Droplet-based microfluidic synthesis of nanogels for controlled drug delivery: tailoring nanomaterial properties <i>via</i> pneumatically actuated flow-focusing junction

Giannitelli, Sara Maria; Limiti, Emanuele; Mozetic, Pamela; Pinelli, Filippo; Han, Xiaoyu; Abbruzzese, Franca; Basoli, Francesco; Rio, Danila Del; Scialla, Stefano; Rossi, Filippo; Trombetta, Marcella; Rosanò, Laura; Gigli, Giuseppe; Zhang, Zhenyu J.; Mauri, Emanuele; Rainer, Alberto

doi:10.1039/d2nr00827k

Cited by 11 publications

(3 citation statements)

References 77 publications

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“…4C enabled the synthesis of polymeric NPs via droplet-based microfluidics. 72 The polymers hyaluronic acid (HA) and linear polyethyleneimine (LEPI) were covalently crosslinked in each droplet to produce highly monodisperse polymeric NPs with tunable sizes of 92-190 nm and a very small PDI of 0.015. The release of encapsulated DOX exhibited an enhanced antiblastic effect even at sublethal dosages, highlighting the applicability of this droplet-based microfluidic method in nanomedicine scenarios.…”

Section: Polymeric Npsmentioning

confidence: 99%

Advanced manufacturing of nanoparticle formulations of drugs and biologics using microfluidics

Shen,

Gwak,

Han

2024

Analyst

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Section: Polymeric Npsmentioning

confidence: 99%

Advanced manufacturing of nanoparticle formulations of drugs and biologics using microfluidics

Shen,

Gwak,

Han

2024

Analyst

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“…This approach offers on-demand and controlled drug delivery by controlling the magnetic field strength, exposure time, and amount of loaded nanoparticles. One study employed a droplet-based microfluidic device to synthesize polymeric nanocarriers [ 152 ]. Nanogels were generated using a flow-focusing microfluidic device.…”

Section: Droplet-based Microfluidic Technology For Pharmaceutical App...mentioning

confidence: 99%

Droplet-Based Microfluidics: Applications in Pharmaceuticals

Trinh,

Do,

Nam

et al. 2023

Pharmaceuticals

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Droplet-based microfluidics offer great opportunities for applications in various fields, such as diagnostics, food sciences, and drug discovery. A droplet provides an isolated environment for performing a single reaction within a microscale-volume sample, allowing for a fast reaction with a high sensitivity, high throughput, and low risk of cross-contamination. Owing to several remarkable features, droplet-based microfluidic techniques have been intensively studied. In this review, we discuss the impact of droplet microfluidics, particularly focusing on drug screening and development. In addition, we surveyed various methods of device fabrication and droplet generation/manipulation. We further highlight some promising studies covering drug synthesis and delivery that were updated within the last 5 years. This review provides researchers with a quick guide that includes the most up-to-date and relevant information on the latest scientific findings on the development of droplet-based microfluidics in the pharmaceutical field.

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“…In material engineering, microdroplets offer an effective method for the synthesis of uniform materials and execution of high-throughput material screening, attributable to the precise control of reaction conditions within each droplet [11,12]. Microdroplets further facilitate the development of novel polymers and colloids, pertinent to emerging applications such as drug delivery technologies [13,14].…”

Section: Introductionmentioning

confidence: 99%

An Injection-Mold Based Method with a Nested Device for Microdroplet Generation by Centrifugation

Li,

et al. 2024

Processes

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Microdroplets have been widely used in different fields due to their unique properties, such as compartmentalization, single-molecule sensitivity, chemical and biological compatibility, and high throughput. Compared to intricate and labor-intensive microfluidic techniques, the centrifuge-based method is more convenient and cost-effective for generating droplets. In this study, we developed a handy injection molding based method to readily produce monodisperse droplets by centrifugation. Briefly, we used two three-dimensional (3D) printed master molds with internal cavities to forge two coupled sub-molds by injecting polydimethylsiloxane (PDMS) and casted these two PDMS sub-molds into a nested structure that clamps the micro-channel array (MiCA) by injecting polyurethane resin. This method enables the generation of various sizes of monodispersed microdroplets by centrifugation with proper parameters within 10 min. To assess the performance of this method, homogeneous fluorescent hydrogel microspheres were generated and droplet digital polymerase chain reaction (ddPCR) was carried out. Overall, this method offers high-throughput droplet generation, reduces costs compared to other methods, and is user-friendly.

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Droplet-based microfluidic synthesis of nanogels for controlled drug delivery: tailoring nanomaterial properties via pneumatically actuated flow-focusing junction

Abstract: A droplet-based microfluidic process relying on an actuated flow-focusing device was used for the in-flow synthesis of hyaluronic acid-polyethyleneimine nanogels with improved drug delivery properties.

Cited by 11 publications

References 77 publications

Advanced manufacturing of nanoparticle formulations of drugs and biologics using microfluidics

Advanced manufacturing of nanoparticle formulations of drugs and biologics using microfluidics

Droplet-Based Microfluidics: Applications in Pharmaceuticals

An Injection-Mold Based Method with a Nested Device for Microdroplet Generation by Centrifugation

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