Draft genome sequences of two hypermucoviscous carbapenem-resistant ST25 Klebsiella pneumoniae strains causing respiratory and systemic infections

Jure, María Ángela; Albarracín, Leonardo; Vargas, Juan M. Casa; Maidana, Stefanía Dentice; Zamar, Juan Cortez; Kitazawa, Hideaki; Villena, Julio

doi:10.1016/j.jgar.2021.05.018

Cited by 9 publications

(19 citation statements)

References 5 publications

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“…Our own studies conducted in hospitals in the province of Tucuman (northwestern Argentina) showed that the urinary and respiratory tracts were the most common sources of clinical samples with carbapenem-resistant K. pneumoniae strains, followed by soft tissues and blood [12,13]. In addition, the high incidence of ST25 clones in our region associated with respiratory and systemic infections emphasizes the importance of implementing epidemiological, genetic, and phenotypic studies of this particular ST [14].…”

Section: Introductionmentioning

confidence: 83%

“…An increase in KPC-2-producing hypermucoviscous K. pneumoniae strains belonging to ST25 has been detected in northwestern Argentina in recent years [12,13]. The high incidence of the ST25 clone in our region associated with respiratory and systemic infections emphasizes the importance of implementing more genetic and phenotypic studies of this particular ST [14]. Thus, the first objective of this work was to evaluate the virulence of two strains of K. pneumoniae ST25 isolated from hospitalized patients in a murine model.…”

Section: Discussionmentioning

confidence: 99%

Section: Genomic Characterization Of K Pneumoniae Labacer 01 and Laba...mentioning

confidence: 98%

“…For the experiments, two K. pneumoniae isolates were used: LABACER 01 and LABACER 27. These KPC-2-producing hypermucoviscous K. pneumoniae ST25 strains were selected based on their virulent capacity [14]. The LABACER 01 strain was recovered from a bone sample of a 20-year-old male patient, and the LABACER 27 strain was isolated from a lung sample of a 63-year-old male patient [12].…”

Section: K Pneumoniae Labacer 01 and Labacer 27 Infect The Respirator...mentioning

confidence: 99%

“…We previously reported that the analysis of the K. pneumoniae LABACER 01 genome revealed a size of 5,598,020 bp with 5608 coding sequences and the presence of 19 antimicrobial resistance genes [14]. On the other hand, the LABACER 27 genome analysis showed a size of 5,622,382 bp with 5580 coding sequences and the presence of 20 different antimicrobial resistance genes [14]. In addition, we showed that other general genomic characteristics of the LABACER 01 and 27 strains such as GC% and numbers of tRNAs and rRNAs agreed with those published for other K. pneumoniae strains.…”

Section: Genomic Characterization Of K Pneumoniae Labacer 01 and Laba...mentioning

confidence: 99%

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Genomic and Immunological Characterization of Hypermucoviscous Carbapenem-Resistant Klebsiella pneumoniae ST25 Isolates from Northwest Argentina

Albarracín

Moyano

Vargas

et al. 2022

IJMS

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In recent years, an increase in the prevalence hypermucoviscous carbapenem-resistant Klebsiella pneumoniae with sequence type 25 (ST25) was detected in hospitals of Tucuman (Northwest Argentina). In this work, the virulence and the innate immune response to two K. pneumoniae ST25 strains (LABACER 01 and LABACER 27) were evaluated in a murine model after a respiratory challenge. In addition, comparative genomics was performed with K. pneumoniae LABACER01 and LABACER27 to analyze genes associated with virulence. Both LABACER01 and LABACER27 were detected in the lungs of infected mice two days after the nasal challenge, with LABACER01 counts significantly higher than those of LABACER27. Only LABACER01 was detected in hemocultures. Lactate dehydrogenase (LDH) and albumin levels in bronchoalveolar lavage (BAL) samples were significantly higher in mice challenged with LABACER01 than in LABACER27-infected animals, indicating greater lung tissue damage. Both strains increased the levels of neutrophils, macrophages, TNF-α, IL-1β, IL-6, KC, MCP-1, IFN-γ, and IL-17 in the respiratory tract and blood, with the effect of LABACER01 more marked than that of LABACER27. In contrast, LABACER27 induced higher levels of IL-10 in the respiratory tract than LABACER01. Genomic analysis revealed that K. pneumoniae LABACER01 and LABACER27 possess virulence factors found in other strains that have been shown to be hypervirulent, including genes required for enterobactin (entABCDEF) and salmochelin (iroDE) biosynthesis. In both strains, the genes of toxin–antitoxin systems, as well as regulators of the expression of virulence factors and adhesion genes were also detected. Studies on the genetic potential of multiresistant K. pneumoniae strains as well as their cellular and molecular interactions with the host are of fundamental importance to assess the association of certain virulence factors with the intensity of the inflammatory response. In this sense, this work explored the virulence profile based on genomic and in vivo studies of hypermucoviscous carbapenem-resistant K. pneumoniae ST25 strains, expanding the knowledge of the biology of the emerging ST25 clone in Argentina.

