Dramatic changes in blood protein levels during the first week of life in extremely preterm infants

Zhong, Wen; Danielsson, Hanna; Tebani, Abdellah; Karlsson, Max; Elfvin, Anders; Hellgren, Gunnel; Brusselaers, Nele; Brodin, Petter; Hellström, Ann; Fagerberg, Linn; Uhlén, Mathias

doi:10.1038/s41390-020-0912-8

Cited by 17 publications

(29 citation statements)

References 36 publications

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“…The result with high levels of AF in colostrum after both term and preterm birth differs from our previous findings of lower AF levels in placenta after preterm birth (Gustafsson et al, 2018) and may suggest a programming of high levels in colostrum due to the exposure to inflammatory factors for both term and preterm infants immediately after birth. Preterm birth is associated with proteins linked to inflammation (Olin et al, 2018;Zhong et al, 2021). Our results are in line with the findings of a more variable metabolic profile in human milk after preterm birth during the first 4 weeks postpartum (Spevacek et al, 2015).…”

Section: Discussionsupporting

confidence: 90%

Changes in Antisecretory Factor in Human Milk During the Postpartum and Length of Gestation

et al. 2021

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Background Preterm infants are more susceptible to inflammatory complications than term infants. Human milk contains numerous bioactive components protecting the newborn infant. Antisecretory factor, a protein regulating secretory and inflammatory processes by complex binding with complement factors, is present in human milk. Research Aims To describe antisecretory factor (1) in mother’s own milk in term and preterm infants; and (2) in donor milk before and after Holder pasteurization. Methods The study was prospective, longitudinal, explorative, and descriptive. Antisecretory factor-compleasome was determined using sandwich enzyme-linked immunosorbent assay in longitudinal human milk samples over 12 weeks from mothers ( N = 87) of term ( n = 41) and of preterm ( n = 46) infants and 20 anonymized donor human milk samples before and after Holder pasteurization. Results Antisecretory factor-compleasome was overall higher in colostrum versus mature milk ( p < .001) and no difference was found in term or preterm colostrum ( p = .82). In mature milk, compleasome was higher and more variable in the preterm group ( p = .01). After Holder pasteurization, compleasome levels increased ( p < .001). Conclusion Antisecretory factor followed the pattern of other immunological factors with high levels in colostrum. After preterm birth, levels of antisecretory factor were higher and more variable in mature milk. Holder pasteurization did not degrade antisecretory factor, indicating preserved anti-inflammatory properties in donor human milk.

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Section: Discussionsupporting

confidence: 90%

Changes in Antisecretory Factor in Human Milk During the Postpartum and Length of Gestation

et al. 2021

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“…Insights into the longitudinal dynamics of proteins in early preterm life and up to 3 months of life were recently published. [4,27,28] Zhong et al [28] profiled 448 proteins in serum samples from extremely preterm infants followed from birth up to term-equivalent age. Bennike et al, [27] studied the plasma proteome over the first week of life in full-term newborns, highlighting developmental changes in compo-nents of the complement system including C1QB, C4A, and C9 as also seen in our study, suggesting a continued role of the complement at later ages.…”

Section: Discussionmentioning

confidence: 99%

“…Insights into the longitudinal dynamics of proteins in early preterm life and up to 3 months of life were recently published. [ 4,27,28 ] Zhong et al. [ 28 ] profiled 448 proteins in serum samples from extremely preterm infants followed from birth up to term‐equivalent age.…”

Section: Discussionmentioning

confidence: 99%

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Protein profiles in plasma: Development from infancy to 5 years of age

Mikus

Järnbert-Pettersson

Johansson

et al. 2021

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Purpose: Little is known about the longitudinal development of different plasma protein levels during early childhood and particularly in relation to lifestyle factors. This study aimed to monitor the plasma proteome early in life and the influence of different lifestyles. Experimental Design: A multiplex bead-based immunoassay was used to analyze plasma levels of 97 proteins in 280 blood samples longitudinally collected in children at 6, 12, 24, and 60 months of age living in families with an anthroposophic (n = 15), partly anthroposophic (n = 27), or non-anthroposophic (n = 28) lifestyle.Results: A total of 68 proteins (70%) showed significantly altered plasma levels between 6 months and 5 years of age. In lifestyle stratified analysis, 59 of 97 (61%) proteins were altered over time within one or more of the three lifestyle groups. Nearly half of these proteins (28 out of 59) changed irrespective of lifestyle. The temporal changes represented four longitudinal trends of the plasma proteins during development, also following stratification of lifestyle. Conclusions and Clinical Relevance:Our findings contribute to understand the development of the plasma proteome under the influence of lifestyle exposures in early childhood.

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“…Multiplex extension proximity assay technology was used for serum protein analysis (Olink Bioscience, Uppsala, Sweden). 20 , 26 As previously reported in our recent study by Zhong et al, 20 using microliter plates where each well contained 96 pairs of DNA-labeled antibody probes, 92 protein biomarkers could be measured in 88 samples. The data were normalized using both an internal control (extension control) and an inter-plate control and then transformed using a predetermined correction factor to minimize intra- and inter-run variation.…”

Section: Methodsmentioning

confidence: 83%

Blood protein profiles related to preterm birth and retinopathy of prematurity

et al. 2021

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Background Nearly one in ten children is born preterm. The degree of immaturity is a determinant of the infant’s health. Extremely preterm infants have higher morbidity and mortality than term infants. One disease affecting extremely preterm infants is retinopathy of prematurity (ROP), a multifactorial neurovascular disease that can lead to retinal detachment and blindness. The advances in omics technology have opened up possibilities to study protein expressions thoroughly with clinical accuracy, here used to increase the understanding of protein expression in relation to immaturity and ROP. Methods Longitudinal serum protein profiles the first months after birth in 14 extremely preterm infants were integrated with perinatal and ROP data. In total, 448 unique protein targets were analyzed using Proximity Extension Assays. Results We found 20 serum proteins associated with gestational age and/or ROP functioning within mainly angiogenesis, hematopoiesis, bone regulation, immune function, and lipid metabolism. Infants with severe ROP had persistent lower levels of several identified proteins during the first postnatal months. Conclusions The study contributes to the understanding of the relationship between longitudinal serum protein levels and immaturity and abnormal retinal neurovascular development. This is essential for understanding pathophysiological mechanisms and to optimize diagnosis, treatment and prevention for ROP. Impact Longitudinal protein profiles of 14 extremely preterm infants were analyzed using a novel multiplex protein analysis platform combined with perinatal data. Proteins associated with gestational age at birth and the neurovascular disease ROP were identified. Among infants with ROP, longitudinal levels of the identified proteins remained largely unchanged during the first postnatal months. The main functions of the proteins identified were angiogenesis, hematopoiesis, immune function, bone regulation, lipid metabolism, and central nervous system development. The study contributes to the understanding of longitudinal serum protein patterns related to gestational age and their association with abnormal retinal neuro-vascular development.

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Dramatic changes in blood protein levels during the first week of life in extremely preterm infants

Cited by 17 publications

References 36 publications

Changes in Antisecretory Factor in Human Milk During the Postpartum and Length of Gestation

Changes in Antisecretory Factor in Human Milk During the Postpartum and Length of Gestation

Protein profiles in plasma: Development from infancy to 5 years of age

Blood protein profiles related to preterm birth and retinopathy of prematurity

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