Dried blood spot analysis of an iron chelator – Deferasirox and its potential application to therapeutic drug monitoring

Nirogi, Ramakrishna; Ajjala, Devender Reddy; Kandikere, Vishwottam; Aleti, Raghupathi; Srikakolapu, SuryaRao; Himabindu, V.

doi:10.1016/j.jchromb.2012.08.036

Cited by 15 publications

(7 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Over the last decade, a rising number of DBS assays for different classes of drugs have been reported. So far, antidiabetics, immunosuppressants, analgesics, anti-HIV drugs, antihypertensive drugs, antimicrobial agents, anti-epileptics, and the iron chelator deferasirox have been included [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25]. Among the antidepressants, a DBS assay for analysis of IMP has previously been described as well as for the SSRIs fluoxetine, reboxetine, and paroxetine, and for the atypical antidepressant venlafaxine [26][27][28].…”

Section: Introductionmentioning

confidence: 99%

A simple dried blood spot method for therapeutic drug monitoring of the tricyclic antidepressants amitriptyline, nortriptyline, imipramine, clomipramine, and their active metabolites using LC-MS/MS

Berm

Paardekooper

Brummel-Mulder

et al. 2015

Talanta

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

A simple dried blood spot method for therapeutic drug monitoring of the tricyclic antidepressants amitriptyline, nortriptyline, imipramine, clomipramine, and their active metabolites using LC-MS/MS

Berm

Paardekooper

Brummel-Mulder

et al. 2015

Talanta

View full text Add to dashboard Cite

“…Several advantages of the DBS include low blood volume requirement, transportation and storage without special treatment, better analyte stability, enhanced clinical cooperation and reduced unforeseeable exposure of analysts to biohazards; these make it the most appropriate blood sampling technique. Recently a numerous publications have reported on DBS (Mohamed et al ., ; Ramesh et al ., ; Kole et al ., ; Nageswara Rao et al ., ; Nirogi et al ., ). The present manuscript describes the development and validation of a rat DBS analysis method to evaluate individually the pharmacokinetics of (+) and (−) DRV enantiomers on immobilized tris‐(3, 5‐dimethylphenylcarbasmate) amylase by LC.…”

Section: Introductionmentioning

confidence: 97%

Dried blood spot analysis of (+) and (−) darunavir enantiomers on immobilized amylose tris‐(3, 5‐dimethylphenylcarbamate) LC and its application to pharmacokinetics

Ramisetti

Arnipalli

Nimmu

2015

Biomedical Chromatography

View full text Add to dashboard Cite

Dried blood spot analysis is an innovative novel blood sampling technique gaining interest in drug discovery and development processes owing to its inherent advantages over the conventional whole blood, plasma or serum sample collection. The present manuscript describes the development and validation of a highly sensitive and precise method of evaluation of pharmacokinetics of (+) and (-) darunavir enantiomers on rat dried blood spots. The enantiomers on rat dried blood spots were extracted into methanol and separated by LC on a Chiralpak IA column using hexane and ethanol containing 0.1% DEA (75:25, v/v) as a mobile phase at 20°C; both the enantiomers were detected at 266 nm using a photodiode array detector. The method was validated in terms of selectivity, linearity, accuracy, precision and stability as per the US Food and Drug and Administration guidelines. The hematocrit effect on extraction recovery was evaluated and the mean recoveries of (-) and (+) enantiomers of darunavir from dried blood spots were found to be 85.76 and 88.91% respectively. The intra- and inter-day precision and accuracy were 3.1-8.4 and 0.8-4.8% respectively. The developed method was successfully applied to a pharmacokinetic study of (+) and (-) enantiomers of darunavir on rat dried blood spots.

show abstract

“…Due to these adverse effects, it is very important to establish an easy-operating method for monitoring the DFX concentration in a patient's plasma. Until now, a lot of analytical methods have been developed for determining DFX in plasma, and most of them have utilized HPLC coupled with UV, fluorescence, or MS/MS [33][34][35][36][37]. Recently, a stacking CE technique [38] was proposed for measuring DFX in biological fluid.…”

Section: Introductionmentioning

confidence: 99%

Stable Luminescent Poly(Allylaminehydrochloride)-Templated Copper Nanoclusters for Selectively Turn-Off Sensing of Deferasirox in β-Thalassemia Plasma

et al. 2021

View full text Add to dashboard Cite

Highly stable and facile one-pot copper nanoclusters (Cu NCs) coated with poly(allylamine hydrochloride) (PAH) have been synthesized for selectively sensing deferasirox (DFX) in β-thalassemia plasma. DFX is an important drug used for treating iron overloading in β-thalassemia, but needs to be monitored due to certain toxicity. In this study, the PAH-Cu NCs showed highly stable fluorescence with emission wavelengths at 450 nm. The DFX specifically interacted with the copper nanocluster to turn off the fluorescence of the PAH-Cu NCs, and could be selectively quantified through the fluorescence quenching effect. The linear range of DFX in plasma analyzed by PAH-Cu NCs was 1.0–100.0 µg/mL (r = 0.985). The relative standard deviation (RSD) and relative error (RE) were lower than 6.51% and 7.57%, respectively, showing excellent reproducibility of PAH-Cu NCs for sensing DFX in plasma. This method was also successfully applied for an analysis of three clinical plasma samples from β-thalassemia patients taking DFX. The data presented high similarity with that obtained through a capillary electrophoresis method. According to the results, the PAH-Cu NCs could be used as a tool for clinically sensing DFX in human plasma for clinical surveys.

show abstract

Dried blood spot analysis of an iron chelator – Deferasirox and its potential application to therapeutic drug monitoring

Cited by 15 publications

References 29 publications

A simple dried blood spot method for therapeutic drug monitoring of the tricyclic antidepressants amitriptyline, nortriptyline, imipramine, clomipramine, and their active metabolites using LC-MS/MS

A simple dried blood spot method for therapeutic drug monitoring of the tricyclic antidepressants amitriptyline, nortriptyline, imipramine, clomipramine, and their active metabolites using LC-MS/MS

Dried blood spot analysis of (+) and (−) darunavir enantiomers on immobilized amylose tris‐(3, 5‐dimethylphenylcarbamate) LC and its application to pharmacokinetics

Stable Luminescent Poly(Allylaminehydrochloride)-Templated Copper Nanoclusters for Selectively Turn-Off Sensing of Deferasirox in β-Thalassemia Plasma

Contact Info

Product

Resources

About