Draft Genome Sequence of Mycoplasma wenyonii, a Second Hemotropic Mycoplasma Species Identified in Mexican Bovine Cattle

Quiroz-Castañeda, Rosa Estela; Martínez-Ocampo, Fernando; Dantán-González, Edgar

doi:10.1128/mra.00875-18

Cited by 9 publications

(11 citation statements)

References 15 publications

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“…Currently, 12 hemoplasma genomes have been identified in the GenBank database ( , accessed on 15 August 2022), including Mycoplasma (M.) wenyonii strains: Massachusetts and INIFAP02, and one Candidatus ( Ca.) Mycoplasma haemobos strain, INIFAP01 [ 5 , 6 , 7 ]. These hemoplasma genomes provided relevant information about possible pathogenic mechanisms, metabolism, and divergence compared to other mycoplasma species [ 1 ].…”

Section: Introductionmentioning

confidence: 99%

An Update of Bovine Hemoplasmas Based on Phylogenetic and Genomics Analysis

et al. 2022

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Mycoplasma wenyonii and ‘Candidatus Mycoplasma haemobos’ are bacteria that have been described as significant hemoplasmas that infect cattle worldwide. Currently, three bovine hemoplasma genomes are known. This work aimed to describe the main genomic characteristics and the evolutionary relationships between hemoplasmas, and provide a list of epitopes predicted by immunoinformatics as diagnostic candidates for bovine hemoplasmosis. Thus far, there is no vaccine to prevent this disease that economically impacts cattle production worldwide. Additionally, there is a lack of vaccines against bovine hemoplasmosis. In this work, we performed a genomic characterization of hemoplasmas, including two Mexican strains reported in bovines in the last few years. The generated information is a new scenario about the phylogeny of hemoplasmas. Also, we show genomic features among hemoplasmas that strengthen their characteristic genome plasticity of intracellular lifestyles. Finally, the elucidation of antigenic proteins in Mexican strains represents an opportunity to develop molecular detection methods and diagnoses.

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Section: Introductionmentioning

confidence: 99%

An Update of Bovine Hemoplasmas Based on Phylogenetic and Genomics Analysis

et al. 2022

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“…Hemoplasmas are transmitted by blood-feeding arthropods and by direct contact. Hemoplasmas affect animal health alone or concomitantly with other microorganisms [ 9 ]. Thermoform, a cattle disease, is characterized by transient fever, anemia, anorexia, depression, edema, lymphadenopathy, decreased milk production, infertility, and reproductive inefficiency [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

First study on microscopic and molecular evidences of two bovine hemoplasma species in cattle herds in Al-Qadisiyah Province, Iraq

2022

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Background and Aim: Hemotropic Mycoplasmas are small epierythrocytic bacteria that cause infectious anemia in several livestock species and in humans. Several reports have been made on hemoplasma infections in the south and north of Iraq, but there have been no studies in the middle Euphrates of Iraq. This study aimed to evaluate the presence of hemoplasma species in cattle in Al-Qadisiyah Province, Iraq. Materials and Methods: Two hundred blood samples were collected from cattle with pale mucous membrane from regions with heavy tick endemicity. The samples were analyzed for the presence of Rickettsia pathogens using thin blood smears and the Diff-Quik stains. All the samples were also examined using polymerase chain reaction (PCR) to amplify the 16S ribosomal ribonucleic acid (rRNA) gene to confirm the presence of the smear-identified microorganisms. Ten PCR positive samples were subjected to 16S rRNA partial gene sequencing to identify the species. Results: The findings uncovered positivity in 68 (34%) blood smears. PCR revealed positive confirmation in 18 (9%) of the 200 blood samples. Mycoplasma wenyonii and Candidatus mycoplasma hemobos were identified from 10 PCR positive samples. The nucleotide sequences of the isolates were closely related to isolates from cattle, buffalo, and dogs in Vietnam, Cuba, India, and Germany. Conclusion: Bovine hemoplasma infections are prevalent in cattle in the Al-Qadisiyah Province in Iraq. Our results may have significance for the development of control programs.

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“…Recently, the Next-Generation Sequencing (NGS) in veterinary medicine has revealed the potential to design primary diagnostic, control, and prevention methods [ 11 ]. The genomes of A. marginale and R. microplus have been sequenced and published [ 12 , 13 , 14 ], and many detailed studies have been performed with the Major Surface Proteins (MSPs) of A. marginale, whether as vaccine prospects or diagnostic targets. In the first instance, individual or conglomerate proteins have not been very successful inducing protective solid immunity; as diagnostic targets, a recombinant Msp5 has been successfully used for molecular detection and the serological ELISA test [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Immunoinformatic Analysis to Identify Proteins to Be Used as Potential Targets to Control Bovine Anaplasmosis

Rodríguez-Camarillo

Quiroz-Castañeda

Aguilar-Díaz

et al. 2020

International Journal of Microbiology

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Omics sciences and new technologies to sequence full genomes provide valuable data that are revealed only after detailed bioinformatic analysis is performed. In this work, we analyzed the genomes of seven Mexican Anaplasma marginale strains and the data from a transcriptome analysis of the tick Rhipicephalus microplus. The aim of this analysis was to identify protein sequences with predicted features to be used as potential targets to control the bacteria or tick-vector transmission. We chose three amino acid sequences different to all proteins previously reported in A. marginale that have been used as potential vaccine candidates, and also, we report, for the first time, the presence of a peroxinectin protein sequence in the transcriptome of R. microplus, a protein associated with the immune response of ticks. The bioinformatics analyses revealed the presence of B-cell epitopes in all the amino acid sequences chosen, which opens the way for their likely use as single or arranged peptides to develop new strategies for the control and prevention of bovine anaplasmosis transmitted by ticks.

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Draft Genome Sequence of Mycoplasma wenyonii, a Second Hemotropic Mycoplasma Species Identified in Mexican Bovine Cattle

Abstract: The hemotropic mycoplasma (hemoplasma) Mycoplasma wenyonii is an animal pathogen that affects bovine cattle health. Here, we present the draft genome sequence of the hemoplasma M. wenyonii strain INIFAP02 found in cattle from Mexico.

Cited by 9 publications

References 15 publications

An Update of Bovine Hemoplasmas Based on Phylogenetic and Genomics Analysis

An Update of Bovine Hemoplasmas Based on Phylogenetic and Genomics Analysis

First study on microscopic and molecular evidences of two bovine hemoplasma species in cattle herds in Al-Qadisiyah Province, Iraq

Immunoinformatic Analysis to Identify Proteins to Be Used as Potential Targets to Control Bovine Anaplasmosis

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