Drug loaded composite oxidized pectin and gelatin networks for accelerated wound healing

Tummalapalli, Mythili; Berthet, Morgane; Verrier, Bernard; Deopura, B. L.; Alam, Mohammad Sarwar; Gupta, Bhuvanesh

doi:10.1016/j.ijpharm.2016.04.007

Cited by 64 publications

(27 citation statements)

References 46 publications

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“…To repair large skin wounds, there is the tissue engineering approach available, which is based on a combination of a wide variety of synthetic and biological materials such as collagen, chitosan, poly(glycerol sebacate) (PGS), polyurethane, and in vitro‐cultured cells, as well as employing new techniques of fabricating scaffolds that mimic the structure and function of extracellular matrix (ECM) of tissues. In recent years, wound dressings have made tremendous progress, which closely resemble the normal skin and meet all the requirements of stimulating wound healing phases with minimum negative effects, such as the formation of scar tissue . Ayati Najafabadi et al produced a wound dressing based on oxidized pectin and gelatin (Gel) with ability to release drug .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of sustained ciprofloxacin release of biodegradable electrospun gelatin/poly(glycerol sebacate) mat membranes for wound dressing applications

Najafabadi

Shirazaki

Kharazi

et al. 2018

Asia-Pacific J Chem Eng

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In this research, gelatin and poly(glycerol sebacate) (PGS), which have demonstrated significantly developed wound healing process, were used to fabricate biodegradable and bioabsorbable membranes with controlled release of ciprofloxacin (CIP) as a powerful antibiotic. This was achieved by taking advantage of electrospinning technique to accelerate the wound healing as an extracellular matrix-liked composite membrane. We attempted to carefully investigate the impact of variable ratio of sebacic acid and glycerol as components of PGS and identified CIP-loaded cross-linked membrane (CIP-M 11 ) as an optimum membrane, although a significant change in structural properties of samples was not observed. The physicochemical properties of drug-loaded electrospun wound cover were evaluated by several characterization tests.Scanning electron microscopy and Fourier transform infrared spectroscopy results confirmed that CIP-embedded gelatin/PGS membrane was successfully synthesized and cross-linked properly. The CIP-M 11 sample showed a constant and uniform degradation rate with the capacity of durable drug release over the time period. The excellent antibacterial activity of CIP decreased the population of both Escherichia coli and Staphylococcus aureus bacteria and prevented bacterial infections. The cytocompatibility of membranes was completely proven by culturing L929 fibroblast cells on the prepared membranes. Finally, obtained results exhibit that the membranes should be considered as the promising wound dressing.

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Section: Introductionmentioning

confidence: 99%

Evaluation of sustained ciprofloxacin release of biodegradable electrospun gelatin/poly(glycerol sebacate) mat membranes for wound dressing applications

Najafabadi

Shirazaki

Kharazi

et al. 2018

Asia-Pacific J Chem Eng

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“…Many formulations for the topical delivery of antimicrobials, for example in the context of wound care involve hydrophylic (bio)polymers such as gelatin, in the form of gelatin nanofibers . Also gelatin can be used mixing with natural or synthetic polymeric systems to produce wound dressings like gelatin‐dendrimer conjugate nanofiber using polyamidoamine (PAMAM) dendrimer G3.5 with gelatin (porcine skin‐ type A) developed either drug delivery and wound care . Besides natural gelatins, candidate hydrophilic biopolymers for such applications certainly also include protein polymers such as hydrophilic protein polymers with collagen‐like GXY repeats …”

Section: Discussionmentioning

confidence: 99%

Fusion of plectasin derivative NZ2114 with hydrophilic random coil polypeptide: Recombinant production in Pichia pastoris and antimicrobial activity against clinical strain MRSA

et al. 2018

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One of the roadblocks towards the practical use of antimicrobial peptides for medical use is their relatively high cost when synthesized chemically. Effective recombinant production has only been successful in some cases, such as the previously reported production in Pichia pastoris of the antimicrobial plectasin derivative peptide NZ2114. The same production host has also been used extensively to produce so-called protein-polymers: sequences that consist of repetitions of simple amino acid motifs found in structural proteins such as collagen and elastin, and that can be designed to self-assemble in micelles, fibers and hydrogels. With the eventual goal of producing recombinant biomaterials such as antimicrobial protein polymer, we here explore the secreted production in Pichia pastoris of a fusion of NZ2114 with a hydrophilic random coil protein polymer C P 4 . The intact NZ2114-C P 4 fusion copolymer was produced with a yield of purified protein in the order of 1 g L 21 supernatant. We find that purified NZ2114-C P 4 has an activity against clinical strain MRSA, but very much lower than activity of chemically synthesized NZ2114. We conclude that possibly, the activity of NZ2114 is impaired by the C-terminal attachment to the protein polymer chain, but other reasons for the low activity cannot yet be excluded either. K E Y W O R D Santimicrobial peptides, MRSA, Pichia pastoris, plectasin NZ2114

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“…Drug release behaviour was examined as per the method described by Teng et al Typically, 10 mg of ciprofloxacin‐loaded nanosoy was weighed and dispersed in 20 mL of release buffer (phosphate‐buffered saline (PBS), pH = 7.4) at 37 °C with mild shaking for 0.5–20 h. Subsequent centrifugation was performed at 10000 rpm for 20 min which led to precipitation of nanosoy. The absorption maximum of the supernatant containing released ciprofloxacin was measured at a wavelength of 268 nm using a Shimadzu UV‐2450 spectrophotometer (Shimadzu, Singapore) against PBS as blank . The precipitate was then disposed of, and a fresh aliquot was prepared for another measurement.…”

Section: Methodsmentioning

confidence: 99%

Size‐controlled preparation of nanosoy for potential biomedical applications

Singh

Gupta

2016

Polymer International

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Soy protein isolate nanoparticles (nanosoy) have significant applications in drug delivery, wound care systems and tissue engineering. We report an optimum technique named nanoprecipitation which enabled one-step formation of near-monodisperse nanosoy based on solvent displacement. Excellent control over protein aggregation was achieved, producing nanoparticles in the size range of 5-15 nm. The effect of various process parameters such as temperature, solvent/non-solvent ratio, crosslinker content and type of surfactant on the particle size was investigated. The structural and morphological features were analysed using dynamic light scattering, X-ray diffraction and high-resolution transmission electron microscopy. The morphological examination indicated that the particles formed were spherical in shape and further reduction in the average particle diameter was achieved by reducing the temperature and solvent/non-solvent ratio of the reaction medium. Surface charge and stability of nanosoy dispersions were analysed using zeta potential measurements. Further, ciprofloxacin release was monitored using nanosoy as a drug carrier. This technique provides an effective and straightforward approach offering considerable advantages in terms of economic, technical and environmental performance.

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Drug loaded composite oxidized pectin and gelatin networks for accelerated wound healing

Cited by 64 publications

References 46 publications

Evaluation of sustained ciprofloxacin release of biodegradable electrospun gelatin/poly(glycerol sebacate) mat membranes for wound dressing applications

Evaluation of sustained ciprofloxacin release of biodegradable electrospun gelatin/poly(glycerol sebacate) mat membranes for wound dressing applications

Fusion of plectasin derivative NZ2114 with hydrophilic random coil polypeptide: Recombinant production in Pichia pastoris and antimicrobial activity against clinical strain MRSA

Size‐controlled preparation of nanosoy for potential biomedical applications

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