Dropping in on the lipid droplet- tumor protein D52 (TPD52) as a new regulator and resident protein

Chen, Yuyan; Frost, Sarah; Byrne, Jennifer A.

doi:10.1080/21623945.2016.1148835

Cited by 9 publications

(9 citation statements)

References 46 publications

(100 reference statements)

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“…Two isoforms of Tpd52 like protein 2b were also elevated at 25°C. Tpd52 associates with lipid droplets and likely signifies changes in lipid storage (70).…”

Section: Discussionmentioning

confidence: 99%

Chronic temperature stress effects on the liver proteome of two threespine stickleback (Gasterosteus aculeatus) populations using a novel DIA assay library

Levitan

Kültz

2021

Preprint

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A data-independent acquisition (DIA) assay library was generated for the liver of threespine sticklebacks to evaluate alterations in protein abundance and functional enrichment of molecular pathways following either chronic warm (25°C) or cold (7°C) three-week temperature challenge in two estuarine populations. The DIA assay library was created from a data-dependent acquisition (DDA) based raw spectral library that was filtered to remove low quality or ambiguous peptides. Functional enrichment analyses using STRING identified larger networks that were significantly enriched by examining both the entire liver proteome and only significantly elevated or depleted proteins from the various comparisons. These systems level analyses revealed the unique liver proteomic signatures of two populations of threespine sticklebacks acclimated to chronic temperature stress. The Big lagoon population (BL) had a stronger response than the Klamath river population (KL). At 7°C, BL showed alterations in protein homeostasis that likely fueled a higher demand for energy, but both populations successfully acclimated to this temperature. The warm acclimation induced major increases in proteins involved in chromatin structure and transcription, while there were decreases in proteins related to translation and fatty acid metabolism. Functional enrichment analyses of the entire liver proteome uncovered differences in glycolysis and carbohydrate metabolism between the two populations and between the cold acclimated and control groups. We conclude that the synchronous regulatory patterns of many proteins observed in the liver of threespine sticklebacks provide more comprehensive insight into population-specific responses to thermal stress than the use of less specific pre-determined biomarkers.

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“…Two isoforms of Tpd52 like protein 2b were also elevated at 25°C. Tpd52 associates with lipid droplets and likely signifies changes in lipid storage (70).…”

Section: Discussionmentioning

confidence: 99%

Chronic temperature stress effects on the liver proteome of two threespine stickleback (Gasterosteus aculeatus) populations using a novel DIA assay library

Levitan

Kültz

2021

Preprint

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“…Its genetic sequence is located on chromosome 8 (8q21). It is actually a peripheral membrane and cytosolic protein of the mammalian gene family that performs several biological functions including vesicle trafficking and lipid metabolism [ 23 ]. Its aberrant expression in several cancers such as prostate, ovarian, and colorectal cancer, adenocarcinoma, hepatocellular carcinoma, and renal and adrenocortical carcinoma had been widely reported [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Unravelling Structure, Localization, and Genetic Crosstalk of KLF3 in Human Breast Cancer

Khan

Safi

Abbas

et al. 2020

BioMed Research International

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Breast cancer is the most prevailing disease among women. It actually develops from breast tissue and has heterogeneous and complex nature that constitutes multiple tumor quiddities. These features are associated with different histological forms, distinctive biological characteristics, and clinical patterns. The predisposition of breast cancer has been attributed to a number of genetic factors, associated with the worst outcomes. Unfortunately, their behavior with relevance to clinical significance remained poorly understood. So, there is a need to further explore the nature of the disease at the transcriptome level. The focus of this study was to explore the influence of Krüppel-like factor 3 (KLF3), tumor protein D52 (TPD52), microRNA 124 (miR-124), and protein kinase C epsilon (PKCε) expression on breast cancer. Moreover, this study was also aimed at predicting the tertiary structure of KLF3 protein. Expression of genes was analyzed through real-time PCR using the delta cycle threshold method, and statistical significance was calculated by two-way ANOVA in Graphpad Prism. For the construction of a 3D model, various bioinformatics software programs, Swiss Model and UCSF Chimera, were employed. The expression of KLF3, miR-124, and PKCε genes was decreased (fold change: 0.076443, 0.06969, and 0.011597, respectively). However, there was 2-fold increased expression of TPD52 with p value < 0.001 relative to control. Tertiary structure of KLF3 exhibited 80.72% structure conservation with its template KLF4 and was 95.06% structurally favored by a Ramachandran plot. These genes might be predictors of stage, metastasis, receptor, and treatment status and used as new biomarkers for breast cancer diagnosis. However, extensive investigations at the tissue level and in in vivo are required to further strengthen their role as a potential biomarker for prognosis of breast cancer.