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Section: Introductionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Genomic Characterization Of K Pneumoniae Labacer 01 and Laba...mentioning

confidence: 98%

Section: K Pneumoniae Labacer 01 and Labacer 27 Infect The Respirator...mentioning

confidence: 99%

Section: Genomic Characterization Of K Pneumoniae Labacer 01 and Laba...mentioning

confidence: 99%

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Genomic and Immunological Characterization of Hypermucoviscous Carbapenem-Resistant Klebsiella pneumoniae ST25 Isolates from Northwest Argentina

Albarracín

Moyano

Vargas

et al. 2022

IJMS

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Global spread and evolutionary convergence of multidrug-resistant and hypervirulent Klebsiella pneumoniae high-risk clones

Arcari

Carattoli

2022

Pathogens and Global Health

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Molecular characterization of hypermucoviscous carbapenemase‐encoding Klebsiella pneumoniae isolates from an Egyptian hospital

Ragheb,

Osei Sekyere

2024

Annals of the New York Academy of Sciences

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This study aimed to screen antibiotic resistance and virulence genes in carbapenem‐resistant hypermucoviscous Klebsiella pneumoniae isolates from an Egyptian hospital. Among 38 previously confirmed carbapenem‐nonsusceptible K. pneumoniae isolates, a string test identified three isolates as positive for hypermucoviscosity. Phenotypic characterization and molecular detection of carbapenemase‐ and virulence‐encoding genes were performed. PCR‐based multilocus sequence typing and phylogenetics were used to determine the clonality and global epidemiology of the strains. The coexistence of virulence and resistance genes in the isolates was analyzed statistically using a chi‐square test. Three isolates showed the presence of carbapenemase‐encoding genes (blaNDM, blaVIM, and blaIMP), adhesion genes (fim‐H‐1 and mrkD), and siderophore genes (entB); the isolates belonged to sequence types (STs) 101, 1310, and 1626. The relatedness between these sequence types and the sequence types of globally detected hypermucoviscous K. pneumoniae that also harbor carbapenemases was determined. Our analysis showed that the resistance and virulence profiles were not homogenous. Phylogenetically, different clones clustered together. There was no significant association between the presence of resistance and virulence genes in the isolates. There is a need for periodic surveillance of the healthcare settings in Egypt and globally to understand the true epidemiology of carbapenem‐resistant, hypermucoviscous K. pneumoniae.

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Draft genome sequences of two hypermucoviscous carbapenem-resistant ST25 Klebsiella pneumoniae strains causing respiratory and systemic infections

Cited by 9 publications

References 5 publications

Genomic and Immunological Characterization of Hypermucoviscous Carbapenem-Resistant Klebsiella pneumoniae ST25 Isolates from Northwest Argentina

Genomic and Immunological Characterization of Hypermucoviscous Carbapenem-Resistant Klebsiella pneumoniae ST25 Isolates from Northwest Argentina

Global spread and evolutionary convergence of multidrug-resistant and hypervirulent Klebsiella pneumoniae high-risk clones

Molecular characterization of hypermucoviscous carbapenemase‐encoding Klebsiella pneumoniae isolates from an Egyptian hospital

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