Drought-Tolerant Brassica rapa Shows Rapid Expression of Gene Networks for General Stress Responses and Programmed Cell Death Under Simulated Drought Stress

Guo, Yi; Samans, Birgit; Chen, Sheng; Kibret, Kidist B.; Hatzig, Sarah; Turner, Neil C.; Nelson, Matthew N.; Cowling, Wallace; Snowdon, Rod J.

doi:10.1007/s11105-017-1032-4

Cited by 33 publications

(26 citation statements)

References 86 publications

(104 reference statements)

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“…B. rapa is distributed widely on a global scale 2 Plant Phenomics with the centre of origin in the Old World and centres of diversity in Asia [10][11][12][13][14], with some types flourishing in heat and drought-affected regions [15]. Genotypic variation for heat and drought resistance has been reported recently in B. rapa [16,17], and a tolerant genotype responded to simulated drought with rapid expression of gene networks for general stress responses and programmed cell death [18].…”

Section: Introductionmentioning

confidence: 99%

Nondestructive Phenomic Tools for the Prediction of Heat and Drought Tolerance at Anthesis in Brassica Species

et al. 2019

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Oilseed Brassica species are vulnerable to heat and drought stress, especially in the early reproductive stage. We evaluated plant imaging of whole plant and flower tissue, leaf stomatal conductance, leaf and bud temperature, photochemical reflectance index, quantum yield of photosynthesis, and leaf gas exchange for their suitability to detect tolerance to heat (H) and/or drought (D) stress treatments in 12 Brassica genotypes (G). A replicated factorial experiment was set up with 7 d of stress treatment from the beginning of anthesis with various levels of three factors H, D, and G. Most phenomics tools detected plant stress as indicated by significant main effects of H, D, and H×D. Whole plant volume was highly correlated with fresh weight changes, suggesting that whole plant imaging may be a useful surrogate for fresh weight in future studies. Vcmax, the maximum carboxylation rate of photosynthesis, increased rapidly on day 1 in H and H+D treatments, and there were significant interactions of G×H and G×D. Vcmax of genotypes on day 1 in H and H+D treatments was positively correlated with their harvested seed yield. Vcmax on day 1 and day 3 were clustered with seed yield in H and H+D treatments as shown in the heatmaps of genotypic correlations. TPU, the rate of triose phosphate use, also showed significant positive genotypic correlations with seed yield in H+D treatments. Flower volume showed significant interactions of G×H and G×D on day 7, and flower volume of genotypes on day 7 in H was positively correlated with their harvested seed yield. There were few interactions of G×H or G×D for leaf stomatal conductance, leaf and bud temperature, photochemical reflectance index, and quantum yield of photosynthesis. Vcmax, TPU, and volume of flowers are potential nondestructive phenomic traits for heat or combined heat and drought stress tolerance screening in Brassica germplasm.

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Section: Introductionmentioning

confidence: 99%

Nondestructive Phenomic Tools for the Prediction of Heat and Drought Tolerance at Anthesis in Brassica Species

et al. 2019

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“…Also, these studies found that JA is required for resistance, indicated by susceptibility in mutants with non-functional copies of genes involved in the JA pathway, including a mutation in the downstream COI1 gene (see Glazebrook 2005 for further details). Additionally, a recent B. rapa expression study was used to identify candidate drought response genes (Guo et al, 2017).…”

Section: Plant-pathogen Systemmentioning

confidence: 99%

“…We next considered 7,076 genes candidate drought response genes (Additional le 3) which were differentially expressed in Guo et al (2017) study in response to a polyethylene glycol (PEG) treatment, as compared to controls (no PEG), and that had a >2 fold change at 12 hours post-treatment. We then identi ed genes differentially expressed in common among both this drought study and the NFPR studies (above).…”

Section: Functional Analysismentioning

confidence: 99%

Evolution of pathogen response genes associated with increased disease susceptibility during adaptation to an extreme drought in a Brassica rapa plant population

