Draft Genome Sequences of the Nitrate-Dependent Iron-Oxidizing Proteobacteria
            <i>Acidovorax</i>
            sp. Strain BoFeN1 and Paracoccus pantotrophus Strain KS1

Price, Alex; Macey, Michael C.; Miot, Jennyfer; Olsson-Francis, Karen

doi:10.1128/mra.01050-18

Cited by 14 publications

(14 citation statements)

References 16 publications

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“…Hence, the vivianite mineral surfaces themselves seem to catalyse the abiotic reaction between NO − 2 and Fe(II)/Fe 2+ (the stimulation of Fe-dependent nitrite reduction may also be attributed, in part, to vivianite dissolution providing ample Fe(II) substrate). Previous studies have reported on mineral-enhanced chemodenitrification (Dhakal et al, 2013;Grabb et al, 2017;Klueglein and Kappler, 2013;Rakshit et al, 2008), and the catalytic effect may be due to NO − 2 adsorption onto the minerals' surface possibly facilitating a direct electron transfer. Similar findings have previously been reported on Fe(II) oxidation promoted by electron transfer during adsorption onto an Fe(III) minerals' surface (Gorski and Scherer, 2011;Piasecki et al, 2019).…”

Section: Surface Catalysis Of Chemodenitrificationmentioning

confidence: 98%

“…One redox pair that has been proposed to be exploited by microbes under anoxic conditions through a mechanism known as nitrate-dependent Fe(II) oxidation (NDFeO) is NO − 3 /Fe 2+ (Ilbert and Bonnefoy, 2013;Straub et al, 1996). To date, genetic evidence that clearly supports this metabolic capacity of the studied microorganisms remains lacking (Price et al, 2018), and biogeochemical evidence is rare and putative. The latter is mostly based on experiments with the chemolithoautotrophic culture KS, which is a consortium of four different strains, including a relative of the microaerophilic Sideroxydans/Gallionella.…”

Section: Introductionmentioning

confidence: 99%

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Impact of reactive surfaces on the abiotic reaction between nitrite and ferrous iron and associated nitrogen and oxygen isotope dynamics

et al. 2020

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Abstract. Anaerobic nitrate-dependent Fe(II) oxidation (NDFeO) is widespread in various aquatic environments and plays a major role in iron and nitrogen redox dynamics. However, evidence for truly enzymatic, autotrophic NDFeO remains limited, with alternative explanations involving the coupling of heterotrophic denitrification with the abiotic oxidation of structurally bound or aqueous Fe(II) by reactive intermediate nitrogen (N) species (chemodenitrification). The extent to which chemodenitrification is caused (or enhanced) by ex vivo surface catalytic effects has not been directly tested to date. To determine whether the presence of either an Fe(II)-bearing mineral or dead biomass (DB) catalyses chemodenitrification, two different sets of anoxic batch experiments were conducted: 2 mM Fe(II) was added to a low-phosphate medium, resulting in the precipitation of vivianite (Fe3(PO4)2), to which 2 mM nitrite (NO2-) was later added, with or without an autoclaved cell suspension (∼1.96×108 cells mL−1) of Shewanella oneidensis MR-1. Concentrations of nitrite (NO2-), nitrous oxide (N2O), and iron (Fe2+, Fetot) were monitored over time in both set-ups to assess the impact of Fe(II) minerals and/or DB as catalysts of chemodenitrification. In addition, the natural-abundance isotope ratios of NO2- and N2O (δ15N and δ18O) were analysed to constrain the associated isotope effects. Up to 90 % of the Fe(II) was oxidized in the presence of DB, whereas only ∼65 % of the Fe(II) was oxidized under mineral-only conditions, suggesting an overall lower reactivity of the mineral-only set-up. Similarly, the average NO2- reduction rate in the mineral-only experiments (0.004±0.003 mmol L−1 d−1) was much lower than in the experiments with both mineral and DB (0.053±0.013 mmol L−1 d−1), as was N2O production (204.02±60.29 nmol L−1 d−1). The N2O yield per mole NO2- reduced was higher in the mineral-only set-ups (4 %) than in the experiments with DB (1 %), suggesting the catalysis-dependent differential formation of NO. N-NO2- isotope ratio measurements indicated a clear difference between both experimental conditions: in contrast to the marked 15N isotope enrichment during active NO2- reduction (15εNO2=+10.3 ‰) observed in the presence of DB, NO2- loss in the mineral-only experiments exhibited only a small N isotope effect (<+1 ‰). The NO2--O isotope effect was very low in both set-ups (18εNO2 <1 ‰), which was most likely due to substantial O isotope exchange with ambient water. Moreover, under low-turnover conditions (i.e. in the mineral-only experiments as well as initially in experiments with DB), the observed NO2- isotope systematics suggest, transiently, a small inverse isotope effect (i.e. decreasing NO2- δ15N and δ18O with decreasing concentrations), which was possibly related to transitory surface complexation mechanisms. Site preference (SP) of the 15N isotopes in the linear N2O molecule for both set-ups ranged between 0 ‰ and 14 ‰, which was notably lower than the values previously reported for chemodenitrification. Our results imply that chemodenitrification is dependent on the available reactive surfaces and that the NO2- (rather than the N2O) isotope signatures may be useful for distinguishing between chemodenitrification catalysed by minerals, chemodenitrification catalysed by dead microbial biomass, and possibly true enzymatic NDFeO.

