Droplet-based Synthesis of Homogeneous Gold Nanoparticles for Enhancing HRP-based ELISA Signals

Choi, Ji Wook; Kim, Young Jae; Lee, Jong Min; Choi, Jin‐Ha; Choi, Jeong‐Woo; Chung, Bong Geun

doi:10.1007/s13206-020-4307-z

Cited by 25 publications

(18 citation statements)

References 44 publications

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“…In this method, antibodies are immobilized on a solid support, and the target analytes in the samples are specifically bound to the immobilized antibodies. The amount of the bound target analyte is quantified by the treatment with enzyme-linked antibodies, and the enzyme reaction with the treated substrate causes quantifiable signals based on color, fluorescence, or chemiluminescence. ,,,,,− …”

Section: Resultsmentioning

confidence: 99%

Cesium Lead Bromide (CsPbBr₃) Perovskite Quantum Dot-Based Photosensor for Chemiluminescence Immunoassays

Kim

Bong

Park

et al. 2021

ACS Appl. Mater. Interfaces

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Chemiluminescence immunoassays have been widely employed for diagnosing various diseases. However, because of the extremely low intensity chemiluminescence signals, highly sensitive transducers, such as photomultiplier tubes and image sensors with cooling devices, are required to overcome this drawback. In this study, a hypersensitive photosensor was developed based on cesium lead bromide (CsPbBr 3 ) perovskite quantum dots (QDs) with sufficient high sensitivity for chemiluminescence immunoassays. First, CsPbBr 3 QDs with a highly uniform size, that is, 5 nm, were synthesized under thermodynamic control to achieve a high size confinement effect. For the fabrication of the photosensor, MoS 2 nanoflakes were used as an electron transfer layer and heat-treated at an optimum temperature. Additionally, a parylene-C film was used as a passivation layer to improve the physical stability and sensitivity of the photosensor. In particular, the trap states on the CsPbBr 3 QDs were reduced by the passivation layer, and the sensitivity was increased. Finally, a photosensor based on CsPbBr 3 QDs was employed in chemiluminescence immunoassays for the detection of human hepatitis B surface antigen, human immunodeficiency virus antibody, and alpha-fetoprotein (AFP, a cancer biomarker). When compared with the conventionally used equipment, the photosensor was determined to be feasible for application in chemiluminescence immunoassays.

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Section: Resultsmentioning

confidence: 99%

Cesium Lead Bromide (CsPbBr₃) Perovskite Quantum Dot-Based Photosensor for Chemiluminescence Immunoassays

Kim

Bong

Park

et al. 2021

ACS Appl. Mater. Interfaces

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“…These nanoparticles were utilized as per nanocarriers signaling antirespiratory syncytial virus‐HRP for amplification of signal. So, the production of uniform nanoparticles in controlled manner could play a significant part in enhancing signals for sensing that depends on gold nanoparticles [119]. Dense nanowires of ZnO having variable lengths and diameters are attained via altering polyethyleneimine content besides the time for growth in hydrothermal method occurring inside microchannels for recognition of proteins.…”

Section: Applications Of Microfluidic‐based Nanoparticlesmentioning

confidence: 99%

Microfluidic‐based nanoparticle synthesis and their potential applications

et al. 2021

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Microfluidic-based nanoparticle synthesis and their potential applicationsA lot of substantial innovation in advancement of microfluidic field in recent years to produce nanoparticle reveals a number of distinctive characteristics, for instance, compactness, controllability, fineness in process, and stability along with minimal reaction amount. Recently, a prompt development, as well as realization in the production of nanoparticles in microfluidic environment having dimension of micro to nanometers and constituents extending from metals, semiconductors to polymers, has been made. Microfluidics technology integrates fluid mechanics for the production of nanoparticles having exclusive with homogenous sizes, shapes, and morphology, which are utilized in several bioapplications such as biosciences, drug delivery, and healthcare including food engineering. Nanoparticles are usually well-known for having fine and rough morphology because of their small dimensions including exceptional physical, biological, chemical, and optical properties. Though the orthodox procedures need huge instruments, costly autoclaves, use extra power, extraordinary heat loss, as well as take surplus time for synthesis. Additionally, this is fascinating to systematize, assimilate, in addition, to reduce traditional tools onto one platform to produce micro and nanoparticles. The synthesis of nanoparticles by microfluidics permits fast handling besides better efficacy of method utilizing the smallest components for process. Herein, we will focus on synthesis of nanoparticles by means of microfluidic devices intended for different bioapplications.

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“…Furthermore, surface modification of the gold surface can be easily achieved using a thiol group via the gold–thiol covalent attachment. These properties provide a convenient functionalization approach for AuNPs, particularly for their application in biomedical fields (Choi et al, 2020 ). In the electrochemical biosensing field, the fine electrical conductivity of AuNPs is useful when the target biomolecules are present in a small amount and the electrochemical signals from the redox reaction of biosensor samples are extremely small.…”

Section: Noble Metal Nanomaterials For Biosensorsmentioning

confidence: 99%

Noble Metal Nanomaterial-Based Biosensors for Electrochemical and Optical Detection of Viruses Causing Respiratory Illnesses

Choi

Lee

Lee³

et al. 2021

Front. Chem.

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Noble metal nanomaterials, such as gold, silver, and platinum, have been studied extensively in broad scientific fields because of their unique properties, including superior conductivity, plasmonic property, and biocompatibility. Due to their unique properties, researchers have used them to fabricate biosensors. Recently, biosensors for detecting respiratory illness-inducing viruses have gained attention after the global outbreak of coronavirus disease (COVID-19). In this mini-review, we discuss noble metal nanomaterials and associated biosensors for detecting respiratory illness-causing viruses, including SARS-CoV-2, using electrochemical and optical detection techniques. this review will provide interdisciplinary knowledge about the application of noble metal nanomaterials to the biomedical field.

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Droplet-based Synthesis of Homogeneous Gold Nanoparticles for Enhancing HRP-based ELISA Signals

Cited by 25 publications

References 44 publications

Cesium Lead Bromide (CsPbBr₃) Perovskite Quantum Dot-Based Photosensor for Chemiluminescence Immunoassays

Cesium Lead Bromide (CsPbBr₃) Perovskite Quantum Dot-Based Photosensor for Chemiluminescence Immunoassays

Microfluidic‐based nanoparticle synthesis and their potential applications

Noble Metal Nanomaterial-Based Biosensors for Electrochemical and Optical Detection of Viruses Causing Respiratory Illnesses

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Droplet-based Synthesis of Homogeneous Gold Nanoparticles for Enhancing HRP-based ELISA Signals

Cited by 25 publications

References 44 publications

Cesium Lead Bromide (CsPbBr3) Perovskite Quantum Dot-Based Photosensor for Chemiluminescence Immunoassays

Cesium Lead Bromide (CsPbBr3) Perovskite Quantum Dot-Based Photosensor for Chemiluminescence Immunoassays

Microfluidic‐based nanoparticle synthesis and their potential applications

Noble Metal Nanomaterial-Based Biosensors for Electrochemical and Optical Detection of Viruses Causing Respiratory Illnesses

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Product

Resources

About

Cesium Lead Bromide (CsPbBr₃) Perovskite Quantum Dot-Based Photosensor for Chemiluminescence Immunoassays

Cesium Lead Bromide (CsPbBr₃) Perovskite Quantum Dot-Based Photosensor for Chemiluminescence Immunoassays