Dried Urine Swabs as a Tool for Monitoring Metabolite Excretion

Monošík, Rastislav; Dragsted, Lars Ove

doi:10.4155/bio-2018-0042

Cited by 11 publications

(5 citation statements)

References 27 publications

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“…Dried urine has also been used to measure other urine analytes of interest, such as sodium and potassium, with similarly high levels of stability 62 . As with others who used either lter paper or cotton swabs 63 , we found that dried urine results are in agreement with liquid urine results, reduced the burden on patients, and had good stability over time 12 . Still, agreement between 24-h urine collections and early-morning, single spot urine collections for hormonal analysis are often poor 64 .…”

Section: Discussionsupporting

confidence: 88%

Reliability of A Dried Urine Test for Comprehensive Assessment of Urine Hormones and Metabolites

Newman¹,

Curran²

2020

Preprint

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BackgroundMass spectrometry allows for analysis of multiple hormone and organic acid metabolites from small urine volumes; however, to assess the full extent of daily hormone production, 24-hour urine collections are usually required. The aims of this study were, first, to confirm that mass spectrometric analysis of an array of hormones and organic acids would yield similar results in both liquid and dried urine, and, second, to determine if collection of four dried spot urine samples could be substituted for a 24-hour collection when measuring reproductive hormones. MethodsTwo study populations were included in this prospective observational study. Twenty individuals collected both a spot liquid urine and dried urine on filter paper to analyze eight organic acids. A second group of 26 individuals collected both a 24-hour urine and four dried spot urines during waking hours throughout the same day for evaluation of 17 reproductive hormones and metabolites; data from 18 of these individuals were available to compare liquid versus dried urine results. Dried urine was extracted, hydrolyzed, and derivatized before analysis by mass spectrometry; all analytes from dried urine were normalized to urine creatinine. ResultsReproductive hormone results from dried and liquid urine were in excellent agreement with intraclass correlation coefficients (ICCs) greater than 0.90; comparison of dried to liquid urine for organic acids showed good to excellent agreement (ICC range: 0.75 to 0.99). Comparison between the 4-spot urine collection and 24-hour urine collection methods showed excellent agreement (ICC>0.9) for 14 of the 17 urine metabolites and good agreement for the others (ICC 0.78 to 0.85) with no systematic differences between the two methods of collection. ConclusionsThe burden of urine collection can be reduced using collection of four spot dried urines on filter paper without compromising comparability with hormone results from a 24-hour urine collection. A large number of urine analytes can be assessed from the dried urine with similar results to those from liquid urine. Given the ease of sample handling, this 4-spot dried urine assay would be useful for both clinical assessment of patients and for large epidemiologic studies.

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Section: Discussionsupporting

confidence: 88%

Reliability of A Dried Urine Test for Comprehensive Assessment of Urine Hormones and Metabolites

Newman¹,

Curran²

2020

Preprint

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“…The MetAl trial aimed to identify new alcohol biomarkers in blood, urine, and hair [ 83 ] and was without a wash-out period due to the aim of exploring acute changes in intake biomarkers with changes in alcohol intake. Therefore, the study is prone to carry-over effects, and carry-over was observed for one variable, HDL 2a -apoA1.…”

Section: Discussionmentioning

confidence: 99%

1–2 Drinks Per Day Affect Lipoprotein Composition after 3 Weeks—Results from a Cross-Over Pilot Intervention Trial in Healthy Adults Using Nuclear Magnetic Resonance-Measured Lipoproteins and Apolipoproteins

et al. 2022

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Alcohol consumption ranging from 1–2 drinks/day associates with a lower risk of coronary heart disease in some studies. The underlying mechanisms are unclear. The Metabolic Imprints of Alcoholic Beverages (MetAl) trial aimed to explore the short-term effects of moderate alcohol consumption on cardiovascular biomarkers. A 2 × 3-week cross-over single-blinded intervention trial investigating the effect of 1–2 drinks/day (~12–24 g) compared with abstention on 1H Nuclear Magnetic Resonance-measured main lipoproteins and subfractions was performed in 26 healthy adults. Volunteers were classified as occasional or habitual drinkers based on their habitual alcohol intakes (<2 or ≥2 drinks/week). Compared with abstention, 1–2 drinks/day increased HDL2a-C (p = 0.004), HDL3-C (p = 0.008), and HDL non-significantly (p = 0.19). Total apoA1 and apoA1 in HDL and its subfractions increased (p < 0.05). Novel findings were a decreased apoB/apoA1 ratio (p = 0.02), and increased HDL2a phospholipid content (p = 0.04). In women alone, the results were similar but attenuated, and LDL-P decreased. Thus, changes in apoA1- and HDL-related biomarkers occur within weeks in moderate drinkers. Compared with abstention, 1–2 drinks/day increased total apoA1 more strongly than HDL-C and increased the cholesterol, apoA1, and phospholipid content of several HDL subfractions. Whether this provides a cardiovascular benefit requires further study. Clinicaltrials.gov: NCT03384147.

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“…The first is a white paper coming out of a recent Japan Bioanalysis Forum meeting, detailing outcomes of the discussions concerning the best selection strategy for surrogate matrices as applied to both LC-MS and ligand binding assays [1]. Following this, there is a fascinating comparison of data, obtained by Monošík and Dragsted, using dried urine swab samples against wet urine samples for the quantitative determination by UHPLC-MS of a suitably chemically diverse range of dietary metabolites [2]. Subsequently there is a report of the successful LC-MS/MS method validation by Ferreira et al, using a water-based surrogate matrix, of dexamethasone in human aqueous humor [3].…”

Section: Recent Thinking and Empirical Outcomesmentioning

confidence: 99%

Analytical Convergence in Surrogate Control Matrices

MacNeill

2018

Bioanalysis

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Dried Urine Swabs as a Tool for Monitoring Metabolite Excretion

Abstract: Although this technique seems very promising more methodological studies have to be conducted in order to improve applicability of dried urine microvolume fluidic sampling for quantitative analysis.

Cited by 11 publications

References 27 publications

Reliability of A Dried Urine Test for Comprehensive Assessment of Urine Hormones and Metabolites

Reliability of A Dried Urine Test for Comprehensive Assessment of Urine Hormones and Metabolites

1–2 Drinks Per Day Affect Lipoprotein Composition after 3 Weeks—Results from a Cross-Over Pilot Intervention Trial in Healthy Adults Using Nuclear Magnetic Resonance-Measured Lipoproteins and Apolipoproteins

Analytical Convergence in Surrogate Control Matrices

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