Draper acts through the JNK pathway to control synchronous engulfment of dying germline cells by follicular epithelial cells

Etchegaray, Jon Iker; Timmons, Allison K.; Klein, Adam P.; Pritchett, Tracy L.; Welch, E. Celeste; Meehan, Tracy L.; Li, Christy; McCall, Kimberly

doi:10.1242/jcs.124966

Cited by 11 publications

(15 citation statements)

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“…Furthermore, these cells upregulate the JNK pathway, as monitored by expression of puc-lacZ (Fig. 7B) similar to the non-professional phagocytic activity of follicle cells in the Drosophila ovary (Etchegaray et al, 2012). Phagocytosis by Sertoli cells in the mammalian testis has been also reported (Chemes, 1986).…”

Section: Sg Death Is Associated With Phagocytosis and Jnk-pathway Actsupporting

confidence: 60%

The regulated elimination of transit-amplifying cells preserves tissue homeostasis during protein starvation in Drosophila testis

Yang

Yamashita

2015

Development

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How tissues adapt to varying nutrient conditions is of fundamental importance for robust tissue homeostasis throughout the life of an organism, but the underlying mechanisms are poorly understood. Here, we show that Drosophila testis responds to protein starvation by eliminating transit-amplifying spermatogonia (SG) while maintaining a reduced pool of actively proliferating germline stem cells (GSCs). During protein starvation, SG die in a manner that is mediated by the apoptosis of somatic cyst cells (CCs) that encapsulate SG and regulate their development. Strikingly, GSCs cannot be maintained during protein starvation when CC-mediated SG death is inhibited, leading to an irreversible collapse of tissue homeostasis. We propose that the regulated elimination of transit-amplifying cells is essential to preserve stem cell function and tissue homeostasis during protein starvation.

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Section: Sg Death Is Associated With Phagocytosis and Jnk-pathway Actsupporting

confidence: 60%

The regulated elimination of transit-amplifying cells preserves tissue homeostasis during protein starvation in Drosophila testis

Yang

Yamashita

2015

Development

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“…[28][29][30][31] Previously, we found that Draper was required earlier in oogenesis for the clearance of nurse cells undergoing apoptosis in response to starvation. 32 Interestingly, we also found that overexpression of Draper in follicle cells was sufficient to kill the underlying germline cells. Thus, our previous work suggested that follicle cells were capable of phagoptosis.…”

Section: Role Of Engulfment Genes In Nurse Cell Deathmentioning

confidence: 65%

Control of non-apoptotic nurse cell death by engulfment genes in Drosophila

Timmons

Mondragon

Meehan

et al. 2016

Fly

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Programmed cell death occurs as a normal part of oocyte development in Drosophila. For each egg that is formed, 15 germline-derived nurse cells transfer their cytoplasmic contents into the oocyte and die. Disruption of apoptosis or autophagy only partially inhibits the death of the nurse cells, indicating that other mechanisms significantly contribute to nurse cell death. Recently, we demonstrated that the surrounding stretch follicle cells non-autonomously promote nurse cell death during late oogenesis and that phagocytosis genes including draper, ced-12, and the JNK pathway are crucial for this process. When phagocytosis genes are inhibited in the follicle cells, events specifically associated with death of the nurse cells are impaired. Death of the nurse cells is not completely blocked in draper mutants, suggesting that other engulfment receptors are involved. Indeed, we found that the integrin subunit, aPS3, is enriched on stretch follicle cells during late oogenesis and is required for elimination of the nurse cells. Moreover, double mutant analysis revealed that integrins act in parallel to draper. Death of nurse cells in the Drosophila ovary is a unique example of programmed cell death that is both non-apoptotic and non-cell autonomously controlled.

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“…When the functions of these genes are removed from winners, the death and engulfment of losers is blocked and the growth advantage of winners is reduced Martín et al, 2009a). In Drosophila and C. elegans, Drpr and its homologue CED-1 are required for the engulfment of apoptotic cells by non-professional phagocytes (Zhou et al, 2001;Etchegaray et al, 2012). Similarly, in competition induced by loss of polarity genes, fragmentation and engulfment of losers by their winner neighbours was observed and captured by live imaging (Ohsawa et al, 2011).…”

Section: Eliminating Cells: the Role Of Engulfmentmentioning

confidence: 99%

Cell competition: how to eliminate your neighbours

2014

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A conventional view of development is that cells cooperate to build an organism. However, based on studies of Drosophila, it has been known for years that viable cells can be eliminated by their neighbours through a process termed cell competition. New studies in mammals have revealed that this process is universal and that many factors and mechanisms are conserved. During cell competition, cells with lower translation rates or those with lower levels of proteins involved in signal transduction, polarity and cellular growth can survive in a homogenous environment but are killed when surrounded by cells of higher fitness. Here, we discuss recent advances in the field as well as the mechanistic steps involved in this phenomenon, which have shed light on how and why cell competition exists in developing and adult organisms.

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Draper acts through the JNK pathway to control synchronous engulfment of dying germline cells by follicular epithelial cells

Cited by 11 publications

References 1 publication

The regulated elimination of transit-amplifying cells preserves tissue homeostasis during protein starvation in Drosophila testis

The regulated elimination of transit-amplifying cells preserves tissue homeostasis during protein starvation in Drosophila testis

Control of non-apoptotic nurse cell death by engulfment genes in Drosophila

Cell competition: how to eliminate your neighbours

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