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Section: Surface Catalysis Of Chemodenitrificationmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Impact of reactive surfaces on the abiotic reaction between nitrite and ferrous iron and associated nitrogen and oxygen isotope dynamics

et al. 2020

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“…Under all treatments, Acidovorax and Chlorobium were observed as dominant IOB. Members of Acidovorax, such as strains BoFeN1 and 2AN, are neutrophilic, nitrate (NO 3 − )-reducing, and Fe 2+ -oxidizing bacteria in anaerobic environments [41,42]. Therefore, NO 3 − reduction would also play a role in Fe oxidation in the simulated lake system.…”

Section: Key Taxa and Predicted Functions Of Bacteria Associated With...mentioning

confidence: 99%

Interactions of Vallisneria natans and Iron-Oxidizing Bacteria Enhance Iron-Bound Phosphorus Formation in Eutrophic Lake Sediments

et al. 2022

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Submerged macrophyte restoration and in situ phosphorus (P) passivation are effective methods for the control of internal P loading from sediments. This study explored the synergistic effects of Vallisneria natans and iron (Fe)-oxidizing bacteria (IOB) on internal P loading from eutrophic freshwater lake sediments by taking into account Fe-bound P (FeP) formation and associated bacterial community structures. Sediment samples were prepared in glass tanks under four treatments, namely no V. natans planting or IOB inoculation (control), planting V. natans without IOB inoculation (Va), planting V. natans with IOB inoculation (Va-IOB), and planting V. natans with autoclaved IOB inoculation (Va-IOB[A]). Compared with the control, all three treatments with V. natans (Va, Va-IOB, and Va-IOB[A]) had significantly decreased organic matter contents and increased redox potential in sediments (p < 0.05), at the rapid growth and mature stages of V. natans. Planting V. natans with and without IOB inoculation also decreased the total P (TP) and Fe–P concentrations in sediments. Conversely, Fe3+ concentrations, Fe3+/Fe2+ ratios, and the proportions of Fe–P in TP all increased in sediments planted with V. natans, especially under the Va-IOB treatment (p < 0.05). Furthermore, bacterial community diversity increased in sediments due to the presence of V. natans. The relative abundances of IOB (including Acidovorax and Chlorobium) increased from the transplanting to the rapid growth stage of V. natans and then decreased afterwards. In the later stages, the relative abundances of IOB and their ratios to Fe-reducing bacteria were the highest under the Va-IOB treatment. Accordingly, synergistic interactions between V. natans and IOB could enhance Fe–P formation and reduce TP concentrations in eutrophic lake sediments by altering sediment physicochemical properties and Fe oxidation-related bacterial community structures.

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“…12 In addition, some heterotrophic denitrifying bacteria are able to catalyze Fe(II) oxidation, in a process known as nitrate-dependent iron(II) oxidation (NDFO), which is thought to be a result of both biotic and indirect biotic effects, 13 although, the mechanism of Fe(II) oxidation in these heterotrophs is not fully resolved, and no enzymatic Fe(II) oxidation has been reported to date. 14,15 Moreover, nitrite, or most likely its selfdecomposition products, NO and NO 2 can abiotically oxidize Fe(II). 16 These reactive nitrogen species are also intermediary compounds produced and accumulated during denitrification, 17 and have been shown to be responsible for most of the Fe(II) oxidation observed.…”

Section: Introductionmentioning

confidence: 99%

Elucidating heterogeneous iron biomineralization patterns in a denitrifying As(iii)-oxidizing bacterium: implications for arsenic immobilization

Lopez-Adams

Fairclough

Lyon

et al. 2022

Environ. Sci.: Nano

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Draft Genome Sequences of the Nitrate-Dependent Iron-Oxidizing Proteobacteria Acidovorax sp. Strain BoFeN1 and Paracoccus pantotrophus Strain KS1

Abstract: The draft genomes of the nitrate-dependent iron-oxidizing bacteria Acidovorax sp. strain BoFeN1 and Paracoccus pantotrophus strain KS1 are presented.

Cited by 14 publications

References 16 publications

Impact of reactive surfaces on the abiotic reaction between nitrite and ferrous iron and associated nitrogen and oxygen isotope dynamics

Impact of reactive surfaces on the abiotic reaction between nitrite and ferrous iron and associated nitrogen and oxygen isotope dynamics

Interactions of Vallisneria natans and Iron-Oxidizing Bacteria Enhance Iron-Bound Phosphorus Formation in Eutrophic Lake Sediments

Elucidating heterogeneous iron biomineralization patterns in a denitrifying As(iii)-oxidizing bacterium: implications for arsenic immobilization

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