Draft genome sequences of two Streptomyces albidoflavus strains DEF1AK and DEF147AK with plant growth-promoting and biocontrol potential

Abstract

Purpose Bacteria belonging to the Streptomyces genus can be exploited in environmentally friendly approaches to food safety. Genome information can help to characterize bioactive strains opening the possibility to decipher their mechanisms of action. Methods The biocontrol and plant growth-promoting activity of two Streptomyces spp. strains, DEF1AK and DEF147AK, were assessed in vitro and in planta. The genome sequences were determined using the Illumina NextSeq sequencing system and were assembled using EvoCAT (Evogene Clustering and Assembly Toolbox). Result Streptomyces spp. DEF1AK and DEF147AK were able to improve seed germination and early plant development of maize, wheat, and tomato and inhibited the mycelium growth of diverse fungal plant pathogens in vitro. The genome sequence analysis identified both strains as S. albidoflavus (99% sequence identity). Both genomes were of 7.1-Mb length with an average GC content of 73.45%. AntiSMASH and MIBiG analyses revealed strain-specific sets of secondary metabolite gene clusters in the two strains as well as differences in the number and type of duplicated genes. Conclusion The combination of the biological activity and genomic data is the basis for in-depth studies aimed at the identification of secondary metabolites involved in plant growth-promoting and biocontrol activity of Streptomyces spp. The comparison of unique genomic features of the two strains will help to explain their diverse biocontrol and plant growth-promoting activities and warrant targeted functional genomics approaches to verify their mechanisms of action.

Streptomyces albidoflavus Strain CARA17 as a Biocontrol Agent against Fungal Soil-Borne Pathogens of Fennel Plants

“…Although a few studies have been carried out to assess the biocontrol efficacy and mechanism of Streptomyces spp. [ 17 , 18 ], to date there has been no research related to the control of soil-borne pathogens on fennel crops.…”

Genome-Guided Investigation Provides New Insights into Secondary Metabolites of Streptomyces parvulus SX6 from Aegiceras corniculatum

“…It is worth noting that huth is duplicated in S. parvulus SX6, which may convert the phenylalanine to yield cinnamic acid. A recent study demonstrated that many duplicated genes, such as ABC transporters, contributed to fitness improvements of Streptomyces albidoflavus DEF1AK in planta conditions (Kunova et al 2021). Thus, this finding supports the well-known assumption that gene duplication acts as a mechanism of genomic adaptation to a changing environment (Bratlie et al 2010).…”

Draft Genome Sequence of Streptomyces sp. Strain R1, Isolated from Water Canal Sediments, Possessing Antimicrobial and Plant Growth Promoting Capabilities

“…Streptomyces are soil-inhabiting, free living, Gram-positive filamentous bacteria that also present as symbionts of plants, insects, and animals ( 1 ). Due to their production of a broad range of bioactive secondary metabolites, Streptomyces have gained attraction in agriculture as biocontrol agents ( 2 – 9 ).…”