Draft Genome Sequence of the Entomopathogenic Bacterium Bacillus pumilus 15.1, a Strain Highly Toxic to the Mediterranean Fruit Fly
            <i>Ceratitis capitata</i>

Garcia-Ramon, Diana C.; Palma, Leopoldo; Berry, Colin; Osuna, Antonio; Vı́lchez, Susana

doi:10.1128/genomea.01019-15

Cited by 2 publications

(8 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The enriched crystal proteins from both bands produced a major band of 45 kDa on SDS‐PAGE, as previously observed (Garcia‐Ramon et al ., ), and this was excised for fingerprint analysis using MALDI‐TOF MS. Mass spectrometry of the intact protein revealed a mass of 43 799 Da. Comparison of mass peaks obtained from fingerprinting with the recently published B. pumilus 15.1 genome (Garcia‐Ramon et al ., ) and Bacillus databases produced matches (40.8% sequence coverage) with OxdD, a putative oxalate decarboxylase encoded in Contig 4 of the B. pumilus 15.1 strain genome and an OxdD from B. pumilus ATCC 7061. The predicted MW of this B. pumilus 15.1 OxdD protein was 43 799.1 Da, corresponding to the molecular weight determined by MS.…”

Section: Resultsmentioning

confidence: 99%

“…We can also conclude that it is highly probable that the gene encoding the 17 kDa BslA‐like protein is present in the megaplamid pBp15.1B as the protein does not express in the cured strain. To prove this, two primers based on the gene yuaB in the strain 15.1 genome (Garcia‐Ramon et al ., ) were designed. A 727 bp product was detected only when DNA from the wild‐type strain was used as template, but not when total DNA from B. pumilus 15.1C was used (data not shown).…”

Section: Resultsmentioning

confidence: 99%

“…This has been demonstrated for Burkholderia glumae , a plant pathogen that causes seedling and grain rot via the production of oxalate (Li et al ., ). Although we cannot state definitively whether strain 15.1 is able to produce oxalate, the genome data for this strain (Garcia‐Ramon et al ., ) do not appear to exhibit genes encoding ascorbate 2,3 dioxygenase (which can produce oxalate from L‐ascorbate), (S)‐hydroxyl acid dehydrogenase (which can produce oxalate from glyoxalate) or oxalate CoA transferase and glyoxylate dehydrogenase (which together can produce oxalate from glyoxylate via oxalylCoA). The genome does, however, encode a putative oxalate:formate symporter in the MSF family, which is present in other B. pumilus genomes and is conserved in other bacilli but is found in few species outside this genus, so we could speculate that the strain could utilize oxalate from the medium and use oxalate decarboxylase to produce formate as a virulence factor.…”

Section: Discussionmentioning

confidence: 99%

“…The sequence obtained was compared with protein sequences from the genome of B. pumilus 15.1 (GenBank ; Garcia‐Ramon et al ., ).…”

Section: Methodsmentioning

confidence: 97%

“…Digestion products were analysed by MALDI MS (4700 Proteomics analyser of the Applied Biosystems, Foster City, California, USA). Searches of the B. pumilus 15.1 genome (Garcia‐Ramon et al ., ) and public databases were performed using MASCOT search engine (Matrix‐Science, London, UK). The services from ‘SCSIE University of Valencia Proteomics Unit’ and ‘CBMSO Protein Chemistry Facility’ that belong to the ProteoRed Proteomics Platform were also used.…”

Section: Methodsmentioning

confidence: 99%

See 4 more Smart Citations

The parasporal crystals of Bacillus pumilus strain 15.1: a potential virulence factor?

Garcia-Ramon

Berry

Tse

et al. 2017

Microbial Biotechnology

Self Cite

View full text Add to dashboard Cite

Summary Bacillus pumilus strain 15.1 was previously found to cause larval mortality in the Med‐fly Ceratitis capitata and was shown to produce crystals in association with the spore. As parasporal crystals are well‐known as invertebrate‐active toxins in entomopathogenic bacteria such as Bacillus thuringiensis (Cry and Cyt toxins) and Lysinibacillus sphaericus (Bin and Cry toxins), the B. pumilus crystals were characterized. The crystals were composed of a 45 kDa protein that was identified as an oxalate decarboxylase by peptide mass fingerprinting, N‐terminal sequencing and by comparison with the genome sequence of strain 15.1. Synthesis of crystals by a plasmid‐cured derivative of strain 15.1 (produced using a novel curing strategy), demonstrated that the oxalate decarboxylase was encoded chromosomally. Crystals spontaneously solubilized when kept at low temperatures, and the protein produced was resistant to trypsin treatment. The insoluble crystals produced by B. pumilus 15.1 did not show significant toxicity when bioassayed against C. capitata larvae, but once the OxdD protein was solubilized, an increase of toxicity was observed. We also demonstrate that the OxdD present in the crystals has oxalate decarboxylate activity as the formation of formate was detected, which suggests a possible mechanism for B. pumilus 15.1 activity. To our knowledge, the characterization of the B. pumilus crystals as oxalate decarboxylase is the first report of the natural production of parasporal inclusions of an enzyme.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…The sequence obtained was compared with protein sequences from the genome of B. pumilus 15.1 (GenBank ; Garcia‐Ramon et al ., ).…”

Section: Methodsmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

The parasporal crystals of Bacillus pumilus strain 15.1: a potential virulence factor?

Garcia-Ramon

Berry

Tse

et al. 2017

Microbial Biotechnology

Self Cite

View full text Add to dashboard Cite

show abstract

Bacillus pumilus 15.1, a Strain Active against Ceratitis capitata, Contains a Novel Phage and a Phage-Related Particle with Bacteriocin Activity

Fernández-Fernández

Osuna

Vı́lchez

2021

IJMS

Self Cite

View full text Add to dashboard Cite

A 98.1 Kb genomic region from B. pumilus 15.1, a strain isolated as an entomopathogen toward C. capitata, the Mediterranean fruit fly, has been characterised in search of potential virulence factors. The 98.1 Kb region shows a high number of phage-related protein-coding ORFs. Two regions with different phylogenetic origins, one with 28.7 Kb in size, highly conserved in Bacillus strains, and one with 60.2 Kb in size, scarcely found in Bacillus genomes are differentiated. The content of each region is thoroughly characterised using comparative studies. This study demonstrates that these two regions are responsible for the production, after mitomycin induction, of a phage-like particle that packages DNA from the host bacterium and a novel phage for B. pumilus, respectively. Both the phage-like particles and the novel phage are observed and characterised by TEM, and some of their structural proteins are identified by protein fingerprinting. In addition, it is found that the phage-like particle shows bacteriocin activity toward other B. pumilus strains. The effect of the phage-like particles and the phage in the toxicity of the strain toward C. capitata is also evaluated.

show abstract

Draft Genome Sequence of the Entomopathogenic Bacterium Bacillus pumilus 15.1, a Strain Highly Toxic to the Mediterranean Fruit Fly Ceratitis capitata

Cited by 2 publications

References 10 publications

The parasporal crystals of Bacillus pumilus strain 15.1: a potential virulence factor?

The parasporal crystals of Bacillus pumilus strain 15.1: a potential virulence factor?

Bacillus pumilus 15.1, a Strain Active against Ceratitis capitata, Contains a Novel Phage and a Phage-Related Particle with Bacteriocin Activity

Contact Info

Product

Resources

About