Drosophila Met and Gce are partially redundant in transducing juvenile hormone action

Abdou, Mohamed; He, Qianyu; Wen, Di; Zyaan, Ola; Wang, Jing; Xu, Jinjin; Baumann, Aaron A.; Joseph, Justin V.; Wilson, Thomas G.; Li, Sheng; Wang, Jian

doi:10.1016/j.ibmb.2011.09.003

Cited by 155 publications

(222 citation statements)

References 43 publications

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“…effects of allatectomy during larval life (23)(24)(25). Therefore, to determine whether gce had any effect on the maturation of mating behavior, we used a null allele of gce (gce 2.5k ), which is a 2.5 kb deletion in the gce locus that causes loss of gce transcripts (25).…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, to determine whether gce had any effect on the maturation of mating behavior, we used a null allele of gce (gce 2.5k ), which is a 2.5 kb deletion in the gce locus that causes loss of gce transcripts (25). The complete loss of function of gce had no significant effect on the percent mating at 30 h after eclosion, but at 48 h more gce mutant females than CS control females mated (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The duplication of the JH receptor Gce occurred in the higher Diptera (37), and the two have partially redundant functions in the larval fat body of D. melanogaster and at metamorphosis (25). Met plays a distinct role in adult optic lobe development during metamorphosis, whereas Gce has no effect (24,38).…”

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

“…Loss of function of both receptor genes is necessary to mimic the effect of genetic ablation of the CA in larvae, which causes prepupal lethality (23)(24)(25). In the adult, loss of Met causes both delayed and reduced fecundity, whereas loss of gce has only a slight effect on ovarian maturation (25). To elucidate the molecular mechanisms underlying JH's role in the onset of mating in D. melanogaster females, we have genetically ablated the CA in the developing adult and found that the onset of mating behavior was delayed, and this delay could be rescued by JH.…”

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confidence: 99%

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Regulation of onset of female mating and sex pheromone production by juvenile hormone in Drosophila melanogaster

Bilen

Atallah

Azanchi

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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Juvenile hormone (JH) coordinates timing of female reproductive maturation in most insects. In Drosophila melanogaster, JH plays roles in both mating and egg maturation. However, very little is known about the molecular pathways associated with mating. Our behavioral analysis of females genetically lacking the corpora allata, the glands that produce JH, showed that they were courted less by males and mated later than control females. Application of the JH mimic, methoprene, to the allatectomized females just after eclosion rescued both the male courtship and the mating delay. Our studies of the null mutants of the JH receptors, Methoprene tolerant (Met) and germ cell-expressed (gce), showed that lack of Met in Met 27 females delayed the onset of mating, whereas lack of Gce had little effect. The Met 27 females were shown to be more attractive but less behaviorally receptive to copulation attempts. The behavioral but not the attractiveness phenotype was rescued by the Met genomic transgene. Analysis of the female cuticular hydrocarbon profiles showed that corpora allata ablation caused a delay in production of the major female-specific sex pheromones (the 7,11-C27 and -C29 dienes) and a change in the cuticular hydrocarbon blend. In the Met 27 null mutant, by 48 h, the major C27 diene was greatly increased relative to wild type. In contrast, the gce 2.5k null mutant females were courted similarly to control females despite changes in certain cuticular hydrocarbons. Our findings indicate that JH acts primarily via Met to modulate the timing of onset of female sex pheromone production and mating.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Regulation of onset of female mating and sex pheromone production by juvenile hormone in Drosophila melanogaster

Bilen

Atallah

Azanchi

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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Detection of juvenile hormone agonists by a new reporter gene assay using yeast expressing Drosophila methoprene‐tolerant

et al. 2021

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Juvenile hormones (JHs) are sesquiterpenoids that play important roles in the regulation of growth, metamorphosis, and reproduction in insects. Synthetic JH agonists (JHAs) have been used as insecticides and are categorized as a class of insect growth regulators (IGRs). Natural JHs and synthetic JHAs bind to the JH receptor methoprene‐tolerant (Met), which forms a functional JH‐receptor complex with steroid receptor coactivators, such as Drosophila melanogaster Taiman (Tai). The ligand‐bound Met–Tai complex induces the transcription of JH response genes by binding to specific DNA elements referred to as JH response elements (JHREs). In the present study, we established a reporter gene assay (RGA) for detecting natural JHs and synthetic JHAs in a yeast strain expressing D. melanogaster Met and Tai. The yeast RGA system detected various juvenoid ligands in a dose‐dependent manner. The rank order of the ligand potencies of the juvenoids examined in the yeast RGA linearly correlated with those of RGAs for Met–Tai established in mammalian and insect cells. Our new yeast RGA is rapid, easy to handle, cost‐effective, and valuable for screening novel JHAs.

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The juvenile hormone receptor as a target of juvenoid “insect growth regulators”

Jindra

Bittova

2019

Arch Insect Biochem Physiol

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Synthetic compounds that mimic the action of juvenile hormones (JHs) are founding members of a class of insecticides called insect growth regulators (IGRs). Like JHs, these juvenoids block metamorphosis of insect larvae to reproductive adults.Many biologically active juvenoids deviate in their chemical structure considerably from the sesquiterpenoid JHs, raising questions about the mode of action of such JH mimics. Despite the early deployment of juvenoid IGRs in the mid-1970s, their molecular effect could not be understood until recent discoveries of JH signaling through an intracellular JH receptor, namely the ligand-binding transcription factor Methoprenetolerant (Met). Here, we briefly overview evidence defining three widely employed and chemically distinct juvenoid IGRs (methoprene, pyriproxyfen, and fenoxycarb), as agonist ligands of the JH receptor. We stress that knowledge of the target molecule is critical for using these compounds both as insecticides and as research tools.

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Drosophila Met and Gce are partially redundant in transducing juvenile hormone action

Cited by 155 publications

References 43 publications

Regulation of onset of female mating and sex pheromone production by juvenile hormone in Drosophila melanogaster

Regulation of onset of female mating and sex pheromone production by juvenile hormone in Drosophila melanogaster

Detection of juvenile hormone agonists by a new reporter gene assay using yeast expressing Drosophila methoprene‐tolerant

The juvenile hormone receptor as a target of juvenoid “insect growth regulators”

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