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“…In 2016, researchers demonstrated that the exogenous expression of human tumor protein D52 (TPD52) in the cultured 3T3 cells result in a significant increase in the numbers of lipid droplets [7]. Starting with the bulging of a triglyceride lens within the endoplasmic reticulum bilayer, lipid droplet biogenesis factors including TPD52 are recruited to the lens structure and facilitate the growth of the nascent lipid droplet [8,9].…”

mentioning

confidence: 99%

“…Tumor protein D52 is the founding member of the TPD52-like protein family representing four paralogous mammalian genes, i.e. TPD52, TPD52L1, TPD52L2, and TPD52L3 [7,13,14]. The group of Cao demonstrated that human TPD52L3 interacted with itself and with TPD52, TPD52L1, and TPD52L2 [14].…”

mentioning

confidence: 99%

Identification of The Downregulation of TPD52-Like3 Gene and NKX2-1 Gene in Type 2 Diabetes Mellitus Via RNA Sequencing

Weijers

2020

ADO

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In an earlier study, it was proposed that the final consequence of hereditary anomaly results in the development of type 2 diabetes, which already emerges in the prediabetic phase. It was thought to occur due to an increased flux, as compared to the healthy controls where protons (H + -ions) from the mitochondrial intermembranespace re-enter the matrix via uncoupling protein-1 (UCP1). This causes hyperthermia in and around the mitochondria [1].But the key question that remains to be answered here is for the connection between the increased flow of protons and type 2 diabetes mellitus. In the past decade, a study reported on the visual documentation of the possible interaction of lipid droplets with mitochondria. This interaction was found to be quite intimate with the involvement of membrane attached receptor proteins such as SNAP23 [2]. Also, the cellular population of mitochondria in brown adipocytes tissue could be divided into two subpopulations; i.e. mitochondrial population having physical evidence of adherence to a lipid droplet or peridroplet mitochondria, and a non-lipid droplet-bound cytoplasmic mitochondrial population without any adherence to lipid droplets [3,4].Although both the peridroplet mitochondria and cytoplasmic mitochondria are similar in their cell membrane composition they differ in other fundamental respects [3,4]. A comparison of the purified peridroplet mitochondria to cytoplasmic mitochondria suggests that peridroplet mitochondria are more elongated, whereas cytoplasmic mitochondria tend to be smaller. Also, peridroplet mitochondria have enhanced oxidative phosphorylation capacity, TCA cycle activity, ATP synthesis, as well as increased ATP-dependent triglyceride synthesis compared to cytoplasmic mitochondria. The measured fatty acid-driven respiration and UCP1 content in the isolated mitochondria suggests that for thermogenic fat oxidation peridroplet mitochondria are not specialized compared to cytoplasmic mitochondria [3]. This signifies that, under healthy conditions, in the peridroplet mitochondria the protons derived from free fatty acids (FFAs) and generated by the electron-transport chain during the oxidation process of FA are used for the production of ATP without any escape of protons via UCP1 to produce heat. On the other hand, the protons generated by the oxidation of cytoplasmic mitochondrial FA are mainly used for the production of heat. So, peridroplet mitochondria have an

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Dropping in on the lipid droplet- tumor protein D52 (TPD52) as a new regulator and resident protein

Cited by 9 publications

References 46 publications

Chronic temperature stress effects on the liver proteome of two threespine stickleback (Gasterosteus aculeatus) populations using a novel DIA assay library

Chronic temperature stress effects on the liver proteome of two threespine stickleback (Gasterosteus aculeatus) populations using a novel DIA assay library

Unravelling Structure, Localization, and Genetic Crosstalk of KLF3 in Human Breast Cancer

Identification of The Downregulation of TPD52-Like3 Gene and NKX2-1 Gene in Type 2 Diabetes Mellitus Via RNA Sequencing

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