Drug-Free Chitosan Coated Poly(isobutylcyanoacrylate) Nanoparticles Are Active Against Trichomonas vaginalis and Non-Toxic Towards Pig Vaginal Mucosa

Pradines, Bénédicte; Bories, Christian; Vauthier, Christine; Ponchel, Gilles; Loiseau, Philippe M.; Bouchemal, Kawthar

doi:10.1007/s11095-014-1528-7

Cited by 41 publications

(33 citation statements)

References 28 publications

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“…This confirms chitosan as a superior component in our vaginal drug delivery system since chitosan is expected to disrupt the biofilm allowing the vesicle-associated antibacterial agent to reach the bacteria that would otherwise be shielded by the biofilm. Importantly, chitosan has also been reported to act against Trihomonas vaginalis infections, further strengthening its potential in antimicrobial vaginal therapy [47]. …”

Section: Resultsmentioning

confidence: 99%

Chitosan-Based Nanomedicine to Fight Genital Candida Infections: Chitosomes

et al. 2017

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Vaginal infections are associated with high recurrence, which is often due to a lack of efficient treatment of complex vaginal infections comprised of several types of pathogens, especially fungi and bacteria. Chitosan, a mucoadhesive polymer with known antifungal effect, could offer a great improvement in vaginal therapy; the chitosan-based nanosystem could both provide antifungal effects and simultaneously deliver antibacterial drugs. We prepared chitosan-containing liposomes, chitosomes, where chitosan is both embedded in liposomes and surface-available as a coating layer. For antimicrobial activity, we entrapped metronidazole as a model drug. To prove that mucoadhesivness alone is not sufficient for successful delivery, we used Carbopol-containing liposomes as a control. All vesicles were characterized for their size, zeta potential, entrapment efficiency, and in vitro drug release. Chitosan-containing liposomes were able to assure the prolonged release of metronidazole. Their antifungal activity was evaluated in a C. albicans model; chitosan-containing liposomes exhibited a potent ability to inhibit the growth of C. albicans. The presence of chitosan was crucial for the system’s antifungal activity. The antifungal efficacy of chitosomes combined with antibacterial potential of the entrapped metronidazole could offer improved efficacy in the treatment of mixed/complex vaginal infections.

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Section: Resultsmentioning

confidence: 99%

Chitosan-Based Nanomedicine to Fight Genital Candida Infections: Chitosomes

et al. 2017

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“…3). The 121 cytotoxicity is thus independent on the surface charge properties 122 of the nanoparticles since PIBCA/F68 nanoparticles are negatively-123 charged, while chitosan-coated nanoparticles are positively-124 charged(Pradines et al, 2015). According to the results inFig.…”

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confidence: 95%

“…45Nanoparticles were prepared by the anionic emulsion poly-46 merization of isobutylcyanoacrylate monomer according toPradines et al (2015). Briefly, 0.069 g of mixture of chitosan and 48 thiolated chitosan were dissolved in 5 mL of 0.calf serum and 1% amino-acid non-essential.…”

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confidence: 99%

Cell line-dependent cytotoxicity of poly(isobutylcyanoacrylate) nanoparticles coated with chitosan and thiolated chitosan: Insights from cultured human epithelial HeLa, Caco2/TC7 and HT-29/MTX cells

Pradines

Moal

Vauthier

et al. 2015

International Journal of Pharmaceutics

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“…Pluronic F127 has been approved by the FDA for use as a food additive and pharmaceutical ingredient. Moreover, Pluronic F127 hydrogel showed noncytotoxicity toward pig vaginal mucosa (30), indicating the biocompatibility of the Pluronic F127-based hydrogels reported here.…”

Section: Resultsmentioning

confidence: 53%

Thermosensitive and Mucoadhesive Pluronic-Hydroxypropylmethylcellulose Hydrogel Containing the Mini-CD4 M48U1 Is a Promising Efficient Barrier against HIV Diffusion through Macaque Cervicovaginal Mucus

