leonardo; Cataldi, Angel; Bernardelli, Amelia; Bigi, Fabiana; Blanco, Federico Carlos; Zumárraga, Martín José; Almeida, Nalvo F.; Araújo, Flábio R.

doi:10.1128/genomea.00931-13

Cited by 9 publications

(4 citation statements)

References 15 publications

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“…M. bovis 04-303 (Nishibe et al, 2013) and M. bovis 534 were deposited in the DDBJ/EMBL/GenBank under the accession numbers GenBank: AVSW01000000 and JQEM00000000, respectively. The genome assembly showed 169 (average coverage of 27.3 x) and 72 contigs (average coverage of 50.5 x) for the 04-303 and 534 strains, respectively.…”

Section: Genome Sequencing and Annotationmentioning

confidence: 99%

“…The M. bovis 04-303 strain was isolated from a wild boar in the La Pampa province of Argentina in 2004, and characterized as spoligotype SB0140, with a Mycobacterial Interspersed Repetitive Units (MIRU) pattern of 232224263322 (Aguilar León et al, 2009;Nishibe et al, 2013). The M. bovis 534 strain, on the other hand was isolated from cattle in the Santa Fe province of Argentina, in 1997.…”

Section: Introductionmentioning

confidence: 99%

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Analysing nonsynonymous mutations between two Mycobacterium bovis strains with contrasting pathogenic profiles

Bigi

Vázquez

Castelão

et al. 2019

Veterinary Microbiology

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Section: Genome Sequencing and Annotationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Analysing nonsynonymous mutations between two Mycobacterium bovis strains with contrasting pathogenic profiles

Bigi

Vázquez

Castelão

et al. 2019

Veterinary Microbiology

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“…Another group of six animals was infected with a wild boar virulent strain M. bovis 04-303 by intratracheal instillation of 5 × 10 7 CFU as described previously [ 27 ]. The strain M. bovis 04-303 is an isolate obtained from a wild boar with tuberculous lesions [ 28 ]. Briefly, this inoculation procedure was carried out by anaesthetizing the calves with xylazine HCl (Rompun, Bayer, Germany; 0.1 mg/kg) intravenously and then inserting an 80 cm endotracheal tube into the trachea.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of Cocktails with Recombinant Proteins ofMycobacterium bovisfor a Specific Diagnosis of Bovine Tuberculosis

Mon¹,

Moyano²,

Viale³

et al. 2014

BioMed Research International

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The Delayed type hypersensitivity skin test (DTH) and interferon-gamma assay are used for the diagnosis of bovine tuberculosis (TBB). The specificity of these diagnoses, however, is compromised because both are based on the response against purified protein derivative of Mycobacterium bovis (PPD-B). In this study, we assessed the potential of two cocktails containing M. bovis recombinant proteins: cocktail 1 (C1): ESAT-6, CFP-10 and MPB83 and cocktail 2 (C2): ESAT-6, CFP-10, MPB83, HspX, TB10.3, and MPB70. C1, C2, and PPD-B showed similar response by DTH in M. bovis-sensitized guinea pigs. Importantly, C1 induced a lower response than PPD-B in M. avium-sensitized guinea pigs. In cattle, C1 displayed better performance than PPD-B and C2; indeed, C1 showed the least detection of animals either vaccinated or Map-infected. To optimize the composition of the cocktails, we obtained protein fractions from PPD-B and tested their immunogenicity in experimentally M. bovis-infected cattle. In one highly reactive fraction, seven proteins were identified. The inclusion of FixB in C1 enhanced the recognition of M. bovis-infected cattle without compromising specificity. Our data provide a promising basis for the future development of a cocktail for TBB detection without interference by the presence of sensitized or infected animals with other mycobacteria.

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“…This fact suggests that the Brazilian M. bovis AN5 isolate might be missing some PPD genes or that the genes contain a high degree of variation. Genes coding for all 116 proteins found in both PPDs were compared to their orthologs in M. bovis AF2122/97 ( 1 ), M. bovis 04-303 ( 9 ), and M. tuberculosis H37Rv ( 10 ) and showed a high degree of conservation, with identities ranging from 98% to 100%. In addition, no frameshifts were detected.…”

Section: Genome Announcementmentioning

confidence: 99%

Draft Genome Sequence of Mycobacterium bovis Strain AN5, Used for Production of Purified Protein Derivative

et al. 2014

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Draft Genome Sequence of Mycobacterium bovis 04-303, a Highly Virulent Strain from Argentina

Cited by 9 publications

References 15 publications

Analysing nonsynonymous mutations between two Mycobacterium bovis strains with contrasting pathogenic profiles

Analysing nonsynonymous mutations between two Mycobacterium bovis strains with contrasting pathogenic profiles

Evaluation of Cocktails with Recombinant Proteins ofMycobacterium bovisfor a Specific Diagnosis of Bovine Tuberculosis

Draft Genome Sequence of Mycobacterium bovis Strain AN5, Used for Production of Purified Protein Derivative

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