Dramatic performance improvement of weak anion-exchange membranes for chromatographic bioseparations

Bhut, Bharat V.; Husson, Scott M.

doi:10.1016/j.memsci.2009.03.046

Cited by 78 publications

(67 citation statements)

References 36 publications

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“…Husson and co-workers (Bhut et al, 2008;Bhut and Husson, 2009) have demonstrated that surface-initiated ATRP can be used to prepare weak anion-exchange adsorptive membranes for bioseparations. For these surface-modified adsorptive membranes, the binding capacity derives exclusively from the polymer nanolayer (Bhut et al, 2008).…”

Section: Resultsmentioning

confidence: 99%

“…The concentration ratio of 2-BIB to 1-BCMEA was varied from 0.25 to 1.0 by keeping the total concentration of 2-BIB þ 1-BCMEA at 18 mM. In a previous study (Bhut and Husson, 2009), we found this concentration to be sufficient to activate the maximum number of -OH groups.…”

Section: Systematic Control Of Polymer Chain Densitymentioning

confidence: 99%

“…Details of the surface-modification process were given in our previous publications (Bhut et al, 2008;Bhut and Husson, 2009). In the first step, RC60 membranes were activated by covalent anchoring of an ATRP initiator.…”

Section: Membrane Surface Modificationmentioning

confidence: 99%

“…In our previous study (Bhut and Husson, 2009), the grafting density was manipulated by using sub-stoichiometric amounts (relative to the number of active -OH groups) of the initiator precursor, 2-BIB, during the membrane-activation step. This method may lead to an uneven distribution of initiator immobilized onto the membrane surface.…”

Section: Preparation Of Strong Anion-exchange Membranesmentioning

confidence: 99%

“…Polymeric tentacles with adsorptive functionality extend into the protein solution that fills the porous volume, providing a scaffold for protein molecules to adsorb. A variety of resin beads for column chromatography (Bowes et al, 2009;Franke et al, 2010;Ghose et al, 2007;Langford et al, 2007;Müller, 1986;Tao and Carta, 2008;Zhang and Sun, 2002) and macroporous membranes for membrane chromatography (Balachandra et al, 2003;Bhut et al, 2008;Bhut and Husson, 2009;He and Ulbricht, 2008;Singh et al, 2008;Tsuneda et al, 1995) have been modified with adsorptive polymeric tentacles. Incorporating polymeric tentacles into macroporous membranes has even greater importance since the surface area per volume of the membrane is much lower than a bed of resin particles (Bhut et al, 2010;Wang et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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The role of polymer nanolayer architecture on the separation performance of anion‐exchange membrane adsorbers: I. Protein separations

Bhut

Weaver

Carter

et al. 2011

Biotech & Bioengineering

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This contribution describes the preparation of strong anion-exchange membranes with higher protein binding capacities than the best commercial resins. Quaternary amine (Q-type) anion-exchange membranes were prepared by grafting polyelectrolyte nanolayers from the surfaces of macroporous membrane supports. A focus of this study was to better understand the role of polymer nanolayer architecture on protein binding. Membranes were prepared with different polymer chain graft densities using a newly developed surface-initiated polymerization protocol designed to provide uniform and variable chain spacing. Bovine serum albumin and immunoglobulin G were used to measure binding capacities of proteins with different size. Dynamic binding capacities of IgG were measured to evaluate the impact of polymer chain density on the accessibility of large size protein to binding sites within the polyelectrolyte nanolayer under flow conditions. The dynamic binding capacity of IgG increased nearly linearly with increasing polymer chain density, which suggests that the spacing between polymer chains is sufficient for IgG to access binding sites all along the grafted polymer chains. Furthermore, the high dynamic binding capacity of IgG (>130 mg/mL) was independent of linear flow velocity, which suggests that the mass transfer of IgG molecules to the binding sites occurs primarily via convection. Overall, this research provides clear evidence that the dynamic binding capacities of large biologics can be higher for well-designed macroporous membrane adsorbers than commercial membrane or resin ion-exchange products. Specifically, using controlled polymerization leads to anion-exchange membrane adsorbers with high binding capacities that are independent of flow rate, enabling high throughput. Results of this work should help to accelerate the broader implementation of membrane adsorbers in bioprocess purification steps.

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Section: Resultsmentioning

confidence: 99%

Section: Systematic Control Of Polymer Chain Densitymentioning

confidence: 99%

Section: Membrane Surface Modificationmentioning

confidence: 99%

Section: Preparation Of Strong Anion-exchange Membranesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The role of polymer nanolayer architecture on the separation performance of anion‐exchange membrane adsorbers: I. Protein separations

Bhut

Weaver

Carter

et al. 2011

Biotech & Bioengineering

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Synthesis Aspects in the Design of Responsive Membranes

Husson

2012

Responsive Membranes and Materials

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Analytical Applications of Membranes

Bruening

2013

Encyclopedia of Membrane Science and Technology

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Roles of membranes in analytical chemistry range from creating potentials in ion‐selective electrodes to catalyzing protein digestion before mass spectrometry. This article focuses on membrane‐based analyte extraction and also briefly discusses recent catalytic membranes that facilitate analyte derivatization or digestion. Membrane‐based liquid‐liquid extraction avoids mixing and subsequent collection of the two phases and the recent development of hollow‐fiber microextraction is particularly attractive for small‐volume, potentially‐automated analyses. Microdialysis sampling is now a widespread tool for analyte collection from a variety of tissues, while membrane introduction mass spectrometry combined with miniaturized mass spectrometers facilitates remote or portable environmental analyses. Derivatization of membranes affords adsorptive materials for solid‐phase extraction. In particular, growth of functionalized polymer brushes in pores yields membrane adsorbers that capture tagged proteins. Moreover, simple methods for enzyme adsorption in porous membranes give reactors that catalyze rapid proteolysis for mass spectrometry. In addition to their history in ion‐selective electrodes and filtration, future membranes will play a significant role in sample purification and derivatization because they provide convenient methods for rapidly concentrating or modifying small‐volume samples.

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Dramatic performance improvement of weak anion-exchange membranes for chromatographic bioseparations

Cited by 78 publications

References 36 publications

The role of polymer nanolayer architecture on the separation performance of anion‐exchange membrane adsorbers: I. Protein separations

The role of polymer nanolayer architecture on the separation performance of anion‐exchange membrane adsorbers: I. Protein separations

Synthesis Aspects in the Design of Responsive Membranes

Analytical Applications of Membranes

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