Bouchemal

Aka-Any-Grah

Dereuddre‐Bosquet

et al. 2015

Antimicrob Agents Chemother

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iTo be efficient, vaginal microbicide hydrogels should form a barrier against viral infections and prevent virus spreading through mucus. Multiple particle tracking was used to quantify the mobility of 170-nm fluorescently labeled COOH-modified polystyrene particles (COOH-PS) into thermosensitive hydrogels composed of amphiphilic triblock copolymers with block compositions EO n -PO m -EO n (where EO refers to ethylene oxide and PO to propylene oxide) containing mucoadhesive hydroxypropylmethylcellulose (HPMC). COOH-PS were used to mimic the size and the surface charge of HIV-1. Analysis of COOH-PS trajectories showed that particle mobility was decreased by Pluronic hydrogels in comparison with cynomolgus macaque cervicovaginal mucus and hydroxyethylcellulose hydrogel (HEC; 1.5% by weight The idea exposed in this work is to form a physical barrier composed of thermosensitive and mucoadhesive hydrogel against HIV diffusion, thus limiting virus attachment to mucosal surfaces of the vagina. The thermosensitive property of the (ethylene oxide) 98 (propylene oxide) 67 (ethylene oxide) 98 (EO 98 PO 67 EO 98 ) block copolymer designated Pluronic F127 or poloxamer P407 is particularly interesting for the design of vaginal microbicides acting as a physical barrier. At a certain concentration, this system is fluid at room temperature and in the form of a gel at body temperature (37°C) (1). In contrast to semisolid hydrogels, the administration of the formulation in the form of a solution (for instance, via syringes or another suitable device) facilitates its spreading on the mucosa, while the hydrogel layer on the mucosal surface could allow the formation of a physical barrier against virus diffusion. Mucoadhesion of Pluronic hydrogels was further improved by adding hydroxypropylmethylcellulose (HPMC) as a mucoadhesive polymer (1).Previous works reported different techniques for the investigation of this barrier effect by using viruses or virus-like particles.Diffusion chambers and Transwell-Snapwell chambers were used for hydrogel samples. The principle is based on donor-receptor duality. The sample containing the tracking substance, such as polystyrene particles, is placed in a donor chamber, and their passage in a receiver chamber filled with an appropriate liquid medium is observed (2, 3). However, this technique did not allow control of parameters such as thickness and uniformity of the sample layer, influence of preparation and handling on the structure of the sample, and optimal diameter of the pore to prevent blockage with the hydrogel sample (4). Fluorescence-labeled probe observations using fluorescence recovery after photobleaching (FRAP), single or multiple particle tracking (SPT or

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Drug-Free Chitosan Coated Poly(isobutylcyanoacrylate) Nanoparticles Are Active Against Trichomonas vaginalis and Non-Toxic Towards Pig Vaginal Mucosa

Abstract: This study demonstrated that poly(isobutylcyanoacrylate) nanoparticles coated with chitosan were active against T. vaginalis without adding a drug. Besides their anti-T. vaginalis activity, the formulations are non-toxic towards pig vaginal mucosa.

Cited by 41 publications

References 28 publications

Chitosan-Based Nanomedicine to Fight Genital Candida Infections: Chitosomes

Chitosan-Based Nanomedicine to Fight Genital Candida Infections: Chitosomes

Cell line-dependent cytotoxicity of poly(isobutylcyanoacrylate) nanoparticles coated with chitosan and thiolated chitosan: Insights from cultured human epithelial HeLa, Caco2/TC7 and HT-29/MTX cells

Thermosensitive and Mucoadhesive Pluronic-Hydroxypropylmethylcellulose Hydrogel Containing the Mini-CD4 M48U1 Is a Promising Efficient Barrier against HIV Diffusion through Macaque Cervicovaginal Mucus

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