Drug targeting in <i>Leishmania donovani</i> infections using tuftsin‐bearing liposomes as drug vehicles

Guru, P. Y.; Agrawal, Ajay; Singha, U. K.; Singhal, Arun; Gupta, C. M.

doi:10.1016/0014-5793(89)80222-4

Cited by 65 publications

(29 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Tuftsin potentiates the natural killer activity of the macrophages and other phagocytosing cells and has been shown to enhance the nonspecific resistance of the host against parasitic and fungal infections (4,6,12). However, in the present study the immunoprophylactic treatment with Tuft-Lip did not prove very effective against tuberculosis infections.…”

Section: Discussioncontrasting

confidence: 72%

See 1 more Smart Citation

Tuftsin-bearing liposomes as rifampin vehicles in treatment of tuberculosis in mice

Agarwal

Kandpal

Gupta

et al. 1994

Antimicrob Agents Chemother

View full text Add to dashboard Cite

The antitubercular activity of rifampin was considerably increased when it was encapsulated in egg phosphatidylcholine liposomes. A further increase in the activity was observed when the macrophage activator tetrapeptide tuftsin was grafted on the surface of the drug-loaded liposomes. Intermittent treatments (twice weekly) with these preparations were significantly more effective than the continuous treatments. Rifampin delivered twice weekly for 2 weeks in tuftsin-bearing liposomes was at least 2,000 times more effective than the free drug in lowering the load of lung bacilli in infected animals. However, pretreatment with drug-free tuftsin-bearing liposomes did not render the pretreated animals resistant to the Mycobacterium tuberculosis infections, neither did it appreciably increase the chemotherapeutic efficacy of the liposomized rifampin. These results clearly demonstrate that liposome targeting to macrophages could considerably increase the antitubercular activity of liposomized drugs such as rifampin. Also, it shows that immunoprophylactic treatment with macrophage activators such as tuftsin does not afford any advantage in treatment of tuberculosis infections,

show abstract

Section: Discussioncontrasting

confidence: 72%

“…The liposomes thus formed have been shown to be quite effective as drug vehicles in treatment of leishmaniasis (6) and aspergillosis (12). Also, pretreatment of animals with these liposomes has been found to render them resistant to a variety of infections (4,6,12 …”

mentioning

confidence: 99%

Tuftsin-bearing liposomes as rifampin vehicles in treatment of tuberculosis in mice

Agarwal

Kandpal

Gupta

et al. 1994

Antimicrob Agents Chemother

View full text Add to dashboard Cite

show abstract

“…with 250 μg·kg −1 per day of DOX and all its formulations for 4 consecutive days. After 30 days of treatment, splenic biopsies were performed and parasite burden was determined by counting the number of amastigotes (Guru et al, 1989).…”

Section: Measurement Of No Production In Infected Macrophagesmentioning

confidence: 99%

Coating doxorubicin‐loaded nanocapsules with alginate enhances therapeutic efficacy against Leishmania in hamsters by inducing Th1‐type immune responses

Kansal

Tandon

Verma

et al. 2014

British J Pharmacology

View full text Add to dashboard Cite

BACKGROUND AND PURPOSEThe aim of the present study was to evaluate the immunomodulatory and chemotherapeutic potential of alginate-(SA) coated nanocapsule (NCs) loaded with doxorubicin (SA-NCs-DOX) against visceral leishmaniasis in comparison with nano-emulsions containing doxorubicin (NE-DOX). EXPERIMENTAL APPROACHNE-DOX was prepared using low-energy emulsification methods. Stepwise addition of protamine sulphate and SA in a layer-by-layer manner was used to form SA-NCs-DOX. SA-NCs-DOX, NE-DOX and Free DOX were compared for their cytotoxicity against Leishmania donovani-infected macrophages in vitro and generation of T-cell responses in infected hamsters in vivo. KEY RESULTSSize and ζ potential of the NE-DOX and SA-NCs-DOX formulations were 310 ± 2.1 nm and (−)32.6 ± 2.1 mV, 342 ± 4.1 nm and (−)29.3 ± 1.2 mV respectively. SA-NCs-DOX was better (1.5 times) taken up by J774A.1 macrophages compared with NE-DOX. SA-NCs -DOX showed greater efficacy than NE-DOX against intramacrophagic amastigotes. SA-NCs-DOX treatment exhibited enhanced apoptotic efficiency than NE-DOX and free DOX as evident by cell cycle analysis, decrease in mitochondrial membrane potential, ROS and NO production. T-cell responses, when assessed through lymphoproliferative responses, NO production along with enhanced levels of iNOS, TNF-α, IFN-γ and IL-12 were found to be up-regulated after SA-NCs-DOX, compared with responses to NE-DOX in vivo. Parasitic burden was decreased in Leishmania-infected hamsters treated with SA-NCs-DOX, compared with NE-DOX. CONCLUSIONS AND IMPLICATIONSOur results provide insights into the development of an alternative approach to improved management of leishmaniasis through a combination of chemotherapy with stimulation of the innate immune system.

show abstract

“…To remove free drug, liposomes with entrapped SAG were washed in PBS with centrifugation (10,000 ϫ g, 30 min, 4°C) (15). Approximately 300 to 400 g of SAG was associated with 22 mg of lipid, estimated as described previously (2,17).…”

mentioning

confidence: 99%

Combination Therapy Using Sodium Antimony Gluconate in Stearylamine-Bearing Liposomes against Established and ChronicLeishmania donovaniInfection in BALB/c Mice

Pal

Ravindran

Ali

2004

Antimicrob Agents Chemother

View full text Add to dashboard Cite

show abstract

Drug targeting in Leishmania donovani infections using tuftsin‐bearing liposomes as drug vehicles

Cited by 65 publications

References 5 publications

Tuftsin-bearing liposomes as rifampin vehicles in treatment of tuberculosis in mice

Tuftsin-bearing liposomes as rifampin vehicles in treatment of tuberculosis in mice

Coating doxorubicin‐loaded nanocapsules with alginate enhances therapeutic efficacy against Leishmania in hamsters by inducing Th1‐type immune responses

Combination Therapy Using Sodium Antimony Gluconate in Stearylamine-Bearing Liposomes against Established and ChronicLeishmania donovaniInfection in BALB/c Mice

Contact Info

Product

Resources

About