Genome-Guided Investigation Provides New Insights into Secondary Metabolites of <i>Streptomyces parvulus</i> SX6 from <i>Aegiceras corniculatum</i>

Quach, Ngoc Tung; Vu, Thi Hanh Nguyen; Bui, Thi Lien; Pham, Anh T.; Nguyen, Thi Thu An; Le, Thi Thanh Xuan; Ta, Thi Thu Thuy; Dudhagara, Pravin; Tien, Phi Quyet

doi:10.33073/pjm-2022-034

Cited by 5 publications

(2 citation statements)

References 52 publications

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“…To evaluate the antimicrobial property of obtained fungi, the agar-well diffusion method was performed with a slight modification (Quach et al, 2022)…”

Section: Antimicrobial Assaymentioning

confidence: 99%

\(\textit{Fusarium fujikuroi}\) WQF5 isolated from \(\textit{Cephalotaxus mannii }\)Hook.f. as a producer of antibacterial agent and paclitaxel

Tung

Phuong

et al. 2022

AJB

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Cephalotaxus mannii Hook.f is a rare medicinal plant used for the treatment of leukemia, however, its fungal endophytes with antibacterial and anticancer properties have not been exploited yet. In this study, six fungal isolates were recovered from roots of C. mannii collected in Ha Giang province, Vietnam. Among them, ethyl acetate crude extract of strain WQF5 exhibited strong antibacterial activity against 5 tested pathogens with inhibition zones ranging from 13.0 ± 0.5 mm to 20.0 ± 0.4 mm. Sulforhodamine B assay showed that WQF5 extract possessed the most potent cytotoxic activity against lung cancer A549 and breast cancer MCF7 cell lines with IC50 values of 6.9 ± 0.7 µg/mL and 23.1 ± 1.9 µg/mL, respectively. PCR-based molecular marker screening revealed that the positive hits for essential genes encoding 10-deacetylbaccatin III-10-O-acetyltransferase (dbat) and C-13 phenylpropanoyl side chain-CoA acyltransferase (bapt) involved in paclitaxel production were found in the fungal isolate WQF5. In addition, isolate WQF5, identified as Fusarium fujikuroi by morphological and ITS analysis, also produced paclitaxel as shown by HPLC-DAD analysis. This is the first report of bioprospecting endophytic fungi isolated from C. mannii, in which the capability of producing paclitaxel of endophytic fungi was also proved for the first time. These findings addressed a potent candidate for paclitaxel production and provided excellent material for further investigations of how endophytic fungi from non-Taxus plant species synthesize paclitaxel.

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“…To evaluate the antimicrobial property of obtained fungi, the agar-well diffusion method was performed with a slight modification (Quach et al, 2022)…”

Section: Antimicrobial Assaymentioning

confidence: 99%

\(\textit{Fusarium fujikuroi}\) WQF5 isolated from \(\textit{Cephalotaxus mannii }\)Hook.f. as a producer of antibacterial agent and paclitaxel

Tung

Phuong

et al. 2022

AJB

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“…Whole-genome sequencing is a costeffective and comprehensive approach to investigate chitinolytic enzymes from bacteria (Quach et al, 2022a;Quach et al, 2022c). The genome of Curtobacterium sp.…”

Section: Introductionmentioning

confidence: 99%

Genome mining reveals chitin degradation potential of \(\textit{Streptomyces parvulus}\) VCCM 22513

Tung

et al. 2023

AJB

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The genus Streptomyces is not only known as a natural producer of antibiotics but also a prolific source of chitinolytic enzymes that digest recalcitrant chitin to chitooligosaccharides. However, only a few reports have used whole-genome sequencing to study chitin degradation of Streptomyces to date. In the present study, out of 22 Streptomyces strains, Streptomyces parvulus VCCM 22513 produced the highest chitinase activity. Time courses of incubation revealed that the maximum chitinase (0.91 ± 0.04 U/mL) of this strain was observed after 96 hours in the yeast extract salts medium supplemented with 10.0 g/L colloidal chitin. Additional genomic analysis of VCCM 22513 was also conducted to discover the genomic information related to chitin degradation. The VCCM 22513 genome consists of 341 CAZy genes divided into 6 families including glycoside hydrolase (134 genes), carbohydrate-binding module (88 genes), glycosyl transferase (87 genes), carbohydrate esterase (18 genes), polysaccharide lyase (7 genes), and auxiliary activity (7 genes). Further genome mining revealed the presence of 10 chitinases, 4 lytic polysaccharide monooxygenases, and 14 β-N-acetylhexosaminidases, which mainly contribute to the degradation of chitin polymers. This is the first report revealing the mechanism underlying the chitin degradation of S. parvulus. Further investigations are required to characterize chitinolytic enzymes found in this study for the bioeconomic production of high-quality chitooligosaccharides from chitin food wastes.

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Metabolic and genomic analysis deciphering biocontrol potential of endophytic Streptomyces albus RC2 against crop pathogenic fungi

Quach,

Vu,

Nguyen

et al. 2023

Braz J Microbiol

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Genome-Guided Investigation Provides New Insights into Secondary Metabolites of Streptomyces parvulus SX6 from Aegiceras corniculatum

Cited by 5 publications

References 52 publications

\(\textit{Fusarium fujikuroi}\) WQF5 isolated from \(\textit{Cephalotaxus mannii }\)Hook.f. as a producer of antibacterial agent and paclitaxel

\(\textit{Fusarium fujikuroi}\) WQF5 isolated from \(\textit{Cephalotaxus mannii }\)Hook.f. as a producer of antibacterial agent and paclitaxel

Genome mining reveals chitin degradation potential of \(\textit{Streptomyces parvulus}\) VCCM 22513

Metabolic and genomic analysis deciphering biocontrol potential of endophytic Streptomyces albus RC2 against crop pathogenic fungi

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