Food waste is a hot topic around the world due to the significant environmental challenge it poses. The study aims to assess the impact of food waste on the food chain at the national level. The data were obtained from quantitative impact studies, carried out in a project funded by the Ministry of Agriculture and Sustainable Development, “Methods to reduce food waste on the agri-food chain, at national level, to prevent and reduce socio-economic impact, until 2030”. A total of 852 companies were interviewed, with a turnover of almost 6.5 billion euro and a number of over 69 thousand employees, including 273 primary production enterprises, 270 food processing units, 171 distribution/retail units, and 138 HoReCa units.