Droplet Mixer based on Siphon-Induced Flow Discretization and Phase Shifting

Burger, Robert; Reis, Nuno; Fonseca, João F. B. D.; Ducrée, Jens

doi:10.1109/memsys.2009.4805414

Cited by 5 publications

(5 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5i). 83 To circumvent the demand for hydrophilic coatings, siphon priming by the release of pneumatic energy (eqn (6)) from an enclosed and compressed air bubble was exploited in the so-called ''pneumatic siphon valve'' (Fig. 5j).…”

Section: Valving and Switchingmentioning

confidence: 99%

“…The protein concentrations in all of the aliquots showed good agreement with the value expected for a perfect mixture. 83 Liebeskind et al used the catalytic decomposition of H 2 O 2 to water and oxygen as an on-chip gas source to generate gas bubbles for mixing. The generated gas was pumped into a mixing chamber, where, due to the buoyancy force in the artificial gravitational field, the bubbles moved through the liquids to be mixed and caused perturbations.…”

Section: Mixingmentioning

confidence: 99%

“…The protein concentrations in all of the aliquots showed good agreement with the value expected for a perfect mixture. 83…”

Section: Unit Operationsmentioning

confidence: 99%

See 2 more Smart Citations

Centrifugal microfluidic platforms: advanced unit operations and applications

et al. 2015

View full text Add to dashboard Cite

Centrifugal microfluidics has evolved into a mature technology. Several major diagnostic companies either have products on the market or are currently evaluating centrifugal microfluidics for product development. The fields of application are widespread and include clinical chemistry, immunodiagnostics and protein analysis, cell handling, molecular diagnostics, as well as food, water, and soil analysis. Nevertheless, new fluidic functions and applications that expand the possibilities of centrifugal microfluidics are being introduced at a high pace. In this review, we first present an up-to-date comprehensive overview of centrifugal microfluidic unit operations. Then, we introduce the term "process chain" to review how these unit operations can be combined for the automation of laboratory workflows. Such aggregation of basic functionalities enables efficient fluidic design at a higher level of integration. Furthermore, we analyze how novel, ground-breaking unit operations may foster the integration of more complex applications. Among these are the storage of pneumatic energy to realize complex switching sequences or to pump liquids radially inward, as well as the complete pre-storage and release of reagents. In this context, centrifugal microfluidics provides major advantages over other microfluidic actuation principles: the pulse-free inertial liquid propulsion provided by centrifugal microfluidics allows for closed fluidic systems that are free of any interfaces to external pumps. Processed volumes are easily scalable from nanoliters to milliliters. Volume forces can be adjusted by rotation and thus, even for very small volumes, surface forces may easily be overcome in the centrifugal gravity field which enables the efficient separation of nanoliter volumes from channels, chambers or sensor matrixes as well as the removal of any disturbing bubbles. In summary, centrifugal microfluidics takes advantage of a comprehensive set of fluidic unit operations such as liquid transport, metering, mixing and valving. The available unit operations cover the entire range of automated liquid handling requirements and enable efficient miniaturization, parallelization, and integration of assays.

show abstract

Section: Valving and Switchingmentioning

confidence: 99%

Section: Mixingmentioning

confidence: 99%

See 1 more Smart Citation

Centrifugal microfluidic platforms: advanced unit operations and applications

et al. 2015

View full text Add to dashboard Cite

show abstract

“…This design allowed the fluids to be subsequently distributed to a designated area through multiple acceleration and deceleration operations [ 42 ]. Burger et al presented an overflow siphon valve where liquid filled the chamber and was held back by the outlet siphon until the crest point of the siphon was reached [ 43 ]. Godino et al presented a centrifugo-pneumatic siphon system.…”

Section: Advanced Unit Operationsmentioning

confidence: 99%

A Review of Biomedical Centrifugal Microfluidic Platforms

et al. 2016

View full text Add to dashboard Cite

Centrifugal microfluidic or lab-on-a-disc platforms have many advantages over other microfluidic systems. These advantages include a minimal amount of instrumentation, the efficient removal of any disturbing bubbles or residual volumes, and inherently available density-based sample transportation and separation. Centrifugal microfluidic devices applied to biomedical analysis and point-of-care diagnostics have been extensively promoted recently. This paper presents an up-to-date overview of these devices. The development of biomedical centrifugal microfluidic platforms essentially covers two categories: (i) unit operations that perform specific functionalities, and (ii) systems that aim to address certain biomedical applications. With the aim to provide a comprehensive representation of current development in this field, this review summarizes progress in both categories. The advanced unit operations implemented for biological processing include mixing, valving, switching, metering and sequential loading. Depending on the type of sample to be used in the system, biomedical applications are classified into four groups: nucleic acid analysis, blood analysis, immunoassays, and other biomedical applications. Our overview of advanced unit operations also includes the basic concepts and mechanisms involved in centrifugal microfluidics, while on the other hand an outline on reported applications clarifies how an assembly of unit operations enables efficient implementation of various types of complex assays. Lastly, challenges and potential for future development of biomedical centrifugal microfluidic devices are discussed.

show abstract

“…These capillary siphons thus stay closed at high frequencies while priming below a certain threshold. In an overflow siphon, liquid is simply added to the upstream reservoir until the siphon overflows [10]. Siphoning is therefore triggered by an external event, the addition of a volume to the reservoir.…”

Section: Introductionmentioning

confidence: 99%

Plasma extraction by centrifugo-pneumatically induced gating of flow

Burger

Reis²,

Fonseca³

et al. 2013

J. Micromech. Microeng.

View full text Add to dashboard Cite

We present a novel valving mechanism to implement plasma extraction from whole blood on a centrifugal microfluidic ‘lab-on-a-disc’ platform. The new scheme is based on pressure-induced deflection of a liquid membrane which gates the centrifugally driven flow through a microfluidic structure. Compared to conventional concepts such as capillary burst valves, siphons or sacrificial materials, the valving structure presented here is represented by a compact, small-footprint design which obviates the need for (local) surface functionalization or hybrid materials integration, thus significantly reducing the complexity (and hence cost) of manufacture. As a pilot study of this new centrifugal flow control element, we demonstrate here the efficient separation of metered plasma from whole blood. While the flow is stopped, blood is separated into plasma and its cellular constituents by centrifugally induced sedimentation. After completion, the flow resumes by elevating the spinning frequency, providing up to 80% of the original plasma content to an overflow chamber within a short, 2 min interval. The amount of residual cells in the plasma amounts to less than 20 cells μl−1.

show abstract

Droplet Mixer based on Siphon-Induced Flow Discretization and Phase Shifting

Cited by 5 publications

References 6 publications

Centrifugal microfluidic platforms: advanced unit operations and applications

Centrifugal microfluidic platforms: advanced unit operations and applications

A Review of Biomedical Centrifugal Microfluidic Platforms

Plasma extraction by centrifugo-pneumatically induced gating of flow

Contact Info

Product

Resources

About