Drought tolerance selection of soybean lines generated from somatic embryogenesis using osmotic stress simulation of polyethylene glycol (PEG)

Abstract

Abstract. Sunaryo W, Widoretno W, Nurhasanah, Sudarsono. 2016. Drought tolerance selection of soybean lines generated from somatic embryogenesis using osmotic stress simulation of poly-ethylene glycol (PEG). Nusantara Bioscience 8: 45-54. Somaclonal variation is an alternative source to create genetic variability including generating a novel character like drought tolerance. The objective of this research was to select the drought tolerant lines derived from somatic embryogenesis (somaclones) of 4 soybean genotypes using drought stress simulated by Polyethylene Glycol (PEG). Soybean seeds (R 1 generation) generated from 37 somaclones (R 0 generation) were grown on semi hydroponic system using Greenleaf as supporting medium. The seedlings were watered with half strength of liquid Murishage and Skoog nutrient. The PEG solution treatment of either 0% or 15% (PEG: w/v = -0.41 Mpa osmotic potential) was applied in the MS solution, from the seedlings of 14 days old until harvesting period. The vegetative growth, intensity of leaf firing, plant biomass, and drought sensitivity index variables were observed during the PEG application. The same procedure was applied to the seeds, propagated conventionally, from the same genotypes as controls. The results showed that somatic embryogenesis had altered the drought sensitivity of soybean genotypes under drought stress simulated by PEG. Interestingly, the different drought tolerance level was showed by the R 1 plants. Some lines were increased and the other lines were decreased compared to the control genotypes. From a total of 185 R 1 plants, 4 plants increased their tolerance against drought stress and grouped as tolerant genotype.

Cell Suspension Culture and In Vitro Screening for Drought Tolerance in Soybean Using Poly-Ethylene Glycol

“…Use of PEG or mannitol during in vitro selection for identification of abiotic stress-tolerant tissues has been reported earlier in potato and rice [ 8 , 12 , 13 ]. Various reports are available on use of PEG for successful in vitro selection for drought tolerance in maize [ 6 ], pelargonium [ 14 ], banana [ 15 ], sorghum [ 16 ], peanut [ 17 ] and soybean [ 2 , 18 ]. However, to achieve such a goal, a competent and reproducible plant regeneration method must be accessible from callus and cell suspension cultures.…”

Polyethylene Glycol (Peg) Mediated In Vitro Characterization of Sugarcane (CP-77/400) Calli and Regenerated Plantlets

“…Over the past few decades, tissue culture techniques have been effectively used to develop stress tolerance in crop plants. Drought tolerant cell lines were developed in several plants species through in vitrosomaclonal variation such as sunflower (Hassan et al, 2004), soybean (Sunaryo et al, 2016), mung bean (Gulati and Jaiwal, 1993), tomato (Kulkarni and Deshpande, 2007), maize (Matheka et al, 2008), rice (Wani et al, 2010) and sugarcane (Begum et al, 2011).…”

RAPD Analysis for Genetic Variability Detection of Mutant Soybean (<i>Glycine max</i> (L.) Merr)

“…This method utilizes the polyethylene glycol (PEG) as an osmotic solution. Somotoclone method has been proven to derived new varieties of soybean that tolerance against drought stress (Sunaryo et al 2016). However, this technology requires a high cost and advance tool that makes it difficult to be implemented by the farmer.…”