Draft Genome Sequences of Four Bacterial Strains Isolated from Sediment of the South China Sea

Ishaq, Sidra Erum; Ahmad, Tariq; Hou, Jialin; Liang, Lewen; Wang, Yinzhao; Wang, Fengping

doi:10.1128/mra.00191-22

Cited by 2 publications

(1 citation statement)

References 14 publications

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“…In brief, 100 µl inoculum from dilutions of 10 −6 and 10 −7 were spread on modified Reasoner's 2A (R2A) agar medium. The inoculated agar plates were incubated aerobically at 25 °C [10,11].…”

Section: Isolation and Ecologymentioning

confidence: 99%

Mariluticola halotolerans gen. nov., sp. nov., a novel member of the family Devosiaceae isolated from South China Sea sediment

Ishaq

Ahmad

Liang

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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A novel member of class Alphaproteobacteria was isolated from marine sediment of the South China Sea. Cells of strain LMO-2T were Gram-stain negative, greyish in colour, motile, with a single lateral flagellum and short rod in shape with a slight curve. Strain LMO-2T was positive for oxidase and negative for catalase. The bacterium grew aerobically at 10–40 °C (optimum, 25–30 °C), pH 5.5–10.0 (optimum, pH 7.0) and 0–9 % NaCl (w/v; optimum, 2–3 %). Phylogenetic analysis of the 16S rRNA gene sequence and phylogenomic analysis of the whole genome sequence indicated that strain LMO-2T represents a new genus and a new species within the family Devosiaceae , class Alphaproteobacteria , phylum Pseudomonadota . Comparisons of the 16S rRNA gene sequences of strain LMO-2T showed 94.8 % similarity to its closest relative. The genome size is ~3.45 Mbp with a DNA G+C content of 58.17 mol%. The strain possesses potential capability for the degradation of complex organic matter, i.e. fatty acid and benzoate. The predominant cellular fatty acids (>10 %) were C16 : 0 and C18 : 1 ω7c 11-methyl. The sole respiratory quinone was ubiquinone-10. The major identified polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phospholipid. Based on the polyphasic taxonomic data, strain LMO-2T represents a novel genus and a novel species for which the name Mariluticola halotolerans gen. nov., sp. nov., was proposed in the family Devosiaceae . The type strain is LMO-2T (=CGMCC 1.19273T=JCM 34934T).

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Section: Isolation and Ecologymentioning

confidence: 99%