Drosophila architectural protein CTCF is not essential for fly survival and is able to function independently of CP190

Kyrchanova, Olga; Klimenko, Natalia; Postika, Nikolay; Bonchuk, Artem; Zolotarev, Nikolay; Maksimenko, Oksana; Georgiev, Pavel

doi:10.1016/j.bbagrm.2021.194733

Cited by 17 publications

(35 citation statements)

References 92 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Transgenic lines were constructed to express dCTCF and Pita mutants incapable of interacting with CP190. CP190 binding to CTCF- and Pita-dependent promoters was not distorted, indicating that other, still unknown C2H2 architectural protein recruited CP190 ( Kyrchanova et al, 2021 ; Sabirov et al, 2021 ).…”

Section: The Role Of Cooperation Between Architectural Proteins In Th...mentioning

confidence: 97%

“…However, in Drosophila remains unstudied how the inactivation of the cohesin complex affects the formation of TAD and long-range interactions between regulatory elements. Drosophila CTCF (dCTCF) lost some of its functions, and ctcf null mutants are consequently viable ( Kyrchanova et al, 2021 ). dCTCF has fewer than 1,000 binding sites that co-localize with only a minor part of TAD boundaries ( Fudenberg and Nora, 2021 ; Kaushal et al, 2021 ).…”

Section: The Role Of Cohesin In Chromosome Organization In Mammals An...mentioning

confidence: 99%

“…The Drosophila C2H2 architectural proteins also work cooperatively to form active promoters. A large group of architectural C2H2 proteins, including M1BP, CTCF, Su(Hw), and Pita, ensures the promoter localization of the TF CP190 ( Bag et al, 2021 ; Kyrchanova et al, 2021 ; Sabirov et al, 2021 ). CP190 is important for recruiting the transcription complexes to promoters ( Maksimenko et al, 2020 ).…”

Section: The Role Of Cooperation Between Architectural Proteins In Th...mentioning

confidence: 99%

See 2 more Smart Citations

Mechanisms of enhancer-promoter communication and chromosomal architecture in mammals and Drosophila

2022

View full text Add to dashboard Cite

The spatial organization of chromosomes is involved in regulating the majority of intranuclear processes in higher eukaryotes, including gene expression. Drosophila was used as a model to discover many transcription factors whose homologs play a key role in regulation of gene expression in mammals. According to modern views, a cohesin complex mostly determines the architecture of mammalian chromosomes by forming chromatin loops on anchors created by the CTCF DNA-binding architectural protein. The role of the cohesin complex in chromosome architecture is poorly understood in Drosophila, and CTCF is merely one of many Drosophila architectural proteins with a proven potential to organize specific long-range interactions between regulatory elements in the genome. The review compares the mechanisms responsible for long-range interactions and chromosome architecture between mammals and Drosophila.

show abstract

Section: The Role Of Cooperation Between Architectural Proteins In Th...mentioning

confidence: 97%

Section: The Role Of Cohesin In Chromosome Organization In Mammals An...mentioning

confidence: 99%

Section: The Role Of Cooperation Between Architectural Proteins In Th...mentioning

confidence: 99%

See 1 more Smart Citation

Mechanisms of enhancer-promoter communication and chromosomal architecture in mammals and Drosophila

2022

View full text Add to dashboard Cite

show abstract

“…Although solved with better resolution compared to the other known structures of the CP190 BTB domain (PDB codes 4U77 and 5EUP), this new structure demonstrates only minor differences (see Supplementary Materials Data for a detailed description). Previously, we mapped the 13-aa region (220-232 aa) in Pita and the 19-aa region (715-733 aa) in dCTCF that are critical for interaction with the BTB domain [45,48]. Pita also has a second minor region (114-164 aa) that interacts with the BTB domain with low affinity [45] (Figure 4a).…”

Section: Identification Of Key Amino Acids In Btb Involved In Interactions With C2h2 Proteinsmentioning

confidence: 99%

“…In addition to the BTB domain, the D and M domains in CP190 (Figure 2a) were also shown to interact with the architectural proteins. The dCTCF protein interacts simultaneously with the BTB and M domains of CP190 [48], while the ZIPIC protein interacts only with the M domain [35]. The Opbp protein interacts with the aspartic acid-rich D-region (D domain, 245-309 aa) of CP190 [36].…”

Section: The Cg31365 and Cg4730 Proteins Interact Not Only With The Btb But Also With The M And D Domains Of Cp190mentioning

confidence: 99%

Mechanisms of CP190 Interaction with Architectural Proteins in Drosophila Melanogaster

Sabirov

Popovich

Boyko

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

Most of the known Drosophila architectural proteins interact with an important cofactor, CP190, that contains three domains (BTB, M, and D) that are involved in protein–protein interactions. The highly conserved N-terminal CP190 BTB domain forms a stable homodimer that interacts with unstructured regions in the three best-characterized architectural proteins: dCTCF, Su(Hw), and Pita. Here, we identified two new CP190 partners, CG4730 and CG31365, that interact with the BTB domain. The CP190 BTB resembles the previously characterized human BCL6 BTB domain, which uses its hydrophobic groove to specifically associate with unstructured regions of several transcriptional repressors. Using GST pull-down and yeast two-hybrid assays, we demonstrated that mutations in the hydrophobic groove strongly affect the affinity of CP190 BTB for the architectural proteins. In the yeast two-hybrid assay, we found that architectural proteins use various mechanisms to improve the efficiency of interaction with CP190. Pita and Su(Hw) have two unstructured regions that appear to simultaneously interact with hydrophobic grooves in the BTB dimer. In dCTCF and CG31365, two adjacent regions interact simultaneously with the hydrophobic groove of the BTB and the M domain of CP190. Finally, CG4730 interacts with the BTB, M, and D domains of CP190 simultaneously. These results suggest that architectural proteins use different mechanisms to increase the efficiency of interaction with CP190.

show abstract

Mechanoresponsive regulation of myogenesis by the force-sensing transcriptional regulator Tono

Zhang,

Avellaneda,

Spletter

et al. 2024

Current Biology

View full text Add to dashboard Cite

Drosophila architectural protein CTCF is not essential for fly survival and is able to function independently of CP190

Cited by 17 publications

References 92 publications

Mechanisms of enhancer-promoter communication and chromosomal architecture in mammals and Drosophila

Mechanisms of enhancer-promoter communication and chromosomal architecture in mammals and Drosophila

Mechanisms of CP190 Interaction with Architectural Proteins in Drosophila Melanogaster

Mechanoresponsive regulation of myogenesis by the force-sensing transcriptional regulator Tono

Contact Info

Product

Resources

About