Drought-adaptive mechanisms involved in the escape/tolerance strategies of Arabidopsis Landsberg erecta and Columbia ecotypes and their F1 reciprocal progeny

Meyre, David; Leonardi, Agnès; Brisson, G. R.; Vartanian, Nicole

doi:10.1078/s0176-1617(04)70141-8

Cited by 48 publications

(38 citation statements)

References 16 publications

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“…Dry weights were assayed to study the growth performance of the plants during the drought stress period. As previously shown in the work of Meyre et al (2001), we observed a difference in drought stress tolerance between the accessions Ler and Col-0. Col-0 plants acclimate to drought by increasing their root-to-shoot ratio and increasing their water use efficiency, while Ler plants exhibit more of a drought-escape strategy by flowering early and reallocating nutrients from the rosette to the flowers, which leads to a rapid yellowing of rosette leaves.…”

Section: Attre1-overexpressing Lines Are More Tolerant To Severe Drousupporting

confidence: 73%

Overexpression of the Trehalase Gene AtTRE1 Leads to Increased Drought Stress Tolerance in Arabidopsis and Is Involved in Abscisic Acid-Induced Stomatal Closure

Houtte¹,

Vandesteene²,

et al. 2013

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Introduction of microbial trehalose biosynthesis enzymes has been reported to enhance abiotic stress resistance in plants but also resulted in undesirable traits. Here, we present an approach for engineering drought stress tolerance by modifying the endogenous trehalase activity in Arabidopsis (Arabidopsis thaliana). AtTRE1 encodes the Arabidopsis trehalase, the only enzyme known in this species to specifically hydrolyze trehalose into glucose. AtTRE1-overexpressing and Attre1 mutant lines were constructed and tested for their performance in drought stress assays. AtTRE1-overexpressing plants had decreased trehalose levels and recovered better after drought stress, whereas Attre1 mutants had elevated trehalose contents and exhibited a drought-susceptible phenotype. Leaf detachment assays showed that Attre1 mutants lose water faster than wild-type plants, whereas AtTRE1-overexpressing plants have a better water-retaining capacity. In vitro studies revealed that abscisic acidmediated closure of stomata is impaired in Attre1 lines, whereas the AtTRE1 overexpressors are more sensitive toward abscisic acid-dependent stomatal closure. This observation is further supported by the altered leaf temperatures seen in trehalase-modified plantlets during in vivo drought stress studies. Our results show that overexpression of plant trehalase improves drought stress tolerance in Arabidopsis and that trehalase plays a role in the regulation of stomatal closure in the plant drought stress response.

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Section: Attre1-overexpressing Lines Are More Tolerant To Severe Drousupporting

confidence: 73%

Overexpression of the Trehalase Gene AtTRE1 Leads to Increased Drought Stress Tolerance in Arabidopsis and Is Involved in Abscisic Acid-Induced Stomatal Closure

Houtte¹,

Vandesteene²,

et al. 2013

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“…Our work indicates that Col has a greater ability to modify root system morphology in response to osmotic signals than Ler. This is consistent with a study of drought response in Col and Ler, where Col was found to alter development to withstand drought, whereas Ler exhibited drought escape strategies such as early flowering (Meyre et al 2001). Col and Ler provide an interesting system to study the potential benefits or disadvantages of having a small and plastic root system vs. a large root system that is less sensitive to osmotic signals.…”

Section: Colsupporting

confidence: 65%

Identification of Quantitative Trait Loci That Regulate Arabidopsis Root System Size and Plasticity

et al. 2006

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Root system size (RSS) is a complex trait that is greatly influenced by environmental cues. Hence, both intrinsic developmental pathways and environmental-response pathways contribute to RSS. To assess the natural variation in both types of pathways, we examined the root systems of the closely related Arabidopsis ecotypes Landsberg erecta (Ler) and Columbia (Col) grown under mild osmotic stress conditions. We found that Ler initiates more lateral root primordia, produces lateral roots from a higher percentage of these primordia, and has an overall larger root system than Col under these conditions. Furthermore, although each of these parameters is reduced by osmotic stress in both ecotypes, Ler shows a decreased sensitivity to osmotica. To understand the genetic basis for these differences, QTL for RSS under mild osmotic stress were mapped in a Ler 3 Col recombinant inbred population. Two robust quantitative trait loci (QTL) were identified and confirmed in near-isogenic lines (NILs). The NILs also allowed us to define distinct physiological roles for the gene(s) at each locus. This study provides insight into the genetic and physiological complexity that determines RSS and begins to dissect the molecular basis for naturally occurring differences in morphology and developmental plasticity in the root system.

