Drug release, cell adhesion and wound healing evaluations of electrospun carboxymethyl chitosan/polyethylene oxide nanofibres containing phenytoin sodium and vitamin C

Zarandi, Mohammad Amin; Zahedi, Payam; Rezaeian, Iraj; Salehpour, Alireza; Gholami, Mahdi; Motealleh, Behrooz

doi:10.1049/iet-nbt.2014.0030

Cited by 54 publications

(22 citation statements)

References 38 publications

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“…Previously, investigators have demonstrated that the inclusion of AA in culture media can enhance keratocyte growth and endogenous collagen synthesis in glutaraldehyde crosslinked collagen‐glycosaminoglycan scaffolds . Moreover, AA released from electrospun carboxymethyl chitosan/polyethylene oxide nanofibers exhibited notable efficacy in wound closure owing to the accumulation of AA‐induced collagenous matrix and the regeneration of the epidermis layer . In accordance with these earlier findings, our results further demonstrate that by selecting a proper dendrimer generation, the resulting thermogel can possess high biocompatibility and the capability to release AA molecules at an appropriate rate, consequently enhancing collagen production in cell cultures.…”

Section: Resultssupporting

confidence: 90%

Dendritic Effects of Injectable Biodegradable Thermogels on Pharmacotherapy of Inflammatory Glaucoma‐Associated Degradation of Extracellular Matrix

Nguyen

Luo

Lai

2019

Adv Healthcare Materials

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111.8 million people worldwide by 2040. [1] High intraocular pressure (IOP) has been considered the major indicator for diagnosing glaucoma, and the reduction of elevated IOP is the current clinical approach used to prevent disease progression. [2] Several models of glaucoma have indicated that inflammatory components can directly link elevated IOP with retinal degeneration. [3][4][5][6] As a result, the expression of inflammatory molecules, including nitric oxide, [7] interleukins (ILs), [8] p38 MAPK, [9] and prostaglandin E2 (PGE2), [10] are increased in progressive glaucoma. Furthermore, inflammatory progression is also associated with the increased expression of matrix metalloproteinases (MMPs), [11] which consequently induce the degradations of extracellular matrix (ECM) components such as stromal collagen [12] and retinal laminin. [13] It is noteworthy that ECM possesses adhesive characteristics that can regulate cell function and survival; changes in composition and status of ECM components can result in disruptions of cell-cell and cell-ECM interactions, consequently inducing cell apoptosis. [14][15][16] Therefore, it is highly imperative to develop new therapies for effectively attenuating the upregulation of inflammatory molecules as well as the degradation of stromal collagen and retinal laminin in glaucoma.Dendrimers, which are synthetic macromolecules with well-defined 3D shapes that contain a central core, repeating branched units, and dense shells with terminal functional groups, have been emerging in polymeric architecture in biomedical engineering. These macromolecules can increase the water solubility, bioavailability, and biocompatibility of pharmaceutical agents and show promise for practical use as drug carriers. [17] More interestingly, dendrimers can also be exploited as a key component that regulates the encapsulation and release of other carriers to achieve improved drug delivery. For example, the interior cavities of polyamidoamine (PAMAM) dendrimers can entrap drug molecules, while their amine-terminated surface groups are available for crosslinking with poly(ethylene glycol) mono/diacrylate to generate hydrogel materials with the ability to release drugs in a sustained manner through a twostage process, which involves the release of drug-entrapping dendrimers from degradable hydrogels and the release of drug The development of advanced drug delivery systems with extensively sustained release and multiple functions is highly imperative for effective attenuation of the degradation of ocular extracellular matrix that is associated with inflammatory glaucoma. Here, the generation of amine-terminated polyamidoamine dendrimers in an injectable biodegradable thermogel is demonstrated to be important for achieving prolonged drug release profiles and potent anti-inflammatory effects. Among various generations (Gx, x = 0, 1, 3, 5), third-generation G3 is proved as the most effective material for optimizing the synergistic effects of gelatin and poly(N-isopropylacrylamide) and generating a thermo...

