Draft Genome Sequences of Six Enterococcus faecalis Strains Isolated from Malaysian Clinical and Environmental Origins

Daniel, Diane; Gan, Han Ming; Lee, Sui Mae; Dykes, Gary A.; Rahman, Sadequr

doi:10.1128/genomea.00553-17

Cited by 2 publications

(2 citation statements)

References 9 publications

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“…The frequency of Van genes in this study was much lower than the results obtained by two other studies (0.1% and 2.9%) in Peninsular Malaysia (Ibrahim et al, 2011;Mohamed et al, 2015). However, Weng et al (2013) and Daniel et al (2017) reported no VRE isolates in their Malaysian clinical isolates. Worldwide, the prevalence of antibiotic-resistant enterococcal infections is rising (Daniel et al, 2017;Shiadeh et al, 2019).…”

Section: Van Gene Frequencies and Multidrug Resistant Phenotypementioning

confidence: 98%

“…However, Weng et al (2013) and Daniel et al (2017) reported no VRE isolates in their Malaysian clinical isolates. Worldwide, the prevalence of antibiotic-resistant enterococcal infections is rising (Daniel et al, 2017;Shiadeh et al, 2019). Several studies reported that the VanA genotype was of serious concern due to the risk of transmission to other organisms and it was more common than VanB (Azzam et al, 2023).…”

Section: Van Gene Frequencies and Multidrug Resistant Phenotypementioning

confidence: 98%

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Enterococcal species distribution, antibiotic susceptibility and Van gene frequency among patients at a tertiary hospital in Sabah

Mastor, N. N. I.,

Subbiah, V. K.,

Bakar, W. N. W. A.

et al. 2023

MJM

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Aims: Enterococcus bacteria, including some strains that are resistant to antibiotics like vancomycin can pose a threat to public health. The purpose of this study is to identify the species, antibiotic susceptibility profile and VanA/VanB gene frequencies in Enterococci isolated from patients at a tertiary hospital in Kota Kinabalu, Sabah. Methodology and results: Various bodily fluid specimens were collected from 162 patients between July 2019 and June 2021. Species confirmation and susceptibility testing were performed using an automated system. Subsequently, PCR was used to determine the presence of VanA and VanB genes. Species identification revealed the presence of five enterococcal species, namely E. faecalis (91), E. faecium (64), E. gallinarum (3) E. casseliflavus (2), along with one isolate each of E. hirae and E. avium. Overall, resistance to antibiotics like ampicillin, quinolones, tetracycline, gentamicin-syn, nitrofurantoin, glycopeptides and linezolid was generally low (<50%). However, a significant number of isolates displayed high resistance to erythromycin (>50% of samples), while resistance to tetracycline was more moderate. The frequencies of VanA and VanB genes were low (0.6 and 0%, respectively) and they were only detected in E. faecium. Conclusion, significance and impact of study:The results indicate that while the prevalence of vancomycin-resistant enterococci (VRE) may be low, there is an increasing incidence of multidrug-resistant enterococci, particularly with regards to erythromycin.

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Section: Van Gene Frequencies and Multidrug Resistant Phenotypementioning

confidence: 98%

Section: Van Gene Frequencies and Multidrug Resistant Phenotypementioning

confidence: 98%

Enterococcal species distribution, antibiotic susceptibility and Van gene frequency among patients at a tertiary hospital in Sabah

Mastor, N. N. I.,

Subbiah, V. K.,

Bakar, W. N. W. A.

et al. 2023

MJM

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Next-Generation Sequencing to Elucidate Adaptive Stress Response and Plantaricin Genes among Lactobacillus Plantarum Strains

et al. 2020

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Aim: The objective of this study was to identify the genes involved in plantaricin synthesis and adaptive stress response in four Lactobacillus plantarum strains (AS-6, AS-8, AS-9 and AS-10) and one Lactobacillus paraplantarum strain (AS-7) for their usage in medicine and industry. Materials & methods: Whole genomes of these strains were sequenced by a high-throughput sequencing technique known as next-generation sequencing via Ilumina MiSeq platform and the genes were identified by using various bioinformatics tools and software. Results: Plantaricin genes ( plnD, plnE, plnF, plnG, plnI) and genes regulating response to temperature, pH, bile salt, osmotic and oxidative stress were identified in all strains. Conclusion: Lactobacilli could be an option to combat antimicrobial resistance and might replace harmful antibiotics in future.

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Draft Genome Sequences of Six Enterococcus faecalis Strains Isolated from Malaysian Clinical and Environmental Origins

Cited by 2 publications

References 9 publications

Enterococcal species distribution, antibiotic susceptibility and Van gene frequency among patients at a tertiary hospital in Sabah

Enterococcal species distribution, antibiotic susceptibility and Van gene frequency among patients at a tertiary hospital in Sabah

Next-Generation Sequencing to Elucidate Adaptive Stress Response and Plantaricin Genes among Lactobacillus Plantarum Strains

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