Dressings Loaded with Cyclodextrin–Hamamelitannin Complexes Increase <i>Staphylococcus aureus</i> Susceptibility Toward Antibiotics Both in Single as well as in Mixed Biofilm Communities

Brackman, Gilles; Garcia-Fernandez, Maria José; Lenoir, Joke; Meyer, Laurens De; Remon, Jean‐Paul; Beer, Thomas De; Concheiro, Angel; Álvarez-Lorenzo, Carmen; Coenye, Tom

doi:10.1002/mabi.201500437

Cited by 64 publications

(50 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Artificial dermis composed of hyaluronic acid and collagen was used in our wound model, as described before (Brackman et al, 2016). Each disk of artificial dermis was placed in 24-well microtiter plate.…”

Section: Methodsmentioning

confidence: 99%

Pitfalls associated with evaluating enzymatic quorum quenching activity: the case of MomL and its effect onPseudomonas aeruginosaandAcinetobacter baumanniibiofilms

Zhang

Brackman

Coenye

2017

PeerJ

Self Cite

View full text Add to dashboard Cite

Background. The enzymatic degradation of quorums sensing (QS) molecules (called quorum quenching, QQ) has been considered as a promising anti-virulence therapy to treat biofilm-related infections and combat antibiotic resistance. The recentlydiscovered QQ enzyme MomL has been reported to efficiently degrade different N -acyl homoserine lactones (AHLs) of various Gram-negative pathogens. Here we investigated the effect of MomL on biofilms formed by two important nosocomial pathogens, Pseudomonas aeruginosa and Acinetobacter baumannii. Methods. MomL was expressed in E.coli BL21 and purified. The activity of MomL on AHLs with hydroxyl substituent was tested. Biofilms of P. aeruginosa PAO1 and Acinetobacter strains were formed in 96-well microtiter plates. Biofilm formation was evaluated by crystal violet staining, plating and fluorescence microscopy. The effect of MomL on biofilm susceptibility to antibiotics was also tested. We further evaluated MomL in dual-species biofilms formed by P. aeruginosa and A. baumannii, and in biofilms formed in a wound model. The effect of MomL on virulence of A. baumannii was also tested in the Caenorhabditis elegans model. Results. MomL reduced biofilm formation and increased biofilm susceptibility to different antibiotics in biofilms of P. aeruginosa PAO1 and A. baumannii LMG 10531 formed in microtiter plates in vitro. However, no significant differences were detected in the dual-species biofilm and in wound model biofilms. In addition, MomL did not affect virulence of A. baumannii in the C. elegans model. Finally, the effect of MomL on biofilm of Acinetobacter strains seems to be strain-dependent. Discussion. Our results indicate that although MomL showed a promising anti-biofilm effect against P. aeruginosa and A. baumanii biofilms formed in microtiter plates, the effect on biofilm formation under conditions more likely to mimic the real-life situation was much less pronounced or even absent. Our data indicate that in order to obtain a better picture of potential applicability of QQ enzymes for the treatment of biofilmrelated infections, more elaborate model systems need to be used.

show abstract

Section: Methodsmentioning

confidence: 99%

Pitfalls associated with evaluating enzymatic quorum quenching activity: the case of MomL and its effect onPseudomonas aeruginosaandAcinetobacter baumanniibiofilms

Zhang

Brackman

Coenye

2017

PeerJ

Self Cite

View full text Add to dashboard Cite

show abstract

“…For example, HAM and a more potent derivative showed high potentiating activity in a mouse model for S. aureus mastitis [31,32]. By using an in vitro wound biofilm model, we could demonstrate that it is feasible to load wound care dressings with cyclodextrin-HAM complexes to increase the effect of antibiotics [99], opening the road towards further pharmaceutical developments. Finally, an exciting option is combining several of the novel approaches outlined above.…”

Section: Discussionmentioning

confidence: 98%

Innovative approaches to treat Staphylococcus aureus biofilm-related infections

Richter

Driessche

Coenye

2017

Essays in Biochemistry

Self Cite

View full text Add to dashboard Cite

Many bacterial infections in humans and animals are caused by bacteria residing in biofilms, complex communities of attached organisms embedded in an extracellular matrix. One of the key properties of microorganisms residing in a biofilm is decreased susceptibility towards antimicrobial agents. This decreased susceptibility, together with conventional mechanisms leading to antimicrobial resistance, makes biofilm-related infections increasingly difficult to treat and alternative antibiofilm strategies are urgently required. In this review, we present three such strategies to combat biofilm-related infections with the important human pathogen : (i) targeting the bacterial communication system with quorum sensing (QS) inhibitors, (ii) a 'Trojan Horse' strategy to disturb iron metabolism by using gallium-based therapeutics and (iii) the use of 'non-antibiotics' with antibiofilm activity identified through screening of repurposing libraries.

show abstract

“…21 Recently, Brackman and colleagues developed HP--CD functionalized cellulose gauzes loaded with the antibiotic vancomycin or/ and QS inhibitor hamamelitannin (HAM). 22 The HP--CD functionalized gauze with vancomycin was capable of inhibiting bio¯lm formation of S. aureus with or without the presence of Pseudomonas aeruginosa, and its bio¯lm inhibitory e®ect was even greater when loaded with both vancomycin and HAM.…”

Section: Cyclodextrins Loaded With Antibioticsmentioning

confidence: 95%

Cyclodextrins: A Weapon in the Fight Against Antimicrobial Resistance

Wong

Dolzhenko

Lee

et al. 2017

J. Mol. Eng. Mater.

View full text Add to dashboard Cite

Antimicrobial resistance poses one of the most serious global challenges of our age. Cyclodextrins (CDs) are widely utilized excipients in formulations because of their solubilizing properties, low toxicity, and low in°ammatory response. This review summarizes recent investigations of antimicrobial agents involving CDs and CD-based antimicrobial materials. CDs have been employed for antimicrobial applications either through formation of inclusion complexes or by chemical modi¯cation of their hydroxyl groups to tailor pharmaceutically active compounds. Applications of these CD inclusion complexes include drug delivery, antimicrobial coatings on materials (e.g., biomedical devices and implants) and antimicrobial dressings that help to prevent wound infections. There are relatively limited studies of chemically modi¯ed CDs with antimicrobial activity. The mechanism of action of antimicrobial CD inclusion complexes and derivatives needs further elucidation, but activity of CDs and their derivatives is often associated with their interaction with bacterial cell membranes.

show abstract

Dressings Loaded with Cyclodextrin–Hamamelitannin Complexes Increase Staphylococcus aureus Susceptibility Toward Antibiotics Both in Single as well as in Mixed Biofilm Communities

Cited by 64 publications

References 51 publications

Pitfalls associated with evaluating enzymatic quorum quenching activity: the case of MomL and its effect onPseudomonas aeruginosaandAcinetobacter baumanniibiofilms

Pitfalls associated with evaluating enzymatic quorum quenching activity: the case of MomL and its effect onPseudomonas aeruginosaandAcinetobacter baumanniibiofilms

Innovative approaches to treat Staphylococcus aureus biofilm-related infections

Cyclodextrins: A Weapon in the Fight Against Antimicrobial Resistance

Contact Info

Product

Resources

About