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Section: Resultssupporting

confidence: 90%

Dendritic Effects of Injectable Biodegradable Thermogels on Pharmacotherapy of Inflammatory Glaucoma‐Associated Degradation of Extracellular Matrix

Nguyen

Luo

Lai

2019

Adv Healthcare Materials

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“…14,15 The electrospinning nanofibers with very fine diameter and high porous structure have attracted increasing attention for their use in biomedical applications such as medical dressings, 16,17 tissue engineering scaffold and controlled drug delivery. 18 In this study, fish collagen was obtained from tilapia skin.…”

Section: Introductionmentioning

confidence: 99%

Multifunctional and biomimetic fish collagen/bioactive glass nanofibers: fabrication, antibacterial activity and inducing skin regeneration in vitro and in vivo

Zhou¹,

Sui²,

Mo³

et al. 2017

IJN

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The development of skin wound dressings with excellent properties has always been an important challenge in the field of biomedicine. In this study, biomimetic electrospun fish collagen/bioactive glass (Col/BG) nanofibers were prepared. Their structure, tensile strength, antibacterial activity and biological effects on human keratinocytes, human dermal fibroblasts and human vascular endothelial cells were investigated. Furthermore, the Sprague Dawley rat skin defect model was used to validate their effect on wound healing. The results showed that compared with pure fish collagen nanofibers, the tensile strength of the Col/BG nanofibers increased to 21.87±0.21 Mpa, with a certain degree of antibacterial activity against Staphylococcus aureus . It was also found that the Col/BG nanofibers promoted the adhesion, proliferation and migration of human keratinocytes. Col/BG nanofibers induced the secretion of type one collagen and vascular endothelial growth factor by human dermal fibroblasts, which further stimulated the proliferation of human vascular endothelial cells. Animal experimentation indicated that the Col/BG nanofibers could accelerate rat skin wound healing. This study developed a type of multifunctional and biomimetic fish Col/BG nanofibers, which had the ability to induce skin regeneration with adequate tensile strength and antibacterial activity. The Col/BG nanofibers are also easily available and inexpensive, providing the possibility for using as a functional skin wound dressing.

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“…A number of natural and synthetic materials have been studied as hydrogel‐based wound dressings. With regards to this, natural biopolymers, collagen, alginate, chitosan, cellulose acetate and hyaluronic acid were explored in terms of biocompatible properties and their potential role as a wound dressing material. In this context, alginate‐based dressing material has a pharmacological function, which is because of calcium ions used as a cross‐linking agent.…”

Section: Introductionmentioning

confidence: 99%

Biodegradable hydrogel‐based biomaterials with high absorbent properties for non‐adherent wound dressing

Kumar

Wang

Nune

et al. 2017

International Wound Journal

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Dressing materials involve conventional gauzes and modern materials such as hydrogels and foam-based biomaterials. Although the choice of dressing material depends on the type of wound, a dressing material is expected to be non-cytotoxic. Additionally, moist dressing is considered appropriate to accelerate epithelialisation, while dry dressing may cause tissue damage during removal. An ideal dressing material is expected to provide a moist environment and degrade and release the drug for faster wound healing. Thus, we have designed a hydrogel-based biodegradable dressing material to provide the moist environment with no cytotoxic effect in vitro. The design of the hydrogel involved alginate-collagen reinforced with whisker cellulose derived from cotton. The hydrogel was prepared via amide linkage in the presence of 1-ethyl-(dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysulfosuccinimide (NHS), followed by divalent cationic cross-linking of alginate and hydrogen bonding with cellulose. The high water retention capability of the hydrogel enables a moist environment to be maintained in the wounded area. The constituents of the hydrogel provided a microenvironment that was suitable for cell proliferation in the vicinity of the hydrogel but inhibited cell attachment on it. The MTT assay results indicated a higher fibroblast proliferation and viability in the presence of the hydrogel.

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Drug release, cell adhesion and wound healing evaluations of electrospun carboxymethyl chitosan/polyethylene oxide nanofibres containing phenytoin sodium and vitamin C

Cited by 54 publications

References 38 publications

Dendritic Effects of Injectable Biodegradable Thermogels on Pharmacotherapy of Inflammatory Glaucoma‐Associated Degradation of Extracellular Matrix

Dendritic Effects of Injectable Biodegradable Thermogels on Pharmacotherapy of Inflammatory Glaucoma‐Associated Degradation of Extracellular Matrix

Multifunctional and biomimetic fish collagen/bioactive glass nanofibers: fabrication, antibacterial activity and inducing skin regeneration in vitro and in vivo

Biodegradable hydrogel‐based biomaterials with high absorbent properties for non‐adherent wound dressing

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