Drought tolerance depends on the age of the spring wheat seedlings and differentiates patterns of proteinases

Chl a fluorescence and proteomics reveal protection of the photosynthetic apparatus to dehydration in tolerant but not in susceptible wheat cultivars

“…The 11-d-old spring wheat seedlings were used for studies because at this developmental stage, drought tolerance, characteristic for germination, is lost (Miazek et al 2017). Additionally, that stage can be critical for yield in field conditions due to possible inhibition of tillering.…”

“…Ethos and Zebra differing in drought resistance were placed side by side between double filter paper strips moistened with a Knopp solution supplemented with Hoagland micronutrients (Grudkowska and Zagdanska 2010), rolled up, and grown for 11 d in plastic boxes in a climate chamber with day/night temperatures of 18/14 C, a 16-h photoperiod, a photosynthetic photon flux density (PPFD) of 260 µmol m -2 s -1 , and an air humidity of 70 -80 %. To induce dehydration, the nutrient solution was drained for 24 -96 h. The water saturation deficit (WSD) in leaves was determined gravimetrically as described in details by Miazek et al (2017). Fully rigid, control seedlings and seedlings having WSD of 15, 30, and 50 % were used for following studies.…”

Machine learning in determination of water saturation deficit in wheat leaves on basis of Chl a fluorescence parameters

“…WSD refers to the degree of tissues dehydration, phenomenon which occurs as one of the first symptoms of plant responses to various environmental stimuli, such as soil salinity, both drought, and flooding, and temperature over or under optimal ranges (Grudkowska and Zagdańska 2010). Plant wilting is an easy to notice as a physiological reaction, associated with complex biochemical processes leading to induction of the stress tolerance or the programmed cell death occurring under extreme situations in tissues of stress-susceptible plants (Kosová et al 2015, Miazek et al 2017. WSD fluctuations as a simple phenotypic trait meets the requirements for being an indicator of plant stress tolerance (Noctor et al 2015, Gietler et al 2016, Miazek et al 2017.…”

“…Plant wilting is an easy to notice as a physiological reaction, associated with complex biochemical processes leading to induction of the stress tolerance or the programmed cell death occurring under extreme situations in tissues of stress-susceptible plants (Kosová et al 2015, Miazek et al 2017. WSD fluctuations as a simple phenotypic trait meets the requirements for being an indicator of plant stress tolerance (Noctor et al 2015, Gietler et al 2016, Miazek et al 2017. Unfortunately, mere WSD detection does not meet requirements of the screening test due to the low bandwidth.…”

“…WSD and corresponding Chl a fluorescence data were collected as part of programmes on the effects of water shortages on crop yields, run in (Stefański et al 2018). In all experiments, the WSD was determined gravimetrically in three replicas (Miazek et al 2017).…”