Drosophila cuticular proteins with the R&amp;R Consensus: Annotation and classification with a new tool for discriminating RR-1 and RR-2 sequences

Karouzou, Maria V.; Spyropoulos, Yannis; Iconomidou, Vassiliki A.; Cornman, Robert S.; Hamodrakas, Stavros J.; Willis, Judith H.

doi:10.1016/j.ibmb.2007.03.007

Cited by 150 publications

(148 citation statements)

References 22 publications

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“…Proteomics analyses by MS/MS of various cuticle preparations identified peptides, either unique or shared among similar proteins, for 93% of the 156 CPR proteins, validating their authenticity as cuticular proteins (Cornman et al, 2007;He et al, 2007). The present study was designed to answer the following questions that might help us to understand why An.…”

Section: Introductionmentioning

confidence: 94%

“…Proteins with the R&R Consensus have been classified as belonging to the CPR protein family. This family has two distinct groups, RR-1 and RR-2 (Andersen, 1998;Karouzou et al, 2007). CPRs *Corresponding author: Judith H. Willis,with the RR-1 type domain have been attributed to soft (flexible) cuticles, while RR-2 proteins have been associated with rigid (hard) cuticle, although this classification is tentative (Andersen, 2000;Willis et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…CPRs *Corresponding author: Judith H. Willis,with the RR-1 type domain have been attributed to soft (flexible) cuticles, while RR-2 proteins have been associated with rigid (hard) cuticle, although this classification is tentative (Andersen, 2000;Willis et al, 2005). A third group, RR-3, was also proposed (Andersen, 2000), but a precise definition has not been established (Karouzou et al, 2007). Predictions that the R&R Consensus serves to bind to chitin have been supported in various ways.…”

Section: Introductionmentioning

confidence: 99%

“…We have annotated 156 CPR genes from the whole genome sequence of Anopheles gambiae (Cornman et al, 2007). The CPR genes are found as singletons and in tandem arrays of linked genes.…”

Section: Introductionmentioning

confidence: 99%

“…Genes coding for RR-1 and RR-2 proteins do not co-exist in the same tandem array. Within tandem arrays there are also sequence clusters of genes that code for very similar RR-2 proteins (Cornman et al, 2007). Members of a sequence cluster are generally sequentially arranged on a chromosome, but a few were found at a distance from the main cluster with other less closely related RR-2 genes intervening.…”

Section: Introductionmentioning

confidence: 99%

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Developmental expression patterns of cuticular protein genes with the R&R Consensus from Anopheles gambiae

Togawa

Dunn

Emmons

et al. 2008

Insect Biochemistry and Molecular Biology

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106

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CPR proteins are the largest cuticular protein family in arthropods. The whole genome sequence of Anopheles gambiae revealed 156 genes that code for proteins with the R&R Consensus and named CPRs. This protein family can be divided into RR-1 and RR-2 subgroups, postulated to contribute to different regions of the cuticle. We determined the temporal expression patterns of these genes throughout post-embryonic development by means of real-time qRT-PCR. Based on expression profiles, these genes were grouped into 21 clusters. Most of the genes were expressed with sharp peaks at single or multiple periods associated with molting. Genes coding for RR-1 and RR-2 proteins were found together in several co-expression clusters. Twenty-five genes were expressed exclusively at one metamorphic stage. Five out of six X-linked genes showed equal expression in males and females, supporting the presence of a gene dosage compensation system in An. gambiae. Many RR-2 genes are organized into sequence clusters whose members are extremely similar to each other and generally closely associated on a chromosome. Most genes in each sequence cluster are expressed with the same temporal expression pattern and at the same level, suggesting a shared mechanism to regulate their expression.

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Section: Introductionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…We have annotated 156 CPR genes from the whole genome sequence of Anopheles gambiae (Cornman et al, 2007). The CPR genes are found as singletons and in tandem arrays of linked genes.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Developmental expression patterns of cuticular protein genes with the R&R Consensus from Anopheles gambiae

Togawa

Dunn

Emmons

et al. 2008

Insect Biochemistry and Molecular Biology

Self Cite

106

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Biopolymers from Animals

Shaik

Moussian

2013

Handbook of Biopolymer‐Based Materials

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CLONING, EXPRESSION PATTERNS, AND PRELIMINARY CHARACTERIZATION OF AccCPR24, A NOVEL RR‐1 TYPE CUTICLE PROTEIN GENE FROM Apis cerana cerana

Chu

Zhang

et al. 2013

Arch Insect Biochem Physiol

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Cuticular proteins (CPs) are key components of insect cuticle, a structure that plays a pivotal role in insect development and defense. In this study, we cloned the full-length cDNA of a CP gene from Apis cerana cerana (AccCPR24). An amino acid sequence alignment indicated that AccCPR24 contains the conserved Rebers and Riddiford consensus sequence and shares high similarity with the genes from other hymenopteran insects. We then isolated the genomic DNA and found that the first intron, which is present in other CP genes, is absent in AccCPR24. Real-time quantitative polymerase chain reaction (qPCR) analysis revealed that AccCPR24 is highly expressed in the late pupal stage and midgut. Expression was inhibited by an exogenous ecdysteroid in vitro but was enhanced by this hormone in vivo; environmental stressors, such as heavy metals and pesticides, also influenced gene expression. In addition, a disc diffusion assay showed that AccCPR24 enhanced the ability of bacterial cells to resist multiple stresses. We infer from our results that AccCPR24 acts in honeybee development and in protecting these insects from abiotic stresses.

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Drosophila cuticular proteins with the R&R Consensus: Annotation and classification with a new tool for discriminating RR-1 and RR-2 sequences

Cited by 150 publications

References 22 publications

Developmental expression patterns of cuticular protein genes with the R&R Consensus from Anopheles gambiae

Developmental expression patterns of cuticular protein genes with the R&R Consensus from Anopheles gambiae

Biopolymers from Animals

CLONING, EXPRESSION PATTERNS, AND PRELIMINARY CHARACTERIZATION OF AccCPR24, A NOVEL RR‐1 TYPE CUTICLE PROTEIN GENE FROM Apis cerana cerana

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