Drosophila Lissencephaly-1 functions with Bic-D and dynein in oocyte determination and nuclear positioning

Swan, Andrew; Nguyen, Thuy N.; Suter, Beat

doi:10.1038/15680

Cited by 210 publications

(197 citation statements)

References 30 publications

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“…One possibility is that the role of dynactin is most important for cargoes, such as individual proteins, that are too small to recruit multiple dyneins. However, the requirement for BICD proteins in the transport of large membrane‐bound cargoes (Swan et al , 1999; Matanis et al , 2002; Larsen et al , 2008; Splinter et al , 2010; Hu et al , 2013) and the involvement of dynactin in most of dynein's functions (Schroer, 2004) suggests that activation of processivity of individual motors is important even when multiple dynein motors are engaged with a cargo.…”

Section: Discussionmentioning

confidence: 99%

In vitro reconstitution of a highly processive recombinant human dynein complex

et al. 2014

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Cytoplasmic dynein is an approximately 1.4 MDa multi‐protein complex that transports many cellular cargoes towards the minus ends of microtubules. Several in vitro studies of mammalian dynein have suggested that individual motors are not robustly processive, raising questions about how dynein‐associated cargoes can move over long distances in cells. Here, we report the production of a fully recombinant human dynein complex from a single baculovirus in insect cells. Individual complexes very rarely show directional movement in vitro. However, addition of dynactin together with the N‐terminal region of the cargo adaptor BICD2 (BICD2N) gives rise to unidirectional dynein movement over remarkably long distances. Single‐molecule fluorescence microscopy provides evidence that BICD2N and dynactin stimulate processivity by regulating individual dynein complexes, rather than by promoting oligomerisation of the motor complex. Negative stain electron microscopy reveals the dynein–dynactin–BICD2N complex to be well ordered, with dynactin positioned approximately along the length of the dynein tail. Collectively, our results provide insight into a novel mechanism for coordinating cargo binding with long‐distance motor movement.

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Section: Discussionmentioning

confidence: 99%

In vitro reconstitution of a highly processive recombinant human dynein complex

et al. 2014

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“…For example, genetic and biochemical analyses in Drosophila have shown an essential role for the protein kinase encoded by misshapen during apical migration of photoreceptor nuclei (Houalla et al, 2005). Misshapen can increase the phosphorylation of Bicaudal-D, a dynein-interacting protein that is also essential for nuclear migration in flies (Suter and Steward, 1991;Swan et al, 1999). The vertebrate homologue of Misshapen is a GCK (Germinal Center Kinase) termed Mink-1 (for Misshapen/Nik-related Kinase-1).…”

Section: Kinases In Nuclear Migrationmentioning

confidence: 99%

Nuclear migration during retinal development

2008

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In this review we focus on the mechanisms, regulation, and cellular consequences of nuclear migration in the developing retina. In the nervous system, nuclear migration is prominent during both proliferative and post-mitotic phases of development. Interkinetic nuclear migration is the process where the nucleus oscillates from the apical to basal surfaces in proliferative neuroepithelia. Proliferative nuclear movement occurs in step with the cell cycle, with M-phase being confined to the apical surface and G1-, S-, and G2-phases occurring at more basal locations. Later, following cell cycle exit, some neuron precursors migrate by nuclear translocation. In this mode of cellular migration, nuclear movement is the driving force for motility. Following discussion of the key components and important regulators for each of these processes, we present an emerging model where interkinetic nuclear migration functions to distinguish cell fates among retinal neuroepithelia. Keywordsinterkinetic nuclear migration; nuclear translocation; nucleokinesis; neurogenesis; dynein; cell cycle; cell behavior OverviewNeuronal development is regulated by a complex array of intrinsic and extrinsic signals that act on progenitor neuroepithelial cells to ensure the correct cell type is generated in the right numbers and at the appropriate time of development (Donovan et al., 2005;Cayouette et al., 2006). In addition, multiple signaling cues are integrated in post-mitotic neuronal precursors to facilitate directed cell migration and correct laminar positioning (Malicki et al., 2004). This spatial and temporal regulation of neuronal cell-type fate commitment and histogenesis is dependent on the regulation of the mitotic cell cycle, and in particular at the level of cell cycle exit (Ohnuma and Harris, 2003). Recent data suggest that nuclear migration is a critical process for several aspects of neuronal development, including that of the neural retina. The focus of this review is on interkinetic nuclear migration and nuclear translocation during retinogenesis, although much of what we know is from observations in other regions of the developing nervous system. Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. NIH Public Access NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author Manuscript Interkinetic nuclear migrationInterkinetic nuclear migration is the proliferative cell behavior where the nuclei of neuroepithelial cells migrate in an apical-basal manner and in phase with the cell cycle (Frade 2002). Neuroepithelial cell divisions are confined to apical locations while S-phase occu...

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“…20 Genetic interaction of LIS1 with the dynein/dynactin/microtubule-mediated pathway has also been suggested from work on early development in Drosophila. [21][22][23] Here, Lis1 was demonstrated to be essential for germ-line division, nuclear positioning and oocyte differentiation much like the dynein heavy chain. Also in Saccharomyces cerevisiae, a LIS1 homolog was found to be involved in nuclear migration.…”

Section: Lis1 and Microtubule Regulationmentioning

confidence: 99%

“…The protein is expressed as a maternal component of the oocyte in both cases 21,58 and embryos homozygous for the mutated allele die as larvae [21][22][23] or following mouse embryo implantation. 59 Furthermore, as seen in heterozygote and compound heterozygote animals it is clear that there is a dose specific response to reduction in LIS1 levels.…”

Section: Lis1 Function In Developmentmentioning

confidence: 99%