DropletMicroarray: facile formation of arrays of microdroplets and hydrogel micropads for cell screening applications

Ueda, Erica; Geyer, Florian; Nedashkivska, Victoria; Levkin, Pavel A.

doi:10.1039/c2lc40921f

Cited by 167 publications

(156 citation statements)

References 32 publications

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“…The size and volume of the droplets depend on the geometry and surface area of the hydrophilic regions and is controllable from 700 pL up to 3 µL. 18 This allows for the dispensing of aqueous solutions into thousands of droplets without the need for manual pipetting or robotic devices. As soon as the nanoporous SL areas become wet, the porous polymer becomes transparent due to reduced light scattering caused by matched refractive indexes, thereby making SL spots visible and enabling light and fluorescent microscopy analysis.…”

Section: Resultsmentioning

confidence: 99%

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Fabrication of Hydrogel Particles of Defined Shapes Using Superhydrophobic‐Hydrophilic Micropatterns

et al. 2016

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We report a method to rapidly fabricate alginate hydrogel particles of specific sizes and shapes.Our method is based on the formation of arrays of droplets of pre-hydrogel solutions on superhydrophobic-hydrophilic patterns using the process of discontinuous dewetting, followed by their gelation via the parallel addition of CaCl 2 to the individual droplets via the sandwiching method. We demonstrate that viability of living cells incorporated within the hydrogel particles is higher during the long-term cultivation than in the case of cells cultured in the bulk threedimensional hydrogel matrix. Incorporation of magnetic particles into the free-standing hydrogel particles containing living cells enabled ease manipulation of the particles using an external magnetic field.

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Section: Resultsmentioning

confidence: 99%

“…[18][19][20][21][22] The extreme wettability contrast of the SH-SL patterns allows the spontaneous separation of an aqueous solution into high-density arrays of microdroplets using the effect of discontinuous dewetting. This rapid and facile droplet formation does not require multiple pipetting or a liquid handling device.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Hydrogel Particles of Defined Shapes Using Superhydrophobic‐Hydrophilic Micropatterns

et al. 2016

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“…We demonstrated the formation of droplet microarray using the effect of discontinuous dewetting 38 , the formation of arrays of hydrogel micropads 38 and DMA Sandwiching Chip for the parallel addition of chemical libraries to the individual droplets 37 . In this work we demonstrated how to solve the problems of the live cell microarrays developed by Sabatini using the inherent compartmentalization capability of the DMA platform.…”

Section: Introductionmentioning

confidence: 99%

Droplet-microarray on superhydrophobic–superhydrophilic patterns for high-throughput live cell screenings

et al. 2016

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Cell-based high content phenotypic screenings are widely used in fundamental research, pharmaceutical industry, and healthcare to simultaneously evaluate the effects of multiple compounds or gene over-expressions/knockdown on the phenotype of cells. Most screenings, especially in the industrial sector, are performed using microplate technology, which relies on high reagents and cell consumption as well as on expensive liquid handling robotics. Developing miniaturized screening platforms has been an important topic in the past decade. In this study we demonstrate the applicability of the Droplet-Microarray platform based on superhydrophobic-superhydrophilic patterning for cell-based high throughput screenings. We show the homogeneous seeding of cells and culturing of different adherent cell lines in individual droplets of different sizes. We demonstrate pipetting-free medium exchange enabling cell cultures in miniaturized droplet arrays for up to 5 days. We establish the method of reverse transfection and reverse drug screenings in individual nanoliter-size droplets by printing the transfection mixtures or drug molecules directly onto superhydrophilic spots prior to cell seeding.

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“…Low surface-energy beyond the boundary of the high surface-energy patterns induced the separation of small droplets. 16 Furthermore, superhydrophilic and superhydrophobic patterns remained intact under bending impact owing to the exibility of hybrid ormosil that preserves its structure under mechanical stress (Fig. 4d).…”

Section: Resultsmentioning

confidence: 97%

“…13,14 Therefore, patterned superhydrophobic surfaces have attracted signicant research interest for high-throughput biological and chemical screening. [15][16][17][18] Over the past decade, a number of successful methods have been introduced to prepare superhydrophilicsuperhydrophobic micropatterns including photo-induced chemical modication, [18][19][20][21][22] plasma treatment, 4,[23][24][25] inkprinting, 6,26-28 direct laser writing 29 and photo-catalytic decomposition. 5,[30][31][32][33][34][35] Alternatively, UV/O treatment offers a very simple and cost-effective way for superhydrophilic patterning.…”

Section: Introductionmentioning

confidence: 99%

Robust superhydrophilic patterning of superhydrophobic ormosil surfaces for high-throughput on-chip screening applications

Beyazkılıç¹,

Tuvshindorj²,

Yıldırım³

et al. 2016

RSC Adv.

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This article describes a facile method for the preparation of two-dimensionally patterned superhydrophobic hybrid coatings with controlled wettability. Superhydrophobic coatings were deposited from nanostructured organically modified silica (ormosil) colloids that were synthesized via a simple sol-gel method. On the defined areas of the superhydrophobic ormosil coatings, stable wetted micropatterns were produced using Ultraviolet/Ozone (UV/O) treatment which modifies the surface chemistry from hydrophobic to hydrophilic without changing the surface morphology. The degree of wettability can be precisely controlled depending on the UV/O exposure duration; extremely wetted spots with water contact angle (WCA) of nearly 0 can be obtained. Furthermore, we demonstrated high-throughput biomolecular adsorption and mixing using the superhydrophilic patterns. The proposed superhydrophilic-patterned nanostructured ormosil surfaces with their simple preparation, robust and controlled wettability as well as adaptability on flexible substrates, hold great potential for biomedical and chemical on-chip analysis.

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DropletMicroarray: facile formation of arrays of microdroplets and hydrogel micropads for cell screening applications

Cited by 167 publications

References 32 publications

Fabrication of Hydrogel Particles of Defined Shapes Using Superhydrophobic‐Hydrophilic Micropatterns

Fabrication of Hydrogel Particles of Defined Shapes Using Superhydrophobic‐Hydrophilic Micropatterns

Droplet-microarray on superhydrophobic–superhydrophilic patterns for high-throughput live cell screenings

Robust superhydrophilic patterning of superhydrophobic ormosil surfaces for high-throughput on-chip screening applications

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