O’Hara

Franks

Kane

et al. 2020

Preprint

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BACKGROUND Pathogens are key components in natural and agricultural plant systems. There is evidence of evolutionary changes in disease susceptibility as a consequence of climate change, but we know little about the underlying genetic basis of this evolution. To address this, we took advantage of a historical seed collection of a Brassica rapa population, which we previously demonstrated evolved an increase in disease susceptibility to a necrotrophic fungal pathogen following a drought. RESULTS Previously, we combined a resurrection experiment with genome-wide sequencing of 124 pooled ancestral and descendant plants. Here, using these previously generated sequence data (Franks et al, 2016), we show that well-characterized necrotrophic fungal pathogen response (NFPR) genes have evolved, as indicated by changes in allele frequency, between ancestors and descendants, with several of them identified as extreme FST outliers. The jasmonic acid (JA) signaling pathway in particular seems to underlie the evolution of disease susceptibility, in addition to its well characterized role in plastic disease response. We identify a list of 260 genes that are both NFPR genes and are differentially expressed in response to drought, based on publicly available data. We present evidence that five of these genes evolved between ancestors and descendants, suggesting that the drought acted as the evolutionary driver, and that the accompanying increase in disease susceptibility may have been a consequence of genetic pleiotropy. CONCLUSIONS Our study provides evidence that for this population, standing variation in NFPR genes is affected by natural selection related to climate change. Our results reveal potentially important candidates that may underlie trait evolution in both crops and natural systems. Additionally, this trade-off between adaptation to biotic and abiotic stresses is an example of how climate change can have diverse and unexpected consequences.

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“…To study the expression pattern of BrGRP genes under drought stress, the transcriptome data of Chinese cabbage (CR2355 and ATC92037) were also analyzed [29]. CR2355 is a root transcriptome data of drought-tolerant (DT) material which can keep the required biomass for mature when suffered from transient drought stress during the reproductive phase, while ATC92037 is a root transcriptome data of drought-sensitive (DS) material which showed a significant reduction in biomass after transient drought stress.…”

Section: Expression Profiling Of Brgrps Under Drought Stressmentioning

confidence: 99%

“…The expression differences of BrGRP genes under biotic and abiotic stress were also analyzed based on the transcriptome data of Chinese cabbage under high temperature at 45 ℃ [27], low temperature at 4 ℃ [28], drought [29], soft rot stress [30]. The expression levels of BrGRP genes were calculated with the Fragments Per kb per Million reads (FPKM) values [55] and displayed with cluster heat map drawn by TBtools [52].…”

Section: Expression Pattern Analysis Of Brgrp Genesmentioning

confidence: 99%

Genome-wide identification and expression analysis of glycine-rich protein genes in Chinese cabbage (Brassica rapa L. ssp. pekinensis)

Gao

Cheng

et al. 2019

Preprint

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Background Plant Glycine-rich proteins, a superfamily with a glycine-rich domain, play an important role in various stress such as low temperature, drought, high salt, and so on. Although the research of GRP genes has been reported in many plants, the GRP gene has seldom reported in Chinese cabbage so far. Research results made a guide to further understand the function of BrGRP genes in Chinese cabbage. Results In this study, a total of 141 glycine-rich protein genes were identified in Chinese cabbage by homology comparative analysis. A further prediction of physical and chemical characteristics revealed that 58.3% of BrGRPs were alkalines, 63.1% of BrGRPs were unstable, and 73.8% were hydrophilic. Conserved domain analysis showed that 110 BrGRPs contained 18 same conserved motifs, and could be classified into five main subclasses which the evolutionary relationship and gene structure may be conserved while the other 31 BrGRPs, including Bra014168 , Bra040002 , etc, may gain new functions or gradually lost gene functions according to the evolution process. These identified BrGRP genes were also located in ten chromosomes and three different subgenomes of Chinese cabbage, and 101 pairs of orthologous GRP genes were found between Chinese cabbage and Arabidopsis. According to the opened transcriptome data, we found that 138 BrGRP genes showed abnormal expression at high temperature, 108 BrGRP genes showed abnormal expression at low temperature, and 74 at drought stress, 47 at soft rot stress, while only 3 and 7 genes at ozone and salt stress, respectively. Further promoter motif analysis found that a large number of stress-related cis-acting elements, such as DRE, MYC, MYB, and ABRE, were identified in their promoter regions, which were in correspondence with previous differential expression. In addition, some BrGRP genes were involved in multiple stresses suggested their broad-spectrum resistance. Conclusion A total of 141 GRP genes were identified in Chinese cabbage, which suggested their potential roles in plant stress response. But the molecular mechanisms by which BrGRP genes respond and resist biotic and abiotic stress remain unclear. These results may provide an important basis for the study of their function in Chinese cabbage.

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Drought-Tolerant Brassica rapa Shows Rapid Expression of Gene Networks for General Stress Responses and Programmed Cell Death Under Simulated Drought Stress

Cited by 33 publications

References 86 publications

Nondestructive Phenomic Tools for the Prediction of Heat and Drought Tolerance at Anthesis in Brassica Species

Nondestructive Phenomic Tools for the Prediction of Heat and Drought Tolerance at Anthesis in Brassica Species

Evolution of pathogen response genes associated with increased disease susceptibility during adaptation to an extreme drought in a Brassica rapa plant population

Genome-wide identification and expression analysis of glycine-rich protein genes in Chinese cabbage (Brassica rapa L. ssp. pekinensis)

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