Draft genome sequences of three multidrug-resistant Cutibacterium (formerly Propionibacterium ) acnes strains isolated from acne patients, China

Zhang, Nanxue; Lü, Zhong; Ma, Ying

doi:10.1016/j.jgar.2017.10.008

Cited by 8 publications

(14 citation statements)

References 7 publications

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“…Therefore, we would have access to all molecular typing levels at the same time, even in the event of horizontal gene transfers, conjugation or recombination. This new age could permit the rapid acquisition of novel types of information, such as virulence genes, and will probably open up new inquiries to investigate resistome and virulome [42]. As soon as this method becomes more commonly used by researchers, it may become the reference method for molecular typing of any bacterium for which sequence types are described and identifiable.…”

Section: Conclusion and Wgs Outlooksmentioning

confidence: 99%

Cutibacterium acnes molecular typing: time to standardize the method

Dagnelie

Khammari

Dréno

et al. 2018

Clinical Microbiology and Infection

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Section: Conclusion and Wgs Outlooksmentioning

confidence: 99%

Cutibacterium acnes molecular typing: time to standardize the method

Dagnelie

Khammari

Dréno

et al. 2018

Clinical Microbiology and Infection

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“…rRNA gene sequences, the molecular basis of fluoroquinolone resistance was not evident. Furthermore, no explanation for the mechanisms driving resistance in the CA17 strain were identified, warranting further study of this strain as previously recommended [21].…”

Section: Impact Statementmentioning

confidence: 89%

“…While all three CA strains displayed moxifloxacin resistance, the underlying mechanisms driving this phenotype have not been identified [21]; moxifloxacin resistance was based on an MIC of 2 mg l −1 [21] which was also previously used in other studies as a resistance breakpoint for this antibiotic in Cutibacterium acnes [52]. Resistance to fluoroquinolones is mostly due to alterations in the target DNA gyrase and topoisomerase IV genes, and amino acid substitutions in gyrA are also the major mechanism responsible for resistance in Cutibacterium acnes [19, 20, 53].…”

Section: Discussionmentioning

confidence: 99%

“…Despite this, the number of whole genome analyses of antibiotic-resistant strains of Cutibacterium acnes have been small and limited to published announcements rather than detailed genetic analyses. In 2017, a genome note by Zhang et al [21] described the whole genome sequence (WGS) results for MDR strains of Cutibacterium acnes (CA17, CA39 and CA51) isolated from acne patients in Shanghai, China. These three strains were found to be resistant to clindamycin (MIC >128 mg l −1 ) erythromycin (MIC >128 mg l −1 ) and moxifloxacin (MIC=2 mg l −1 ).…”

Section: Introductionmentioning

confidence: 99%

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Genetic determinants of antimicrobial resistance in three multi-drug resistant strains of Cutibacterium acnes isolated from patients with acne: a predictive in silico study

Beirne

McCann

McDowell

et al. 2022

Access Microbiology

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Objectives. Using available whole genome data, the objective of this in silico study was to identify genetic mechanisms that could explain the antimicrobial resistance profile of three multi-drug resistant (MDR) strains (CA17, CA51, CA39) of the skin bacterium Cutibacterium acnes previously recovered from patients with acne. In particular, we were interested in detecting novel genetic determinants associated with resistance to fluoroquinolone and macrolide antibiotics that could then be confirmed experimentally. Methods. A range of open source bioinformatics tools were used to ‘mine’ genetic determinants of antimicrobial resistance and plasmid borne contigs, and to characterise the phylogenetic diversity of the MDR strains. Results. As probable mechanisms of resistance to fluoroquinolones, we identified a previously described resistance associated allelic variant of the gyrA gene with a ‘deleterious' S101L mutation in type IA1 strains CA51 (ST1) and CA39 (ST1), as well as a novel E761R ‘deleterious’ mutation in the type II strain CA17 (ST153). A distinct genomic sequence of the efflux protein YfmO which is potentially associated with resistance to MLSB antibiotics was also present in CA17; homologues in CA51, CA39, and other strains of Cutibacterium acnes , were also found but differed in amino acid content. Strikingly, in CA17 we also identified a circular 2.7 kb non-conjugative plasmid (designated pCA17) that closely resembled a 4.8 kb plasmid (pYU39) from the MDR Salmonella enterica strain YU39. Conclusions. This study has provided a detailed explanation of potential genetic determinants for MDR in the Cutibacterium acnes strains CA17, CA39 and CA51. Further laboratory investigations will be required to validate these in silico results, especially in relation to pCA17.

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“…Deptula et al (2018) used the API CHL 50 test strip to assess differences in fermentation profiles of acidipropionibacteria, though without testing for sample identification. Finally, Zhang et al (2017) used the API 20A test system for the identification of C. acnes, although all identified organisms in their study belonged the genus Cutibacterium. The usefulness of API test strips particularly for the identification of cutibacteria is attested in the product brochure of the manufacturer, bioMérieux SA, but their suitability for the identification of dairy PAB is not well documented and questionable.…”

Section: Identification Based On Fermentation Profilesmentioning

confidence: 99%

Propionic acid bacteria in the food industry: An update on essential traits and detection methods

Bücher¹,

Burtscher

Domig

2021

Comp Rev Food Sci Food Safe

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Propionic acid bacteria (PAB) is an umbrella term for a group of bacteria with the ability to produce propionic acid. In the past, due to this common feature and other phenotypic similarities, genetically heterogeneous bacteria were considered as a single genus, Propionibacterium. Members of this genus ranged from "dairy propionibacteria," which are widely known for their role in eye and flavor formation in cheese production, to "cutaneous propionibacteria," which are primarily associated with human skin. In 2016, the introduction of two new genera based on genotypic data facilitated a clear separation of cutaneous (Cutibacterium spp.) from dairy PAB (Propionibacterium spp., Acidipropionibacterium spp.). In light of these taxonomic changes, but with particular emphasis on dairy PAB, this review describes the current state of knowledge about metabolic pathways and other characteristics such as antibiotic resistance and virulence factors. In addition, the relevance of dairy PAB for the food industry and cheese production in particular is highlighted. Furthermore, methods for cultivation, detection, and enumeration are reviewed, incorporating the current taxonomy as well as the potential for routine applications.

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Draft genome sequences of three multidrug-resistant Cutibacterium (formerly Propionibacterium ) acnes strains isolated from acne patients, China

Cited by 8 publications

References 7 publications

Cutibacterium acnes molecular typing: time to standardize the method

Cutibacterium acnes molecular typing: time to standardize the method

Genetic determinants of antimicrobial resistance in three multi-drug resistant strains of Cutibacterium acnes isolated from patients with acne: a predictive in silico study

Propionic acid bacteria in the food industry: An update on essential traits and detection methods

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