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“…There are numbers of factors which play crucial roles when a plant encounters drought such as plant species and variety, duration and intensity of water deficit,environmental conditions, alterations in water demand from the atmosphere and plant growth as well (Chaves et al, 2002).The result of this study showed that both vte 4 mutant and wild type A.thaliana plants recorded the highest value under control condition consider in gmorphological traits such as rosette dry weight, specific leaf area, rosette dry weight at bolting and root mass fraction in comparison with other water regimes. This result was in accordance with previous study which indicated the fact that by providing the appropriate water availability to the plant, the growth parameters obtained the higher value compared to stress environment (Meyre et al, 2001). In A.thaliana the biomass can be considered by weighing the plant rosette dry weight.…”

Section: Discussionsupporting

confidence: 82%

“…In A.thaliana the biomass can be considered by weighing the plant rosette dry weight. Therefore, Wild type and vte 4 mutant Arabidopsis thaliana responses to different water... the reduction of rosette dry weight and rosette dry weight at bolting under water deficit resulted in biomass decrease (Meyre et al, 2001). In this study the specific leaf area, rosette dry weight and rosette dry weight at bolting were reduced as the frequency of water deficit escalated.…”

Section: Discussionmentioning

confidence: 84%

“…Therefore, the physiological and morphological study of this plant under various oxidative stresses such as water stress can be made possible (Heath et al, 2013) As drought conditions are experienced by plants, different ecotypes of Arabidopsis demonstrate various response strategies, some employing an escape strategy, while other apply a tolerance strategy (Meyre et al, 2001). Considering different response strategies affecting growth and development of the plants, the type of the plant must be taken into consideration when analyzing results (Meyre et al, 2001 (Havaux et al, 2005). Hence, studying the response of mutant plants (vte) compared to wild type of A.thaliana under varied water stress seems crucial and will provide important information on mechanism of the plants against this abiotic stress.…”

Section: Arabidopsis Thaliana Is a Widely Used Model Organismmentioning

confidence: 99%

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Wild type and vte4 mutant Arabidopsis thaliana responses to different water frequencies: genetic engineering towards stress tolerance

Khalatbari

Jaafar

Mahmood

et al. 2015

J. Soil Sci. Plant Nutr.

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Growth and development of plants are severely affected once exposed to soil water deficit. In addition, plants experience different levels of water stress in which their adaptation and response will be different. In this paper, we sought to investigate the growth, development and water relations of both wild-type (Col-0) and vte 4 mutant Arabidopsis thaliana under different water frequencies. These water treatments, including control condition, 4 days of water withholding and 8 days of water withholding were imposed on the plants. Each water treatment was replicated three times in a complete randomized design with factorial arrangement. Wild type and mutant A.thaliana plants were subjected to the abiotic stress (water stress) for up to 24 days. The results indicated that under water stress, the performance of wild type plants were stronger than vte 4 mutants. Under control condition, specific leaf area, rosette dry weight and rosette dry weight at bolting of wild type A.thaliana scored the highest values of 47.66 mm²/mg, 13.67 mg and 201.5 mg, respectively in comparison with vte 4 mutant. However, both wild type and vte 4 mutant plants were negatively affected as the water treatments continued. The root mass fraction showed an increase in vte 4 mutant and wild type A.thaliana as they scored 0.136g/g and 0.17 g/g, respectively. Under the same treatment, water potential indicated a reduction for both plan types where vte 4 and wild type plants obtained the values of -1.4 and -1.3 MPa, respectively after 24 days of stress initiation. As a result, our findings suggested that different water treatments significantly differed in growth characters in which the absence of γ-tocopherol methyltransferase (γ -TMT) gene in vte 4 had an impact on the plant's response towards the water deficit.

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Drought-adaptive mechanisms involved in the escape/tolerance strategies of Arabidopsis Landsberg erecta and Columbia ecotypes and their F1 reciprocal progeny

Cited by 48 publications

References 16 publications

Overexpression of the Trehalase Gene AtTRE1 Leads to Increased Drought Stress Tolerance in Arabidopsis and Is Involved in Abscisic Acid-Induced Stomatal Closure

Overexpression of the Trehalase Gene AtTRE1 Leads to Increased Drought Stress Tolerance in Arabidopsis and Is Involved in Abscisic Acid-Induced Stomatal Closure

Identification of Quantitative Trait Loci That Regulate Arabidopsis Root System Size and Plasticity

Wild type and vte4 mutant Arabidopsis thaliana responses to different water frequencies: genetic engineering towards stress tolerance

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